Cobalt protoporphyrin copp

Manufactured by Enzo Life Sciences

Sourced in United States

Cobalt protoporphyrin (CoPP) is a metalloporphyrin compound used as a research tool in cell biology and biochemistry laboratories. It is a cobalt-containing analog of the natural compound protoporphyrin IX. CoPP can be utilized in various experimental setups to study cellular processes, signaling pathways, and the effects of metal-containing porphyrins on biological systems.

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Lab products found in correlation

Sp600125, by AG Scientific (2 mentions) Ly294002, by AG Scientific (2 mentions) Pd98059, by AG Scientific (2 mentions) Sb203580, by AG Scientific (2 mentions) Crocin, by Merck Group (1 mentions) Kn 92, by Cayman Chemical (1 mentions) Kn 62, by Cayman Chemical (1 mentions) Actinomycin d actd, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Tata box binding protein tbp, by Santa Cruz Biotechnology (1 mentions) Lta from staphylococcus aureus, by Merck Group (1 mentions) Actinomycin d, by Merck Group (1 mentions)

3 protocols using cobalt protoporphyrin copp

Investigating Crocin and LPS-Induced Cellular Responses

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Crocin (Figure 1(a)), LPS (phenol extracted from Salmonella enteritidis), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), actinomycin D (Act. D), cycloheximide (CHX), 2-(3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl)benzoic acid acetoxymethyl ester (Fluo-3/AM), and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cobalt protoporphyrin (CoPP) and zinc protoporphyrin IX (ZnPP) were bought from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). LY294002, PD98059, SP600125, and SB203580 were obtained from A.G. Scientific (San Diego, CA, USA). Nifedipine, 1,2-bis (o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM), and calmidazolium chloride were acquired from Calbiochem (EMD Millipore, Billerica, MA, USA). KN92, KN93, and KN62 were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Calbiochem.

Kim J.H., Park G.Y., Bang S.Y., Park S.Y., Bae S.K, & Kim Y. (2014). Crocin Suppresses LPS-Stimulated Expression of Inducible Nitric Oxide Synthase by Upregulation of Heme Oxygenase-1 via Calcium/Calmodulin-Dependent Protein Kinase 4. Mediators of Inflammation, 2014, 728709.

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HEK293 Cell Culture and PAO Treatment

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Human embryonic kidney (HEK293) cells were purchased from the American Type Culture Collection (CRL-1573) and maintained in MEM medium (HyClone, Logan, UT) containing 10% fetal bovine serum (Sigma, St. Louis, MO), 1% antibiotic/antimycotic solution (Mediatech, Manassas, VA) at 37 °C in a 5% CO₂ incubator. PAO (Sigma) stock solution was freshly prepared for each exposure in 100% DMSO as described previously.^{14 (link)} PAO treatment of HEK293 cells was done employing various concentrations (0–250 nM) diluted in the culture medium. Vehicle (alone)-treated cells served as controls. Cobalt protoporphyrin (CoPP) (Enzo Life Sciences, Farmingdale, NY), an inducer of HO-1, was used in some experiments as described in the figure legends. In these experiments, cells were pretreated with CoPP (10 μM) for 6 h before PAO challenge and then both treatments were continued for 24 hours.

Srivastava R.K., Muzaffar S., Khan J., Traylor A.M., Zmijewski J.W., Curtis L.M., George J.F., Ahmad A., Antony V.B., Agarwal A, & Athar M. (2020). Protective role of HO-1 against acute kidney injury caused by cutaneous exposure to arsenicals. Annals of the New York Academy of Sciences, 1480(1), 155-169.

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Anti-inflammatory Effects of Dioscorea moniliforme Extract

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Cobalt protoporphyrin (CoPP) was purchased from Enzo Life Sciences, Inc. (USA). Actinomycin D (AD), cycloheximide (CHX), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and LTA from Staphylococcus aureus were purchased from Sigma-Aldrich Co. (USA). LY294002, PD98059, SP600125 and SB203580 were obtained from A.G. Scientific (USA). The antibodies for iNOS, HO-1, Nrf2, Akt, p38, TATA-box binding protein (TBP) and histone deacetylases (HDAC) were purchased from Santa Cruz Biotechnology (USA), the antibodies for phospho-Akt and phospho-p-38 were purchased from Cell Signaling Technology (USA), and the anti-tubulin antibody was purchased from Bio Genex (USA). The dry shoot systems of D. moniliforme Sw. were obtained and extracted as described previously [30] . In brief, the dry shoot systems (300 g) were extracted with distilled water at 100ºC for 4 h. The extract was filtered through a 0.45-μm filter, freeze-dried (yield: 8 g), and stored at 4ºC. The dried extract was dissolved in phosphate-buffered saline (PBS) and filtered through a 0.22 μm-filter before use.

Lee Y.J., Kim J.H, & Kim Y. (2018). Dendrobium moniliforme Stem Extract Inhibits Lipoteichoic Acid-Induced Inflammatory Responses by Upregulation of Heme Oxygenase-1. Journal of microbiology and biotechnology, 28(8).

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