6 aminofluorescein

Polycarbonate (PC, granular, 3 mm nominal size), (3-Aminopropyl)triethoxysilane (APTES), 99%, succinic anhydride, ≥99%, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), ≥98%, N-N-Diisopropylethylamine (EDIPA), 99.5%, MES hydrate, ≥99.5%, dodecylethyldimethylammonium bromide (DDAB), ≥98%, and 6-aminofluorescein, ≥95% were purchased from Sigma-Aldrich (St. Louis, MO, USA). PMSQ (Tospearl 120, dimeter of ~2 µm) were purchased from Momentive, Waterford, NY, USA. Silica nanoparticles (Aerosil OX 50, 40 nm, Evonik, Hanau, Germany). Methanol, ethanol, acetonitrile (ACN), toluene, and tetrahydrofuran (THF) of analytical grade with purity ≥99%, as well as ultra-pure deionized water (ULS/MS grade) were used as received without further purification. 2-(4-Morpholino)ethanesulfonic acid (MES) was purchased from Sigma-Aldrich Chemicals. Buffer 0.05 M was prepared by dissolving the appropriate amount of MES in deionized water.

All the materials were used without any further purification. 2,2,6,6-Tetramethyl-4-piperidyl methacrylate (>98%; PMA) was purchased from TCI Chemicals Pvt. Ltd. Glycidyl methacrylate (≥98%; GMA) and poly(ethyleneglycol)methylether 4-cyano 4[(dodecylsulfanylthiocarbonyl)sulfanyl] pentanoate (PEGmacro-CTA; M_n = 10 kDa) were purchased from Sigma. We measured M_p = 8300 g mol⁻¹ by SEC see Fig. 2 and M_n = 9.8 kg mol⁻¹ by NMR. 6-Aminofluorescein (≥95%; 6-AF), 3-chloroperbenzoic acid (≥78%; mCPBA) and 2,2′-azobis(2-methylpropionitrile) (≥98%; AIBN) were purchased from Sigma. Human LDL (Sigma-Aldrich) and Oil Red O solution (0.5% in isopropanol, Sigma-Aldrich) were purchased from Sigma. Hoechst 33342 Solution (20 mM) (Thermo Scientific™), ProLong® Gold mount media (Thermo Fisher Scientific), Nunc™ MicroWell™ 96-Well Microplates – Thermo Fisher Scientific, Total Antioxidant Capacity Assay Kit (Sigma-Aldrich), Cell Counting Kit – 8 (Sigma-Aldrich), Dulbecco's Phosphate Buffered Saline (PBS) (Cell culture grade, Sigma-Aldrich), DMEM (Dulbecco's Modified Eagle's medium) (Sigma-Aldrich), chloroform-d 99.8 atom % D (≥99.8%, Sigma-Aldrich), dichloromethane ≥99.8% stabilized.