P gcn2 ab75836

Manufactured by Abcam

Sourced in United Kingdom

P-GCN2 (ab75836) is a primary antibody that detects GCN2, a serine/threonine protein kinase involved in the cellular response to amino acid deprivation. The antibody can be used for western blotting applications.

Automatically generated - may contain errors

Lab products found in correlation

Image multi gauge software, by Fujifilm (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) Pakt t308, by Cell Signaling Technology (1 mentions) Mbs2538144, by MyBioSource (1 mentions) P nrf2 ab76026, by Abcam (1 mentions)

Spelling variants of this product

Ab75836 (6 citations) P-GCN2 (2 citations)

2 protocols using p gcn2 ab75836

Western Blot Analysis of Protein Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein samples were prepared using a radioimmunoprecipitation assay lysis buffer.²³ Samples were further heated and analyzed with SDS‐PAGE. After transferring and blocking, targeted proteins were detected with specific primary/secondary Abs and ECL reagents using GE Healthcare luminescence fluorescence image capture system (AI680). The MultiGauge image software (Fujifilm) was used for quantification. The PERK (sc‐32577) Ab was purchased from Santa Cruz Biotechnology. The p‐GCN2 (ab75836) and p‐PKR (phospho T446, ab32036) Abs were purchased from Abcam. Eukaryotic initiation factor 2α (AHO0802), p‐eIF2α (44‐728G), GFRAL (PA5‐24545), and α‐tubulin (A11126) Abs were obtained from Invitrogen. Antibodies against GCN2 (#65981), PKR (#12297), PERK (#5683), xCT (#12691), Akt (#4685), p‐Akt T308 (#2965), p‐Akt S473 (#9271), STAT3 (#9139), p‐STAT3 (#9145), ERK1/2 (#9101), and p‐ERK1/2 (#9102) were purchased from Cell Signaling Technology. Heme‐regulated eIF2α kinase Ab was purchased from MyBiosource (MBS2538144). Activating transcription factor 4 (10835‐1‐AP) Ab was obtained from Proteintech.

Wang S., Chang Y., Fang W., Li A.F., Chen C., Yeh T., Hung G., Huang K, & Lee H. (2023). Growth differentiation factor 15 induces cisplatin resistance through upregulation of xCT expression and glutathione synthesis in gastric cancer. Cancer Science, 114(8), 3301-3317.

+ Open protocol

+ Expand

Investigating Cellular Stress Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

ATF4 (# 11815), GCN2 (# 3302), eIF2α (# 9722), NRF2 (# 12721), PERK (#3192) and normal rabbit IgG (# 7074) antibodies were purchased from Cell Signaling Technology (Beverly, MA). P-GCN2 (ab75836), P-NRF2(ab76026) and P-eIF2α (ab32157) antibodies was obtained from Abcam (Cambridge, UK). GAPDH (G9545) antibody was from Sigma-Aldrich (Saint Louis, MO, USA).

Carraro V., Combaret L., Coudy-Gandilhon C., Parry L., Averous J., Maurin A.C., Jousse C., Voyard G., Fafournoux P., Papet I, & Bruhat A. (2022). Activation of the eIF2α-ATF4 Pathway by Chronic Paracetamol Treatment Is Prevented by Dietary Supplementation with Cysteine. International Journal of Molecular Sciences, 23(13), 7196.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!