High protein monkey diet

Four adult rhesus monkeys (Macaca mulatta), consisting of two males and two females, discriminated 0.032 mg/kg nicotine i.v. from saline. Monkeys were experimentally and pharmacologically naïve prior to the current study. Monkeys weighed 7.5-10.8 kg and were fed primate chow (Harlan Teklad High Protein Monkey Diet; Madison, WI), fresh fruit and peanuts. They were housed individually in stainless steel cages and maintained under controlled humidity and temperature on a 14/10-h light-dark cycle with continuous access to water in the home cage. The maintenance and experimental use of animals was carried out in accordance with the 2011 Guide for Care and Use of Laboratory Animals. All experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) at The University of Texas Health Science Center at San Antonio.

A total of 4 adult rhesus monkeys were used in these studies, two males (PE and BO), weighing between 8 and 11 kg, and two females (AM and RO), weighing between 8 and 10 kg. All monkeys were individually housed in an environmentally controlled vivarium under a 14h/10h light/dark cycle with free access to water in their home cage. Monkeys were fed primate chow (Harlan Teklad, High Protein Monkey Diet, Madison, WI), fresh fruit, and peanuts daily. Although experimental histories differed among these monkeys, all 4 monkeys had self-administered cocaine daily for the 3-4 years prior to completion of these studies. Information on estrous cycle was not recorded for these studies. All monkeys were maintained, and all experiments were performed, in accordance with the Institutional Animal Care and Use Committee, The University of Texas Health Science Center at San Antonio, and with the Guide for the Care and Use of Laboratory Animals (National Research Council 2011 ).

Five pharmacologically and experimentally naïve male adolescent rhesus monkeys (Macaca mulatta) were housed individually on a 14-h light/10-h dark schedule and maintained at 95% free-feeding weight (range 5.5–6.5 kg) with a diet consisting of primate chow (High Protein Monkey Diet; Harlan Teklad, Madison, WI), fresh fruit, and peanuts. Water was provided in the home cage. The Institutional Animal Care and Use Committee of The University of Texas Health Science Center at San Antonio approved the experimental protocols, and studies were conducted in accordance with the Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animal Resources, 2011 ).

Male rhesus monkeys (Macaca mulatta) were 7.1, 8.9, and 9.1 years old at the start of the experiment and weighed 8.5 – 10.0 kg. The monkeys were housed individually under a 14/10-h light/dark cycle. Monkeys had unrestricted access to water while in their home cages and were fed primate chow (Harlan Teklad, High Protein Monkey Diet, Madison, WI), fresh fruit, and peanuts daily after experimental sessions. The monkeys were maintained at a healthy body weight by food received in sessions and in the home cage. Monkeys were previously trained to press levers for food pellets and had received drug injections (Maguire et al., 2016 (link)). The monkeys had not received any drug for at least 2 months prior to the current study. The experimental protocol was approved by the Institutional Animal Care and Use Committee at the University of Texas Health Science Center at San Antonio and was in accordance with guidelines set forth by the Guide for the Care and Use of Laboratory Animals.

Four adult rhesus monkeys (Macaca mulatta), 2 females (SO and OL) and 2 males (GI and MO), were used in this study; SO and MO participated in a previous study (Maguire et al., 2013a ). Body weight (6-10 kg) was maintained by post-session feeding (Harlan Teklad, High Protein Monkey Diet, Madison, WI, USA). Monkeys received fresh fruit and peanuts daily and water was continuously available in home cages. Subjects were housed individually under a 14/10-h light/dark cycle with lights on at 06.00 h; experimental sessions started at 13.00 h and lasted approximately 100 min. Animals used in these studies were maintained in accordance with the Institutional Animal Care and Use Committee, The University of Texas Health Science Center at San Antonio, and the 2011 Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animals Resources on Life Sciences, National Research Council, National Academy of Sciences).

Eight adult rhesus monkeys contributed to these studies. The four females (monkeys DAI, DAH, GA, JA) weighed between 7.1 and 10.7 kg, and the four males (monkeys CH, HU, MU, KI) weighed between 9.5 and 12.3 kg. Four males participated in the choice procedure, precluding assessment of sex differences in either assay. Monkeys received sufficient quantities of primate chow (High Protein Monkey Diet; Harlan Teklad, Madison, WI), fresh fruit, and peanuts daily to maintain healthy weights, had unlimited access to water, and were housed individually in a room that was maintained under a 14-/10-hour light/dark cycle. These monkeys previously responded for drug under procedures similar to those described here (e.g., Gerak et al., 2019 (link); Weed et al., 2017 (link)). Monkeys were maintained in accordance with the Institutional Animal Care and Use Committee, The University of Texas Health Science Center at San Antonio, and the 2011 Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animal Resources on Life Sciences, National Research Council, National Academy of Sciences).

Two male and three female adult rhesus monkeys (Macaca mulatta) discriminated 1.78 mg/kg nicotine (s.c.) from saline. These monkeys had previously been trained to discriminate nicotine and had received both nicotinic and non-nicotinic drugs as described [16 (link), 19 (link)]. Monkeys weighed 9.0 (range: 6.7–12.5) kg and were fed primate chow (Harlan Teklad High Protein Monkey Diet; Madison, WI), fresh fruit and peanuts daily. Each monkey was housed individually in a stainless steel cage in a room with controlled temperature and humidity on a 14/10-h light-dark cycle. Water was available continuously in the home cages. The maintenance and experimental use of animals was carried out in accordance with the 2011 Guide for Care and Use of Laboratory Animals, and all experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) at The University of Texas Health Science Center at San Antonio.