Hyclone fbs

Manufactured by Thermo Fisher Scientific

Sourced in United States, Canada

HyClone FBS is a high-quality fetal bovine serum (FBS) product designed for cell culture applications. It is a cell culture supplement derived from the blood of bovine fetuses. HyClone FBS provides essential nutrients, growth factors, and other components that support the growth and maintenance of a wide variety of cell lines in vitro.

Automatically generated - may contain errors

Lab products found in correlation

61 protocols using hyclone fbs

Culturing Diverse Cell Lines for Research

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human 293F cells were maintained at 37° degrees Celsius with 5% CO₂ in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin. MS40L-low feeder cells (Mus musculus) were expanded from frozen aliquots in Iscove’s Modified Dulbecco’s Medium (Invitrogen) containing 10% HyClone FBS (Thermo scientific), 2-mercaptoethanol (5.5 × 10⁻⁵ M), penicillin (100 units/ml), and streptomycin (100 μg/ml; all Invitrogen) at 37° degrees Celsius with 5% CO₂ (Luo et al., 2009 (link); Su et al., 2016 (link)). Madin-Darby canine kidney cells (MDCK) (Canis lupus familiaris) were maintained in DMEM at 37° degrees Celsius with 5% CO₂. High Five™ Cells (BTI-TN-5B1–4) (Trichoplusia ni) were maintained at 28° degrees Celsius in EX-CELL 405 medium (Sigma) supplemented with penicillin and streptomycin. EL4 cells and EL4 cells transduced with influenza HAs were maintained at 37° C with 5% CO₂ in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated HyClone FBS (Fisher Scientific), HEPES buffer (10 mM), sodium pyruvate (1 mM), 1× MEM NEAA, 2-mercaptoethanol (5.5 × 10⁻⁵ M), penicillin (100 units/ml), and streptomycin (100 μg/ml; all Invitrogen). Cell lines were not subject to authentication.

Watanabe A., McCarthy K.R., Kuraoka M., Schmidt A.G., Adachi Y., Onodera T., Tonouchi K., Caradonna T.M., Bajic G., Song S., McGee C.E., Sempowski G.D., Feng F., Urick P., Kepler T.B., Takahashi Y., Harrison S.C, & Kelsoe G. (2019). Antibodies to a Conserved Influenza Head-interface Epitope Protect by an IgG Subtype Dependent Mechanism. Cell, 177(5), 1124-1135.e16.

+ Open protocol

+ Expand

CBX1 Variant N57D Characterization in LCLs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lymphoblastoid cell lines (LCLs) obtained from individual 1 with a CBX1 variant N57D and three gender-ethnicity matched control subjects were used. LCLs were cultured in RPMI 1640 with 300mg/L L-glutamine (Life Technologies, 11875085) supplemented with 20% HyClone FBS (Fisher Scientific, SH3007103), 0.2% penicillin-streptomycin (Life Technologies, 15140122), 0.2% Plasmocin (Invivogen, ant-mpp), and 1% Glutamax (Life Technologies, 35050061). HEK293T cell line was cultured in DMEM with 4.5 g/L D-Glucose and 110mg/L sodium pyruvate (Life Technologies, 11360070) supplemented with 10% HyClone FBS, and 0.2% penicillin-streptomycin. All cells were cultured at 37 °C in 5% CO2. CBX1 cDNA vectors were transfected to HEK293T cells using Lipofectamine 2000 (Life Technologies, 11668-030) according to the manufacturer's protocol.

Kuroda Y., Iwata‐Otsubo A., Dias K., Temple S.E., Nagao K., Hayr L.D., Zhu Y., Isobe S., Nishibuchi G., Fiordaliso S.K., Fujita Y., Ritter A., Baker S.W., Leung M.L., Koboldt D.C., Harman A., Keena B., Kazama I., Subramanian G.M., Manickam K., Schmalz B., Latsko M.S., Zackai E.H., Edwards M., Evans C., Dulik M.C., Buckley M.F., Yamashita T., O’Brien W.T., Harvey V.L., Obuse C., Roscioli T., & Izumi K. (2020). Dominant-negative mutations inCBX1cause a neurodevelopmental disorder.

+ Open protocol

+ Expand

Propagation of HaCaT and A375 Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

HaCaT^HRASG12V were provided by Ulrich Rodeck, PhD (Thomas Jefferson University) and propagated in CellGro DMEM F12 50/50 (Corning) supplemented with 10% HyClone FBS (Invitrogen), CellGro glutamine (Corning), Primocin (Invivogen), and 40μg/ml G418 (Santa Cruz Biotech). A375 was obtained from Suhendan Ekmekcioglu, PhD (MDACC) and were propagated in HyClone RPMI-1640 (Invitrogen) supplemented with 5% HyClone FBS (Invitrogen), CellGro glutamine (Corning), and pen/strep (Corning). All lines were authenticated via the MDACC internal STR profiling service and matched to existing databases.

