Cells (1 × 104/well) were seeded on 8-well chamber slides (Lab-Tech) after transfection. The cells were fixed, permeated and blocked. Then, they were incubated with the following antibodies: anti-VIM (1:200, Abcam), anti-ITGAV (1:1000, Cell Signaling Technology), anti-ITGB1 (1:1000, Cell Signaling Technology), anti-ITGB3 (1:500, Cell Signaling Technology) or anti-CD47 (1:200, Abcam). The secondary antibody, Alexa Fluor 488-conjugated goat anti-mouse IgG or anti-rabbit IgG (1:500, Invitrogen), was applied for 1 hour. Slides were mounted with antifading solution containing 4′-6′-diamidino-2-phenyl-indole (Vector Laboratories). Images were taken using a confocal microscope (Zeiss). All experiments were conducted in triplicate.
Anti itgav
Manufactured by Cell Signaling Technology
Anti-ITGAV is a primary antibody that recognizes the integrin subunit alpha V (ITGAV) protein. Integrins are a family of cell surface receptors that mediate cell-cell and cell-extracellular matrix interactions. Anti-ITGAV can be used to detect and study the ITGAV protein in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti itgb1, by Cell Signaling Technology (2 mentions)
Anti itgb3, by Cell Signaling Technology (2 mentions)
Anti cd47, by Abcam (2 mentions)
8 well chamber slide, by EQT (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions)
Alexa fluor 488 conjugated goat anti mouse igg or anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti vim, by Abcam (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Cell Signaling Technology (1 mentions)
Anti vim, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by Abcam (1 mentions)
Anti zeb2, by Abcam (1 mentions)
2 protocols using anti itgav
1
Immunofluorescence Analysis of Cell Adhesion Molecules
2
Western Blotting Analysis of Protein Expression
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Total protein was extracted from tissues and transfected cells using RIPA buffer including protease inhibitor cocktail (Cell Signaling Technology), and western blotting was carried out as described previously [42 (link)]. The primary antibodies used were as follows: anti-DHFR (1:400, Cell Signaling Technology), anti-ZEB2 (1:1000, Abcam), anti-E-cadherin (1:250, Abcam), anti-VIM (1:1500, Cell Signaling Technology), anti-ITGAV (1:1000, Cell Signaling Technology), anti-ITGB1 (1:2000, Cell Signaling Technology), anti-ITGB3 (1:2000, Cell Signaling Technology), anti-CD47 (1:2000, Abcam), and anti-β-actin (1:5000, Sigma-Aldrich).
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