The morphology of the NH2KIT-6 filler was analyzed by FESEM (VPFESEM, Zeiss Supra55 VP). NH2KIT-6 were subjected to XRD (X’Pert3 Powder & Empyrean, PANalytical) scanning for crystalline structure study. Functional groups in KIT-6 and NH2KIT-6 were determined by FTIR (Perkin Almer, Frontier). The pore characteristics of KIT-6 and NH2KIT-6 were analyzed using N2 adsorption–desorption analysis (TriStar II 3020 V1.04) with liquid nitrogen at 77 K. The specific surface area of the sample was calculated by using the Brunauer–Emmett–Teller (BET) method. The mesopore size distribution was determined by using the Barrett–Joyner–Halenda (BJH) method. The morphology of the fabricated membranes was analyzed by FESEM (VPFESEM, Zeiss Supra55 VP). The membranes were also subjected to FTIR analysis (Perkin Almer, Frontier).
Vpfesem
Manufactured by Zeiss
Sourced in Germany, Switzerland
The VPFESEM is a high-resolution scanning electron microscope (SEM) designed for advanced materials analysis. It features a field emission electron source and vacuum-compatible sample chamber, enabling high-resolution imaging and elemental analysis of a wide range of materials.
Automatically generated - may contain errors
Lab products found in correlation
Supra 55vp, by Zeiss (6 mentions)
Oca20, by Dataphysics (2 mentions)
Nanonavi e sweep, by Anton Paar (1 mentions)
Labram hr uv vis nir, by Horiba (1 mentions)
X pert3 powder empyrean, by Malvern Panalytical (1 mentions)
D2 phaser diffractometer, by Bruker (1 mentions)
Uv 1800, by Shimadzu (1 mentions)
10 protocols using vpfesem
1
Physicochemical Characterization of Amino-Functionalized KIT-6 Silica
2
Visualizing Multispecies Biofilms by SEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The glass slips with single-species and multispecies biofilm after 24 hours incubation were observed by scanning electron microscopy (VP-FE-SEM, CarlZeiss, Oberkochen, Germany). The glass slips with single-species and multispecies biofilms were washed twice with PBS. Glass slips with attached bacteria were fixed in 2.5% glutaraldehyde in PBS (pH 7.4) for 1 hour at room temperature. The fixed samples were washed three times with PBS for 10 minutes and dehydrated for 30 minutes in a graded series of ethanol. After critical point drying, the samples were mounted on stubs, coated with gold, and observed with SEM. Glass slips with single- and multispecies biofilms were observed by SEM (×10,000, ×30,000).
3
Comprehensive Membrane Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The membrane was characterized in terms of surface morphology, porosity, hydrophilicity (contact angle), surface roughness and mechanical strength. A field emission scanning electron microscope (FESEM, Model: VPFESEM, Zeiss Supra55 VP, Feldbach, Switzerland) was used to observe the morphology of the membrane. All samples were mounted onto a metal substrate using carbon tape and coated with a thin layer of gold. For pore size measurement, ImageJ software was used. Furthermore, for porosity measurement, the dry wet method was used where the membrane weight and volume were measured. Membrane hydrophilicity (contact angle) was measured by using a goniometer via the Sessile Drop Method (IFT, Model: OCA 20, Data Physics, Filderstadt, Germany). To analyse surface roughness, an atomic force microscope (AFM, Model: NanoNavi E-Sweep Anton Paar, GmbH, Graz Austria) was used. The mechanical strength of the membrane was tested according to the ASTM standard D638 with a crosshead speed of 10 mm/min by using Universal Testing Machine (UTM, Shimadzu, Nakagyo-ku, Kyoto, Japan). The membranes were cut with a dimension of 30 mm × 70 mm and were mounted with an aluminum plate at both ends for a better grip.
4
Characterization of Nanostructured Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Powder X-ray diffraction (XRD) measurement was performed on a diffractometer (XRD, GBC eMMA), which was operated in the reflection mode with Cu Kα radiation (28 mA, 35 kV) at a step size of 1° min−1 in the range of 10–80°. The surface morphology and elemental composition of each sample was characterized using Scanning Electron Microscopy (SEM) (Zeiss 1555, VP-FESEM) at an accelerating voltage of 5 kV with energy dispersive X-ray spectrometry (EDS). The samples were loaded onto the carbon tape and coated by platinum. Transmission Electron Microscopy (TEM) analysis was performed on TEM-TITAN operating at 200 kV. The samples were dispersed in ethanol under ultrasonication and loaded onto carbon copper grid for TEM characterization. Nitrogen adsorption–desorption isotherms were measured at 77 K using SAPA2010 (Micromeritics Inc., USA). The samples were degassed at 200 °C overnight under vacuum.
5
Comparative SEM Analysis of Transgenic Jute Fibers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Surface morphological features of the transgenic and control jute fibers were observed by SEM (Zeiss sigma VP FE-SEM) and compared to analyze lignin reduction in all transgenic lines. Five elementary jute fiber samples from each transgenic and control jute plants were carefully cut into small pieces of similar size and mounted with adhesive tape on stubs, sputter-coated with gold and then examined under SEM operated at 10kV. Repeated images were taken for each transgenic and control sample to confirm the reproducibility of the result.
