Dihydrotestosterone dht

Manufactured by Cayman Chemical

Sourced in United States

Dihydrotestosterone (DHT) is a naturally occurring androgen hormone. It is the primary active metabolite of the hormone testosterone. DHT plays a crucial role in the development and maintenance of male sexual characteristics.

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Lab products found in correlation

Uk5099, by Merck Group (2 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Agilent Technologies (2 mentions) Oligomycin, by Agilent Technologies (2 mentions) Rotenone, by Agilent Technologies (2 mentions) Bptes, by Merck Group (2 mentions) R1881, by Merck Group (2 mentions) Isrib, by Merck Group (2 mentions) Azd3965, by Selleck Chemicals (2 mentions) Enzalutamide, by Selleck Chemicals (2 mentions) Du145, by Leibniz Institute DSMZ (1 mentions) 5 oxoete, by Cayman Chemical (1 mentions) 17β estradiol e2, by Merck Group (1 mentions) Restriction enzyme, by Takara Bio (1 mentions) Progesterone p4, by Merck Group (1 mentions) Fmoc chemistry, by GL Biochem (1 mentions)

4 protocols using dihydrotestosterone dht

Metabolic Modulator Evaluation Protocol

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Enzalutamide and AZD3965 were purchased from Selleck Chemicals.
Dihydrotestosterone (DHT) was purchased from Cayman Chemical. R1881, UK5099,
ISRIB, and BPTES were purchased from Sigma Aldrich. GW604714X was synthesized by
David Gooden at Duke University. Oligomycin, FCCP, and Rotenone were purchased
from Seahorse Biosciences. MSDC0160 was kindly provided under an MTA from Jerry
Colca at the Metabolic Solutions Development Company.

Bader D.A., Hartig S.M., Putluri V., Foley C., Hamilton M.P., Smith E.A., Saha P.K., Panigrahi A., Walker C., Zong L., Martini-Stoica H., Chen R., Rajapakshe K., Coarfa C., Sreekumar A., Mitsiades N., Bankson J.A., Ittmann M.M., O’Malley B.W., Putluri N, & McGuire S.E. (2018). Mitochondrial pyruvate import is a metabolic vulnerability in androgen receptor-driven prostate cancer. Nature metabolism, 1(1), 70-85.

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Cell Culture and Treatments

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THP-1 cells were kindly provided by Prof. Papakonstanti (School of Medicine, University of Crete, Heraklion, Greece). The DU-145 and T47D cell lines were purchased from DSMZ (Braunschweig, Germany). These cell lines and THP-1 differentiated macrophages were cultured in RPM1 medium supplemented with 10% FBS and 1% penicillin–streptomycin. White blood cells (WBCs) were cultured in DMEM, 4.5 g/L glucose medium supplemented with 10% FBS and 1% penicillin–streptomycin. Both primary cells and cell lines were cultured at 37 °C, 5% CO₂ in a humidified tissue culture incubator.
Unless otherwise stated, all media were purchased from Fisher Scientific (part of Thermo Fisher Scientific, Waltham, MA, USA), and all chemicals from Sigma (St. Louis, MO, USA). Cells were treated either with testosterone–BSA (testosterone carboxymethyl-oxime–bovine serum albumin) (10⁻⁶ M) (T3392), dihydrotestosterone (DHT) (10⁻⁶ M), 5-oxo-ETE (10⁻⁶ M), (Cayman Chemical Company, Ann Arbor, MI, USA), or Gue 1654 (10⁻⁶ M) (Tocris Bioscience, Bristol, UK) or their combinations. Appropriate vehicles were used as controls for each treatment.

Kalyvianaki K., Salampasi E.M., Katsoulieris E.N., Boukla E., Vogiatzoglou A.P., Notas G., Castanas E, & Kampa M. (2023). 5-Oxo-ETE/OXER1: A Link between Tumor Cells and Macrophages Leading to Regulation of Migration. Molecules, 29(1), 224.

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Metabolic Modulator Evaluation Protocol

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Avian Hormone Regulation Analysis

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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO), and restriction enzymes were purchased from Takara Biotechnology Co., Ltd., (Dalian, China). The recombinant human relaxin 3 (rhRLN3) was purchased from Roche R&D Center (China) Ltd. Chicken gonadotropin-releasing hormone (GnRH, GnRH1 was used in this study) and corticotropin-releasing hormone (CRH) were synthesized using solid-phase Fmoc chemistry (GL Biochem, Shanghai, China). 17β-estradiol (E2) and progesterone (P4) were purchased from Sigma-Aldrich. dihydrotestosterone (DHT) was purchased from Cayman Chemical Company (Ann Arbor, Michigan, United States). In this study, all chickens of the Lohmann Layer strain were purchased from a local commercial company (Chengdu, China). All primers used in this study were synthesized by Youkang Biological Technology Co., Ltd., (Hangzhou, China) and are listed in Table 1.

Lv C., Zheng H., Jiang B., Ren Q., Zhang J., Zhang X., Li J, & Wang Y. (2022). Characterization of relaxin 3 and its receptors in chicken: Evidence for relaxin 3 acting as a novel pituitary hormone. Frontiers in Physiology, 13, 1010851.

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