Adelmann C.H., Ching G., Du L., Saporito R.C., Bansal V., Pence L.J., Liang R., Lee W, & Tsai K.Y. (2016). Comparative profiles of BRAF inhibitors: the paradox index as a predictor of clinical toxicity. Oncotarget, 7(21), 30453-30460.

+ Open protocol

+ Expand

Differentiation of Single B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

On day −1, NB-21.2D9 cells were seeded into 96-well plates at 1,000 cells/well in B-cell media (BCM); RPMI-1640 medium (Invitrogen) supplemented with 10% HyClone FBS (Thermo scientific), 5.5 × 10⁻⁵ M 2-mercaptoethanol, 10 mM HEPES, 1 mM sodiumpyruvate, 100 units/ml penicillin, 100 μg/ml streptomycin, and 1 × MEM nonessential amino acid (all Invitrogen). Next day (day 0), recombinant mouse IL-4 (Peprotech; final 2 ng/ml) was added to the cultures, and then single B cells were directly sorted into each well using cell sorters [FACSVantage or FACSAria (both BD Biosciences)]. On day 2, 50% (vol.) of the culture media were removed from cultures and 100% (vol.) of fresh BCM were added to the cultures. On days 3 to 8, two-thirds of the culture media were replaced with fresh BCM every day. On day 9, culture supernatants were harvested for ELISA determinations and culture plates were stored at −80°C for V(D)J amplifications.

Nojima T., Reynolds A.E., Kitamura D., Kelsoe G, & Kuraoka M. (2020). Tracing self-reactive B cells in normal mice. Journal of immunology (Baltimore, Md. : 1950), 205(1), 90-101.

+ Open protocol

+ Expand

Culturing HepG2 Hepatic Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HepG2 human hepatic cell line (HB-8065™) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were cultured in RPMI 1640 (Gibco™, Thermo Fisher Scientific) supplemented with 10% Hyclone™ FBS (ThermoFisher Scientific), and penicillin/streptomycin (100 U/mL and 100 μg/mL, respectively). Cells were incubated in a humidified incubator at 37°C and 5% CO₂. Cells were passaged twice a week, keeping the confluence below 80%. Cells were used between passages 20 to 40.

Pisani C., Rascol E., Dorandeu C., Gaillard J.C., Charnay C., Guari Y., Chopineau J., Armengaud J., Devoisselle J.M, & Prat O. (2017). The species origin of the serum in the culture medium influences the in vitro toxicity of silica nanoparticles to HepG2 cells. PLoS ONE, 12(8), e0182906.

+ Open protocol

+ Expand

Single-cell Memory B Cell Culture

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

All culture supernatants from single-cell cultured memory B cells used in this study were generated and reported as part as a previous study (see Sokal et al., 2021b (link)). Briefly, single B cells were sorted in 96-well plates containing MS40L^lo cells expressing CD40L (kind gift from G. Kelsoe, Crickx et al., 2021 (link); Luo et al., 2009 (link)). Cells were co-cultured at 37°C with 5% CO2 during 21 or 25 days in RPMI-1640 (Invitrogen) supplemented with 10% HyClone FBS (Thermo Scientific), 55 μM 2-mercaptoethanol, 10 mM HEPES, 1 mM sodium pyruvate, 100 units/mL penicillin, 100 μg/mL streptomycin, and MEM non-essential amino acids (all Invitrogen), with the addition of recombinant human BAFF (10 ng/ml), IL2 (50 ng/ml), IL4 (10 ng/ml), and IL21 (10 ng/ml; all Peprotech). Part of the supernatant was carefully removed at days 4, 8, 12, 15 and 18 and the same amount of fresh medium with cytokines was added to the cultures. After 21 days of single cell culture, supernatants were harvested and stored at -20°C. Cell pellets were placed on ice and gently washed with PBS (Gibco) before being resuspended in 50 μL of RLT buffer (Qiagen) supplemented with 1% 2-mercaptoethanol and subsequently stored at -80°C until further processing.

Sokal A., Broketa M., Barba-Spaeth G., Meola A., Fernández I., Fourati S., Azzaoui I., de La Selle A., Vandenberghe A., Roeser A., Bouvier-Alias M., Crickx E., Languille L., Michel M., Godeau B., Gallien S., Melica G., Nguyen Y., Zarrouk V., Canoui-Poitrine F., Noizat-Pirenne F., Megret J., Pawlotsky J.M., Fillatreau S., Simon-Lorière E., Weill J.C., Reynaud C.A., Rey F.A., Bruhns P., Chappert P, & Mahévas M. (2022). Analysis of mRNA vaccination-elicited RBD-specific memory B cells reveals strong but incomplete immune escape of the SARS-CoV-2 Omicron variant. Immunity, 55(6), 1096-1104.e4.

