Epr quartz tubes

To record the CYP125A13 electron paramagnetic resonance (EPR) spectra, a Bruker X-band spectrometer (9.5 GHz) equipped with a continuous supply of helium cryostat ESR 900 and an ITC 4 temperature controller used to achieve a base temperature of 5 K, was used. The microwave frequency and modulation frequency used were 9.647GHz and 100 kHz, respectively. The data were recorded and analyzed at The Korea Basic Science Research Institute, Western Seoul Center. Before the measurement, the oxidized form of 180 μM CYP12A13 in 10 mM potassium phosphate buffer (pH 7.4) was frozen in liquid nitrogen in EPR quartz tubes (Wilmad). The spectral data comprising the g-tensor and line width of the heme centers were acquired by the accumulation of spectra in the Simfonia or Xsophe (Bruker) programs.

The Electron Paramagnetic Resonance (EPR) spectra were recorded on an X-Band Bruker Elexsys-II E500 CW-EPR spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany) at 90 ± 1 and 293 ± 1 K using a high sensitivity cavity (SHQE1119). Solid state EPR measurements were performed setting microwave frequency to 9 GHz, modulation frequency to 100 kHz, center field to 6000 G, sweep width to 12000 G, sweep time to 335.5 s, modulation amplitude to 20.37 G, microwave power to 15 mW, conversion time to 81.92 ms and resolution to 4096 points. The samples were put in EPR quartz tubes (Wilmad-LabGlass, Vineland, NJ, United States) and scanned three times, of which the average was used for analysis. The spectrum of an empty control tube was subtracted from all sample spectra. All spectra were analyzed with the Bruker Xepr software.

The Electron Paramagnetic Resonance (EPR) spectra were recorded as described earlier (11) at the University of Vienna on an X-Band Bruker Elexsys-II E500 CW-EPR spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany) at 90 ± 1 and 293 ± 1 K using a high sensitivity cavity (SHQE1119). Solid state EPR measurements were performed setting microwave frequency to 9 GHz, modulation frequency to 100 kHz, center field to 6000 G, sweep width to 12000 G, sweep time to 335.5 s, modulation amplitude to 20.37 G, microwave power to 15 mW, conversion time to 81.92 ms, and resolution to 4096 points. The samples were put in EPR quartz tubes (Wilmad-LabGlass, Vineland, NJ, United States) and scanned three times, of which the average was used for analysis. The spectrum of an empty control tube was subtracted from all sample spectra. All spectra were analyzed with the Bruker Xepr software.