6
Comprehensive Membrane Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Membrane characterization was based on surface morphology, functional group, hydrophilicity and porosity. Field Emission Scanning Electron Microscope (FESEM, Model: VPFESEM, Zeiss Supra55 VP, Feldbach, Switzerland) was used to observe the membrane surface morphology. The sample was coated with gold after being mounted onto a metal substrate. Fibre diameter and pore size were measured by using ImageJ Software (ImageJ 1.53e, Bethesda, MD, USA). Additionally, the functional group presence in the membrane was analysed by using Fourier Transform Infrared Spectroscopy (FTIR, Model: Thermo-Nicolet, Waltham, MA, USA) and compared with result from our previous reports [49 ]. Goniometer was used to measure contact angle via Sessile Drop Method (IFT, Model: OCA 20, Data Physics, Filderstadt, Germany). The contact angles were measured three times by using the build-in software Interfacial Tension (SCA 20, Filderstadt, Germany). Furthermore, porosity was measured by using dry-wet method in which the membrane weight and volume were measured and calculated [50 (link)].
7
Scanning Electron Microscopy of FAMS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Scanning electron microscopy (SEM) was accomplished by drying samples (20 μL) of FAMS, protein-coated FAMS, and E. coli – loaded FAMS on 12.7 mm Aluminum specimen mounts (Ted Pella, Inc., 6 mm Pin, Zeiss). Samples were gold-palladium coated using a Bal-tec SCD-050 sputter coater. Micrographs were obtained using a Zeiss VP-FESEM with electron high tension of 3.5 kV and working distance 5.3 mm. Facilities used for SEM were courtesy of the Huck Institutes of Life Sciences Microscopy Facility at The Pennsylvania State University. Images were false colored in Adobe Photoshop where indicated.
8
Characterization of Fabricated Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The surface morphology of the fabricated scaffolds was characterized
by using a field emission scanning electron microscope (SIGMA VP FESEM,
ZEISS). The crystallinity of the scaffolds was tested by an X-ray
diffractometer (Rigaku, 007HF, Japan) with Cu Kα radiation (λ
= 1.54 Å) at room temperature at an angular range of 10–60°
in 2θ, in steps of 0.050°. Chemical compositional analysis
was conducted using a Fourier transform infrared (FT-IR) spectrophotometer
(Bruker VERTEX 70 FT-IR spectrophotometer, Germany) and a micro-Raman
spectrometer (LabRAM HR UV–vis NIR, Horiba) to record the FT-IR
and Raman spectra, respectively. Mechanical properties of the scaffolds
were assessed using a Universal Testing Machine (Tinius Olsen 5ST)
equipped with a 2.5 kN load cell at a crosshead rate of 1 mm/min and
a gauge length of 20 mm, following a standard ASTM D638 procedure.
Steady-state current–voltage (I–V) measurements were performed using a Keithley 2450 source
meter using a two-probe technique at a DC voltage sweep from −10
and +10 V at room temperature.
by using a field emission scanning electron microscope (SIGMA VP FESEM,
ZEISS). The crystallinity of the scaffolds was tested by an X-ray
diffractometer (Rigaku, 007HF, Japan) with Cu Kα radiation (λ
= 1.54 Å) at room temperature at an angular range of 10–60°
in 2θ, in steps of 0.050°. Chemical compositional analysis
was conducted using a Fourier transform infrared (FT-IR) spectrophotometer
(Bruker VERTEX 70 FT-IR spectrophotometer, Germany) and a micro-Raman
spectrometer (LabRAM HR UV–vis NIR, Horiba) to record the FT-IR
and Raman spectra, respectively. Mechanical properties of the scaffolds
were assessed using a Universal Testing Machine (Tinius Olsen 5ST)
equipped with a 2.5 kN load cell at a crosshead rate of 1 mm/min and
a gauge length of 20 mm, following a standard ASTM D638 procedure.
Steady-state current–voltage (I–V) measurements were performed using a Keithley 2450 source
meter using a two-probe technique at a DC voltage sweep from −10
and +10 V at room temperature.
9
Nanomaterial Characterization for Biosensing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In addition to electrochemical measurements, other features of the nanomaterial and the biosensor were tested using supplementary techniques. Decoration and dispersion of nanomaterials on the GCE surface were evaluated with a Scanning Electron Microscope (SEM) using SIGMA instrument model VP FE-SEM (Zeiss SIGMA, Oberkochen, Germany). Energy Dispersive Spectroscopy (EDS) was used for elemental analysis of the surface using the SEM instrument. Fourier transform infrared (FTIR) spectroscopy was performed to assess the chemical bonds within and between applied nanomaterials using an Avatar 360 instrument (Thermal Nicolet, Nicolet, QC, Canada).
10
Characterization of BiOBr-Based Nanomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The X-ray diffraction (XRD) patterns using a Bruker D2-Phaser Diffractometer (Coventry, UK) with a Cu Kα radiation source (λ = 1.5418 Å) were recorded. The surface morphology and elemental analysis of BiOBr-G and BiOBr-C were examined by Zeiss-Sigma VP FESEM, Ostalbkreis, Germay (field emission scanning electron microscope), FEI Technai G2 X-Twin TEM, Malaga, Spain (transmission electron microscope), 200 kV and EDS (energy dispersive X-ray spectroscopy), Bruker, Coventry, UK. Surface area was estimated by the BET method for which nitrogen adsorption–desorption studies were carried out at 77 K using a Quanta Chrome NOVA 1000, Graz, Austria. XPS (X-ray photoelectron spectroscopy) data were obtained on a PHOIBOS (150 MCD) device (Berlin, Germany) with 1486.69 eV, Al Kα monochromatic radiation at 20 mA and 14 kV, and the pressure <10−9 mbar. The functional groups on the as-prepared samples were determined using FTIR (Agilent, Cary 630, NC, USA). The optical characteristics were analyzed by both UV-vis diffuse reflectance spectra (Shimadzu UV-1800, Columbia, SC, USA) and photoluminescence spectra (Shimadzu RF-5301, Columbia, SC, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!