+ Open protocol

+ Expand

Ifnar1 Knockout Mouse Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four to eight-week-old male and female C57BL/6J (WT) and Ifnar1^-/- mice were bred in a barrier facility at Washington University School of Medicine. All animal studies were approved and performed in accordance with the Washington University School of Medicine Institutional Animal Care and Use Committees. Vero cells (ATCC) were grown in Dulbecco’s Modified Eagle Medium (DMEM) media (Corning Cellgro) supplemented with 10% Fetal Bovine Serum (FBS, Biowest), 100 U/mL Penicillin (Life Technologies), 100 μg/mL streptomycin (Life Technologies), and 2 mM L-Glutamine (Corning). 293T cells (gift from Dr. Webby at St. Jude Children’s Research Hospital) were maintained in Opti-MEM (Life Technologies) with 10% Hyclone FBS (Thermo Fisher Scientific), 2 mM L-Glutamine, 100 U/ml of Penicillin and 10 μg/mL of streptomycin.

Bricker T.L., Shafiuddin M., Gounder A.P., Janowski A.B., Zhao G., Williams G.D., Jagger B.W., Diamond M.S., Bailey T., Kwon J.H., Wang D, & Boon A.C. (2019). Therapeutic efficacy of favipiravir against Bourbon virus in mice. PLoS Pathogens, 15(6), e1007790.

+ Open protocol

+ Expand

Single B Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single B cells were cultured in the presence of NB-21.2D9 feeder cells. NB-21.2D9 cells were seeded into 96-well plates at 1,000 cells/well in B cell media (BCM); RPMI-1640 (Invitrogen) supplemented with 10% HyClone FBS (Thermo scientific), 5.5 × 10⁻⁵ M 2-mercaptoethanol, 10 mM HEPES, 1 mM sodium pyruvate, 100 units/ml penicillin, 100 μg/ml streptomycin, and MEM nonessential amino acid (all Invitrogen). Next day (Day 0), recombinant mouse IL-4 (Peprotech; 2 ng/ml) was added to the cultures, and then single B cells were directly sorted into each well using a FACS Vantage. On day 2, 50% (vol.) of culture media were removed from cultures and 100% (vol.) of fresh BCM were added to the cultures. On days 3 to 8, two-thirds of the culture media were replaced with fresh BCM every day. On day 9 or 10, culture supernatants were harvested for ELISA determinations and culture plates were stored at −80°C for V(D)J amplifications.

Kuraoka M., Schmidt A.G., Nojima T., Feng F., Watanabe A., Kitamura D., Harrison S.C., Kepler T.B, & Kelsoe G. (2016). Complex Antigens Elicit Diverse Patterns of Clonal Selection in Germinal Centers. Immunity, 44(3), 542-552.

+ Open protocol

+ Expand

Single B Cell Culture Conditions

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell culture was performed as previously described (Crickx et al., 2021 (link)). Single B cells were sorted in 96-well plates containing MS40L^lo cells expressing CD40L (kind gift from G. Kelsoe; Luo et al., 2009 (link)). Cells were co-cultured at 37°C with 5% CO2 during 21 or 25 days in RPMI-1640 (Invitrogen) supplemented with 10% HyClone FBS (Thermo Scientific), 55 μM 2-mercaptoethanol, 10 mM HEPES, 1 mM sodium pyruvate, 100 units/mL penicillin, 100 μg/mL streptomycin, and MEM non-essential amino acids (all Invitrogen), with the addition of recombinant human BAFF (10 ng/ml), IL2 (50 ng/ml), IL4 (10 ng/ml), and IL21 (10 ng/ml; all Peprotech). Part of the supernatant was carefully removed at days 4, 8, 12, 15 and 18 and the same amount of fresh medium with cytokines was added to the cultures. After 21 days of single cell culture, supernatants were harvested and stored at −20°C. Cell pellets were placed on ice and gently washed with PBS (GIBCO) before being resuspended in 50 μL of RLT buffer (QIAGEN) supplemented with 1% 2-mercaptoethanol and subsequently stored at −80°C until further processing.

Sokal A., Barba-Spaeth G., Fernández I., Broketa M., Azzaoui I., de La Selle A., Vandenberghe A., Fourati S., Roeser A., Meola A., Bouvier-Alias M., Crickx E., Languille L., Michel M., Godeau B., Gallien S., Melica G., Nguyen Y., Zarrouk V., Canoui-Poitrine F., Pirenne F., Mégret J., Pawlotsky J.M., Fillatreau S., Bruhns P., Rey F.A., Weill J.C., Reynaud C.A., Chappert P, & Mahévas M. (2021). mRNA vaccination of naive and COVID-19-recovered individuals elicits potent memory B cells that recognize SARS-CoV-2 variants. Immunity, 54(12), 2893-2907.e5.

+ Open protocol

+ Expand

HEK293T Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T cells (ATCC # CRL-11268) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco-Invitrogen), supplemented with 0.37% sodium bicarbonate, 100 U/ml penicillin-streptomycin (Thermo Fisher Scientific), and fetal bovine serum (HyClone FBS, Thermo Fisher Scientific). Cells were maintained in DMEM with 5% FBS.

Haywood E.E., Handy N.B., Lopez J.W., Ho M, & Wilson B.A. (2021). Insertion-trigger residues differentially modulate endosomal escape by cytotoxic necrotizing factor toxins. The Journal of Biological Chemistry, 297(5), 101347.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!