P62 ab109012

Manufactured by Abcam

Sourced in United States

The P62 (ab109012) is a recombinant protein product that can be used as a standard control in western blotting and other immunoassays. It is a well-characterized protein that serves as a marker for autophagy and cellular stress response pathways.

Automatically generated - may contain errors

Lab products found in correlation

D glucose, by Merck Group (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions) N acetylcysteine, by MedChemExpress (1 mentions) Bcl 2 ab196495, by Abcam (1 mentions) Aicar, by MedChemExpress (1 mentions) Ab6276, by Abcam (1 mentions) Tris glycine sds buffer, by Thermo Fisher Scientific (1 mentions) Phospho ulk1 s757, by Cell Signaling Technology (1 mentions) Phospho p70s6k t389, by Cell Signaling Technology (1 mentions) Tris glycine gel, by Bio-Rad (1 mentions) Reactive oxygen species assay kit, by Beyotime (1 mentions) Tunel assay kit, by Roche (1 mentions) Lc3b ab51520, by Abcam (1 mentions) Beclin1 11306 1 ap, by Proteintech (1 mentions) Pink 1 23274 1 ap, by Proteintech (1 mentions)

Spelling variants of this product

Ab109012 (154 citations) Anti-p62 (ab109012) (3 citations)

3 protocols using p62 ab109012

Molecular Mechanisms of Cell Stress Response

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Primary antibodies including p62 (ab109012), LC3 (ab62721), B-cell lymphoma 2 (Bcl-2, ab196495), p16 (ab51243), p21(ab109199) and β-actin (ab6276) were acquired from Abcam (Cambridge, MA, USA). Primary antibodies against p-AMPK (50081), AMPK (5831), Bax (2772), and p53 (2524) were purchased from Cell Signaling Technologies (CST; Danvers, MA, USA). Primary antibodies against cleaved caspase-3 (C-caspase-3, WL01992) were purchased from WanleiBio (Shen Yang, China). Goat anti-rabbit IgG (7074) and goat anti-mouse IgG (7076) secondary antibodies were purchased from CST (Danvers, MA, USA). Cell culture reagents were purchased from Gibco (Grand Island, NY, USA). D-(+)-Glucose was purchased from Sigma-Aldrich (St. Louis, MO, USA). AICAR and Acetylcysteine also called N-acetyl cysteine (NAC) were acquired from MedChemExpress (MCE; Manmouth Junction, NJ, USA).

Wang B., Shi Y., Chen J., Shao Z., Ni L., Lin Y., Wu Y., Tian N., Zhou Y., Sun L., Wu A., Hong Z., Wang X, & Zhang X. (2021). High glucose suppresses autophagy through the AMPK pathway while it induces autophagy via oxidative stress in chondrocytes. Cell Death & Disease, 12(6), 506.

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Western Blot Analysis of mTOR Signaling

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Myocardial lysates were derived from flash frozen tissue (Cell Signaling Technology #9803) and protein concentration determined by BCA (Pierce). Samples were prepared in SDS Tris-Glycine buffer (Life Technologies) and run on TGX 7.5% or 4–15% Tris-Glycine Gels (Bio-Rad) and blotted onto a nitrocellulose membrane. The following primary antibodies were used in this study: phospho-p70 S6K (T389) #9205 lot 26 used at 1:1,000, p70 S6K #9202 lot 20 used at 1:1,000, phospho-4EBP1 (S65) #9451 lot 14 used at 1:1,000, 4EBP1 #9452 lot 12 used at 1:1,000, phospho-Ulk-1 (S757) #1420 clone D706U lot 4 used at 1:500, Ulk-1 #8054 clone D8H5 lot 5 used at 1:500, TSC2 #3612 clone D93F12 lot 6 used at 1:1,000 (Cell Signaling Technology), phospho-TSC2 (S1365) #120718 lot NFSA12072OAH used at 1:500 (NovoPro Labs), and P62 #ab109012 lot GR12843–70 used at 1:1,000 (Abcam), and a total protein stain #926–11016 lot C80522–02 used at 5 ml/membrane (Li-Cor). Antibody binding was visualized by infrared imaging (Odyssey, Li-Cor) and quantified with Li-Cor Image Studio Software 3.1.

Oeing C.U., Jun S., Mishra S., Dunkerly-Eyring B.L., Chen A., Grajeda M.I., Tahir U.A., Gerszten R.E., Paolocci N., Ranek M.J, & Kass D.A. (2021). MTORC1-Regulated Metabolism Controlled by TSC2 Limits Cardiac Reperfusion Injury. Circulation research, 128(5), 639-651.

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Assessing Cellular Stress and Apoptosis

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2,3,5-Triphenyl Tetrazolium Chloride (TTC) was obtained from Sigma. TUNEL Assay Kit was purchased from Roche (Mannheim, Germany). A Reactive Oxygen Species Assay Kit was obtained from Beyotime (Jiangsu, China). Antibodies against Caspase 3, cleaved Caspase 3, apoptosis protease-activating factor-1 (Apaf-1), and cytochrome c (Cyto c) were purchased from Cell Signaling Technology (Danvers, MA). LC3B (ab51520) and p62 (ab109012) antibodies were purchased from Abcam. Beclin1 (11306-1-AP), PINK1 (23274-1-AP), and MnSOD (24127-1-AP) antibodies were purchased from Proteintech. Anti-β-actin antibody was purchased from Bios (Beijing, China).

He M., Zhang T., Fan Y., Ma Y., Zhang J., Jing L, & Li P.A. (2020). Deletion of mitochondrial uncoupling protein 2 exacerbates mitophagy and cell apoptosis after cerebral ischemia and reperfusion injury in mice. International Journal of Medical Sciences, 17(17), 2869-2878.

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