Tbxa2r

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom, United States

TBXA2R is a laboratory measurement device for the quantitative detection and analysis of thromboxane A2 receptor (TBXA2R) protein expression levels in biological samples. The core function of TBXA2R is to provide accurate and reliable data regarding the presence and quantity of TBXA2R, which is a key regulator of various physiological processes.

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Lab products found in correlation

Image lab software, by Bio-Rad (1 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Cox 2, by Abcam (1 mentions) Immobilon p pvdf membrane, by Merck Group (1 mentions) Ptgfr, by Abcam (1 mentions) β actin, by Merck Group (1 mentions)

Spelling variants of this product

29-mer small hairpin RNA (shRNA) constructs against human TBXA2R and TBXAS1 (2 citations)

2 protocols using tbxa2r

Quantification and Visualization of Protein Levels

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The BCA assay quantified proteins, and loading buffer 5X was added to the proteins, which were incubated for 5 min at 95°C. Then, proteins were loaded on an SDS-PAGE polyacrylamide gel, transferred to Immobilon-P PVDF membrane (Millipore, Shanghai, China), probed with the appropriate primary antibodies: COX-1 (1:5000), COX-2 (1:4000), and PTGFR (1:2000) were obtained from Abcam (Cambridge, UK), and TBXA2R (1:4000), GAPDH (1:10000), and detected by chemiluminescence (ECL, Thermo Scientific). Images were then acquired with Image-Lab software (Bio-Rad, Hercules, CA, USA). Image analysis of western blots was performed with Image-Lab analyzer software.

Li M., Haixia Y., Kang M., An P., Wu X., Dang H, & Xu X. (2021). The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats. Frontiers in Endocrinology, 12, 652807.

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Protein Expression Analysis of Vascular Segments

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Cleaned from connective and fat tissue, supraceliac, paravisceral, and juxtarenal segments were stored at −80°C. For WB analysis, 5–6 segments per group were pulled, homogenized in RIPA buffer, and lysates were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The following primary antibodies were used for WB analysis: α_1A-AR (#sc-100291, SCBT, TA, USA), TBXA2R (#PA5-67751, Thermo Scientific, Waltham, MA, USA), and β-actin (#A2228, Sigma, St. Louis, MO, USA).

Aslanidou L., Trachet B., Sasset L., Lovric G., Stergiopulos N, & Di Lorenzo A. (2020). Early Morphofunctional Changes in Angll-Infused Mice Contribute to Regional Onset of Aortic Aneurysm and Dissection. Journal of Vascular Research, 57(6), 367-375.

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