A GTT and PTT was performed in mice that had been deprived of food for 16 h. Glucose (1 g/kg) or sodium pyruvate (1.5 g/kg) was administered intraperitoneally, and the blood glucose concentration was measured at various times thereafter using Free Style Lite Blood Glucose test strips (Abbott Laboratories). An insulin tolerance test was performed in mice that had been deprived of food for 5 h. Insulin (0.1 U/kg; Humulin R, Eli Lilly) was injected intraperitoneally, and the blood glucose concentration at various times thereafter was measured, as in the GTT.
Freestyle lite blood glucose test strips
Manufactured by Abbott
Sourced in United Kingdom, Germany
The FreeStyle Lite blood glucose test strips are designed for use with the FreeStyle Lite blood glucose monitoring system to measure the level of glucose in blood samples.
Automatically generated - may contain errors
4 protocols using freestyle lite blood glucose test strips
1
Glucose and Insulin Tolerance Tests
2
Streptozotocin-Induced Diabetes in Mice
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Diabetes was induced in 12-week-old male C57BL/6J mice (~25 g) by five daily intraperitoneal injections of 50 mg/kg streptozotocin (STZ) in freshly prepared 0.1 M citrate buffer (pH 4.5). Control animals received citrate buffer alone. One week after injections, glucose level was determined using FreeStyle Lite Blood Glucose Test strips (Abbott, Oxfordshire, UK). Blood glucose levels and weights were monitored biweekly throughout the study. Glycated haemoglobin (Hba1c) levels were assessed at the end of the experiment using the A1CNow+ system (PTS Diagnostics, Indianapolis, IN, USA), according to manufacturer’s instructions.
3
Serum Biomarker Measurement Protocol
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Serum Insulin-like growth factor 1 (IGF1) concentrations were measured using the iSYS automated chemiluminescent IGF1 assay (Immunodiagnostic Systems) as described previously [27] . Blood glucose levels were immediately determined in duplicate from freshly collected full blood using a FreeStyle Freedom Lite blood glucose meter (Abbott) and FreeStyle Lite blood glucose test strips (Abbott) [28] . After clotting for 30 min at room temperature and centrifugation (1200×g, 20 min, 4 °C), aliquots of serum were stored at -80 °C. Insulin levels were determined from serum with a sandwich chemiluminescence immunoassay (LIAISON® Insulin, DiaSorin) in combination with a fully automated immunoassay analyzer (LIAISON® XL, DiaSorin). Nonesterified free fatty acids (NEFAs) were analyzed from EDTA plasma using an AU480 clinical chemistry analyzer (Beckman Coulter) and adapted reagent kits from FUJIFILM Wako Chemicals GmbH as described previously [29] .
4
Islet Transplantation in Diabetic NSG Mice
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After induction of diabetes by intraperitoneal injection of 180 mg/kg body weight streptozotocin, diabetic NSG mice (blood glucose levels >350 mg/dl) received native NPICCs (3000, 1500, or 750 IEQs/mouse) or REPIs (750 or 1500 IEQs/mouse) under the left kidney capsule as described recently (22 (link), 26 (link)). 3000 IEQs represent the standard dose of NPICCs for transplantation to achieve normoglycemia in about 80% of diabetic NSG mice. Blood glucose levels were monitored by FreeStyle Lite blood glucose test strips (Abbott, Wiesbaden, Germany). Diabetic mice with blood glucose levels >300 mg/dl were treated with insulin glargine (0.25–1 IE s.c. daily). Our primary endpoint was normoglycemia. The observation period was set to a maximum of 16 weeks. We used a stringent definition for normoglycemia which was specified as achievement of pretransplant glycemia (persistent random non-fasting blood glucose levels <120 mg/dl). This cut-off is based on the measurement of non-fasting blood glucose levels of untreated NSG mice (n = 107; 94.6 ± 15.2 mg/dl, range 62–128 mg/dl). In this control cohort there was no animal with non-fasting blood glucose levels above 120 mg/dl on two consecutive days. Because other studies often used non-fasting blood glucose levels <180 mg/dl to define normoglycemia after islet transplantation this threshold was also analyzed.
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