3 4 5 dimethylthazol 2 yl 2 5 diphenyltetrazolium bromide

α-D-Glucose 1,6-bisphosphate potassium salt hydrate, α-D-Glucose 1-phosphate disodium salt hydrate, α-D(+)Mannose 1-phosphate sodium salt hydrate, Inosine 5′-monophosphate disodium salt hydrate, Adenosine 5′-triphosphate disodium salt hydrate, Maltose phosphorylase from Enterococcus sp., Fructose-6-phosphate Kinase from Bacillus stearothermophilus, Trimethylamine, 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide were purchased from Sigma-Aldrich (Sigma-Aldrich, Milan, Italy). Trypsin was purchased from ICN Pharmaceuticals (MP Biomedicals Germany GmbH).
AG1x8 Resin 200-400 Mesh Hydroxide Form was purchased from Bio-Rad (Bio-Rad Laboratories Srl, Milan, Italy).
Sypro Orange was from Invitrogen Molecular Probes (Invitrogen Molecular Probes, Monza, Italy), StepOne^TM Real Time PCR System from Applied Biosystems (Applied Biosystems, Foster City, CA, USA), strips from Sarstedt (Multiply–μStrip Pro 8-strip low profile) (Sarstedt Srl, Milan, Italy).
D(+)Maltose monohydrate, β-Nicotinamide adenine dinucleotide phosphate sodium salt, Glucose-6-phosphate Dehydrogenase, Creatine phosphate disodium salt thetrahydrate were from Alfa Aesar (Alfa Aesar, Thermo Scientific, Kandel, Germany).

Pitolisant, 3-(4-chlorophenyl)propyl 3-piperidinopropyl ether as hydrochloride salt, was synthesized in the Department of Technology and Biotechnology of Drugs, Jagiellonian University Medical College in Kraków (Kraków, Poland).
Astrocyte medium, astrocyte growth supplement, fetal bovine serum, penicillin/streptomycin solution and poly-L-Lysine were from ScienCell Research Laboratories (Carlsbad, CA, USA).
The 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), clorgyline (N-methyl-N-propargyl-3-(2,4-dichlorophenoxy)propylamine hydrochloride), deprenyl (R-(-)-deprenyl hydrochloride) and dimethyl sulfoxide were purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA).
The reagents for radioassays, i.e., β-phenylethylamine hydrochloride [ethyl-1-¹⁴C], hydroxytryptamine binoxalate (serotonin binoxalate), 5-[2-¹⁴C] and adenosyl-L-methionine, S-[methyl-¹⁴C] were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA).