Mastercycler realplex 2 equipment

Manufactured by Eppendorf

The Mastercycler realplex-2 is a real-time PCR thermal cycler manufactured by Eppendorf. It is designed to perform quantitative real-time PCR experiments. The device features a high-performance optical system and advanced temperature control for precise and reliable results.

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Lab products found in correlation

Rt2 first strand kit, by Qiagen (3 mentions) Rneasy plus mini kit, by Qiagen (2 mentions) Qpcr sybergreen master mix, by Qiagen (2 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Mastercycler (595 citations) Mastercycler Realplex (206 citations) RealPlex Mastercycler 2 (15 citations)

3 protocols using mastercycler realplex 2 equipment

qRT-PCR analysis of cell junctions

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Control and ShAkt1 cells were used for the qRT-PCR arrays. Briefly, cells were lysed and RNA was isolated using RNAase Mini plus Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. Next, cDNA was generated by RT2 First Strand Kit (SABiosciences, Frederick, MD), mixed with qPCR SyberGreen master mix and loaded into Tight-junction and Adherens-junction RT2 Profiler PCR Array plates. Reading was completed in Eppendorf Mastercycler realplex-2 equipment (Hauppauge, NY).

Gao F., Artham S., Sabbineni H., Al-Azayzih A., Peng X.D., Hay N., Adams R.H., Byzova T.V, & Somanath P.R. (2016). Akt1 promotes stimuli-induced endothelial-barrier protection through FoxO-mediated tight-junction protein turnover. Cellular and molecular life sciences : CMLS, 73(20), 3917-3933.

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Prostate Cancer Transcriptome Analysis

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Twenty five-week-old TRAMP/Akt1^−/− and TRAMP/Akt1^+/+ mice were treated with 10 mg/kg TCBN for 6 weeks, prostates were collected at 31 weeks and subjected to quantitative Real-Time PCR arrays. Briefly, tissues were lysed and RNA was isolated using RNeasy Mini Plus Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol. Next cDNA was generated using RT2 First Strand Kit (SA Biosciences, Frederick, MD), mixed with qPCR SyberGreen master mix and loaded into Mouse PCa RT2 Profiler PCR Array plate (SA Biosciences, Frederick, MD). Reading was performed in Eppendorf Mastercycler realplex-2 equipment (Eppendorf, Hauppauge, NY).

Gao F., Alwhaibi A., Sabbineni H., Verma A., Eldahshan W, & Somanath P.R. (2017). Suppression of Akt1- β-catenin pathway in advanced prostate cancer promotes TGFβ1-mediated epithelial to mesenchymal transition and metastasis. Cancer letters, 402, 177-189.

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Gene Array Analysis of EMT in HMECs

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Control and ShAkt1 HMEC lysates were used for the gene array analysis using human epithelial to mesenchymal transition gene arrays. Briefly, cells were lysed and RNA was isolated using RNeasy Mini plus Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions and the quality was confirmed by using Nanodrop 2000 spectrophotometer (Thermo Scientific). Next, cDNA was generated by RT2 First Strand Kit (SABiosciences, Frederick, MD), mixed with qPCR SyberGreen master mix and loaded into human EMT RT2 Profiler PCR Array plates. Reading was completed in Eppendorf Mastercycler realplex-2 equipment (Hauppauge, NY).

Sabbineni H., Verma A., Artham S., Anderson D., Amaka O., Liu F., Narayanan S.P, & Somanath P.R. (2019). Pharmacological inhibition of β-catenin prevents EndMT in vitro and vascular remodeling in vivo resulting from endothelial Akt1 suppression. Biochemical pharmacology, 164, 205-215.

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