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2 protocols using dipotassium hydrogen phosphate anhydrous

1

Quercetin-Iron(III) Chloride Assay Protocol

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Quercetin (purity ≥ 95%, Bangalore, Karnataka, India) and iron (III) chloride hexahydrate (purity ≥ 99%, Taufkirchen, Germany) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Dipotassium hydrogen phosphate anhydrous (purity ≥ 98%), potassium dihydrogen phosphate (purity ≥ 98%), sodium acetate anhydrous (purity ≥ 99%), hydrochloric acid (HCl, 37%), thiobarbituric acid (purity ≥ 98%), 1-butanol (purity ≥ 99.5%), and formic acid (FA, purity ≥ 98%) were purchased from PanReac AppliChem (Darmstadt, Germany). Furthermore, 2,4,4-tris(2-pyridyl)-1,3,5-triazine (TPTZ, purity ≥ 96%) was purchased from BLDpharm (Shanghai, China). Acetic acid (purity ≥ 99.8%) and ortho-phosphoric acid (purity ≥ 85%) were purchased from Component-Reaktiv (Moscow, Russia). Sodium thiosulfate standard titer was purchased from LLC Himtitry (Pereslavl-Zalessky, Russia). Starch indicator and sodium sulphate anhydrous were purchased from Himmed (Moscow, Russia). Acetic acid was purchased from Labtech (Moscow, Russia), and potassium iodide was purchased from Reahim (Moscow, Russia). Deionized water for chromatography (18 W) was obtained using a Milli-Q Merck water purification system (Millipore, Darmstadt, Germany).
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2

Antioxidant Activity Assays Analysis

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Fluorescein sodium salt (purity ≥ 97%); 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH, purity ≥ 97%); 5,5-dithiobis(2-nitrobenzoic acid) (DTNB, purity ≥ 98%); quercetin (purity ≥ 95%); iron (III) chloride hexahydrate (purity ≥ 99%); acetonitrile (for HPLC, gradient grade, ≥99.9%); hexane (for HPLC, ≥97.0%,GC); gallic acid (purity ≥ 97%); and tetramethylchromane-2-carboxylic acid (Trolox, purity ≥ 97%) were purchased from Sigma-Aldrich (USA). Di-potassium hydrogen phosphate anhydrous (purity ≥ 98%); potassium di-hydrogen phosphate (purity ≥ 98%); sodium acetate anhydrous (purity ≥ 99%); trichloroacetic acid (TCA; purity ≥ 99%); thiobarbituric acid (purity ≥ 98%); 1-butanol (purity ≥ 99.5%); pyrogallic acid (purity ≥ 99%); hydrogen peroxide (H2O2, purity ≥ 33%); sodium carbonate (purity ≥ 99.5%); Folin-Ciocalteu reagent; and hydrochloric acid (HCl; 37%) were purchased from PanReac AppliChem (Germany). Acetic acid (purity ≥ 99.8%); ortho-phosphoric acid (purity ≥ 85%) was purchased from Component-Reaktiv (Russia). 2,4,4-Tris(2-pyridyl)-1,3,5-triazine (TPTZ, purity ≥ 99%) was purchased from Acros Organics (China).
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