A02082003by

Experiments were performed in accordance with the Institutional Animal Care and Use Committee guidelines with its approvals. The LKO mice were generated by crossing Albumin-CreTg/+ mice (Jackson Laboratories) with mice homozygous for a “floxed” exon 6 of IPMK (IPMK fl/fl). The control mice for this study were IPMK fl/fl (WT) mice. Mice were housed under standard conditions in a temperature- and humidity-controlled facility with a light–dark cycle of 12 h (lights on at 07:00) and fed ad libitum at the Johns Hopkins University (Baltimore, MD, USA).
Experiment 1: Eight-week-old WT mice were fed an ND (A02082003BY, Research Diets, New Brunswick, NJ, USA) or MCDD (A02082002BR, Research Diets, New Brunswick, NJ, USA) for 2 weeks.
Experiment 2: Eight-week-old WT and LKO mice were fed MCDD for 2 weeks.
Experiment 3: Ten-week-old WT mice were divided into two groups. One group (ALF) had ad libitum access to the MCDD diet, while the other group underwent time-restricted feeding (TRF), with access to the MCDD diet from 7 p.m. to 8 a.m., for two weeks.
All mice were sacrificed after a 5 h fast (from 08:00 a.m. to 1:00 p.m.), and blood was collected from the heart before harvesting liver tissues.

Standard chow was replaced in 12-week-old male mice with methionine and choline-deficient (MCD) diet (A02082002BR Research Diets, U.S.A.) or the matched control diet (A02082003BY, Research Diets, U.S.A.). Dietary modification persisted for either 2 or 4 weeks, at which point mice were humanely sacrificed by cervical dislocation and liver tissue was harvested and used for downstream analysis.