Sirna sc 37007

Manufactured by Santa Cruz Biotechnology

Sourced in France

SiRNA (sc-37007) is a small interfering RNA (siRNA) product developed by Santa Cruz Biotechnology. siRNA is a class of double-stranded RNA molecules that can inhibit the expression of specific genes by neutralizing target mRNA. The core function of this particular siRNA is to provide a tool for gene silencing studies, allowing researchers to investigate the effects of suppressing the expression of target genes.

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Lab products found in correlation

Sirnas, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Sirna transfection reagent, by Santa Cruz Biotechnology (1 mentions) Antihuman erα hc 20 antibody sc 543, by Santa Cruz Biotechnology (1 mentions) P44 42 mapk sirna, by Cell Signaling Technology (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Fulvestrant, by Merck Group (1 mentions) Bx795 tlrl bx7, by InvivoGen (1 mentions) Anti β actin sc 81178, by Santa Cruz Biotechnology (1 mentions) Anti irf3 sc 9082, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Sc-37007 (308 citations) Control siRNA (sc-37007) (114 citations) SiRNAs (95 citations) Control siRNA-A (sc-37007) (21 citations) Scrambled siRNA (sc-37007) (17 citations) NC-siRNA (sc-37007) (12 citations) Control siRNAs (sc-37007) (7 citations) Scrambled control siRNA (sc-37007) (6 citations) Scramble siRNA (sc-37007 (5 citations) Scrambled siRNA-A (sc-37007) (2 citations)

4 protocols using sirna sc 37007

GFP-Tagging and CRISPR Editing of T6BP

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The GFP-T6BP plasmid was generated by subcloning T6BP ORF (accession #: BC050358.2) into pEGFP-C1 vector at restriction sites (BspE1 and KpnI). Mutant T6BP constructs (G715E, G668E, G636E, G522E, G687E, and G621E) were generated using custom gBLOCKS (IDT), and mutant fragments were cloned through restriction digestion with either EcoRI/KpnI or BglII/EcoRI. Cloning of truncated T6BP-ΔUBZ (Δ687-789) was performed using Kpn2I/KpnI restriction sites with the following primers (forward primer: AAA TTT TCC GGA ATG ACA TCC TTT CAA GAA GTC CCA TT; reverse primer: AAA TTT GGT ACC CTA ATC AGG CCG ACT AAA GTT TC). The single guide RNA (sgRNA) sequence targeting human T6BP is CATGTCATCTTTCAAAATG (Exon 2) and was cloned into pSpCas9-2A-GFP vector. The scrambled small interfering RNA (siRNA; sc-37007) and siRNAs targeting NDP52 (sc-93738), OPTN (sc-39054), and T6BP (sc-106831) were purchased from Santa Cruz Biotechnology. For transfection, cells were transiently transfected with plasmid cDNAs or sgRNAs using Lipofectamine 2000 (Invitrogen, 11668-019) following the manufacturer’s instructions.

Mohamud Y., Xue Y.C., Liu H., Ng C.S., Bahreyni A, & Luo H. (2021). Autophagy Receptor Protein Tax1-Binding Protein 1/TRAF6-Binding Protein Is a Cellular Substrate of Enteroviral Proteinase. Frontiers in Microbiology, 12, 647410.

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FOXP1 Gene Knockdown in Karpas miRZip-92a Cells

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5×10⁶ Karpas miRZip-92a cells, cultured in a 6-well Petri dish, were transfected with FOXP1 siRNA (sc-4483, Santa Cruz Biotechnology, France) or scrumble (siRNA sc-37007, Santa Cruz Biotechnology, France) in siRNA transfection reagent (sc-39528, Santa Cruz Biotechnology, France) according to the manufacturer’s protocol.

Romero M., Gapihan G., Castro-Vega L.J., Acevedo A., Wang L., Li Z.W., El Bouchtaoui M., Di Benedetto M., Ratajczak P., Feugeas J.P., Thieblemont C., Saavedra C, & Janin A. (2016). Primary mediastinal large B-cell lymphoma: transcriptional regulation by miR-92a through FOXP1 targeting. Oncotarget, 8(10), 16243-16258.

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Signaling Pathway Profiling Assay

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Antihuman MYC (#5605), p-MAPK (#9101), MAPK (#9102), p-AKT (Ser473) (#9271), AKT (#9272), p-ER (Ser167) (#5587), GAPDH (#2118) antibodies were obtained from Cell Signaling Technology. Antihuman HER2 (#06-562) and p-ERα (Ser118) (ab32396) antibodies were from Abcam Inc. Antihuman ERα (HC-20) antibody (sc-543) was from Santa Cruz Biotechnology. The ER antagonist fulvestrant (#14409) and the SLC1A5 inhibitor L-Glutamic acid γ-(p-nitroanilide) (GPNA) (G6133) were obtained from Sigma-Aldrich. The glutaminase inhibitor compound 968 (#352010) was from EMD Millipore. The AKT inhibitor MK-2206 (S1078) was from Selleck Chemicals. The nontargeting control siRNA (sc-37007) and MYC siRNA (sc-29226) were obtained from Santa Cruz Biotechnology. The HER2 siRNA (L-003126-00) was from Dharmacon. The p44/42 MAPK siRNA (#6560) was from Cell Signaling Technology.

Chen Z., Wang Y., Warden C, & Chen S. (2015). Cross-talk between ER and HER2 regulates c-MYC-mediated glutamine metabolism in aromatase inhibitor resistant breast cancer cells. The Journal of steroid biochemistry and molecular biology, 149, 118-127.

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Investigating Innate Immune Signaling Pathways

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Anti-IFI16 (SAB1408587), anti-DAI (PRS4401), and anti-cGAS (SAB3500110) antibodies were purchased from Sigma (St. Louis, MS, USA). Anti-phospho-IRF3 (number 4947) and anti-ISG15 (number 2743) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin (sc-81178), anti-IRF3 (sc-9082), and anti-Mx1 (sc-50509) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-TLR3 (ab137722), anti-TLR7 (ab45371), anti-TLR8 (ab24185), anti-TLR9 (ab12121), anti-MDA5 (ab69983), anti-RIG-I (ab45428), anti-OAS1 (ab86343), and anti-Pol III (ab22236) antibodies were purchased from Abcam (Cambridge, UK). Poly(I:C)/Lyovec™ (tlrl-piclv), HSV60/Lyovec (tlrl-hsv60c), and BX795 (tlrl-bx7) were purchased from InvivoGen (San Diego, CA, USA). Small interfering RNA (siRNA) targeting RIG-I (sc-61480), TLR3 (sc036685), and IFI16 (sc-166504) and control siRNA (sc-37007) targeting a scrambled sequence were purchased from Santa Cruz Biotechnology.

Yu L., Xu Y., Wang F., Yang C., Liu G, & Song X. (2016). Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells. BioMed Research International, 2016, 9872138.

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