Klrg1 fitc

Manufactured by Thermo Fisher Scientific

KLRG1-FITC is a fluorescently-labeled antibody used for the detection and analysis of KLRG1-expressing cells in flow cytometry applications.

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Close spelling variants of this product

KLRG1 (14 citations) FITC-conjugated anti-KLRG1 (clone 2F1; (3 citations) Anti-KLRG1 FITC (2 citations)

3 protocols using klrg1 fitc

Multiparameter Flow Cytometry of Immune Cells

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CD45.2-V500 and TNFα-APC-Cy7 were purchased from BD Biosciences (San Jose, California). KLRG1-FITC, Eomes-PE, CD107a-PE, CD107b-PE, CD27-PE, CD127-PE-Cy5, CD127-PerCP-Cy5.5, Tbet-PerCP-Cy5.5, IFNγ-PerCP-Cy5.5, Eomes-PErCP-efluor710, CD45.1-PECy7, KLRG1-PE-Cy7, Tbet-PE-Cy7, IRF4-AlexaFluor647, CD44-AlexaFluor700, CD62L-APC-eFluor780, CD44-eFluor450, KLRG1-eFluor450, IFNγ-eFluor450, CD90.2-APC-eFluor780, CD45.1-APC-eFluor780, IL-2-PerCP-Cy5.5 were purchased from eBioscience (San Deigo, California). CD8-PE-TexasRed, GranzymeB-PE, GranzymeB-APC, Live-Dead-Violet, Live-Dead-Aqua and goat-anti-rabbit IgG-AlexaFluor647 and -AlexaFluor488 were purchased from Life Technologies (Grand Island, New York). H2D^b-GP33 monomers were prepared at UMMS; LCMV-specific (H2D^b-NP396, H2D^b-GP276) and Influenza A PR8-OVA_I-specific (H2K^b-OVA257) monomers were obtained from the NIH Tetramer Core Facility (Atlanta, Georgia). Intracellular TCF1 staining was performed using rabbit-anti-mouse TCF1 (Cell Signaling Technology, Danvers, Massachusetts) followed by staining with goat-anti-rabbit secondary (Life Technologies). Samples were analyzed on an LSRII flow cytometer (Becton Dickinson), and data were analyzed using FlowJo (Tree Star).

Nayar R., Schutten E., Bautista B., Daniels K., Prince A.L., Enos M., Brehm M.A., Swain S.L., Welsh R.M, & Berg L.J. (2014). Graded levels of IRF4 regulate CD8+ T cell differentiation and expansion, but not attrition, in response to acute virus infection. Journal of immunology (Baltimore, Md. : 1950), 192(12), 5881-5893.

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Activation of T-cell Signaling Pathways

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Antibodies against SKAP55 (cat#611236) (BD Pharmingen), ADAP (cat#07-546) (Upstate Biotechnology), NAFTc1 (cat#sc-7294) (Santa Cruz Biotechnology), Fyn (cat#BS2739) (Bioworld Technology), and anti-human CD28 (cat# 348040) (BD Pharmingen) were purchased as indicated. Anti-human CD3 (OKT3) (cat# BE0001-1), anti-mouse CD3 (2C11) (cat#BE0001-1), and anti-mouse CD28 (PV1) (cat#BE0015-5) were from Bio X cell (West Lebanon, NH). Anti-mouse PD-1 PE (cat#12-9981), CTLA4 PE (cat#12-1522), Va2 TCR FITC (cat#11-5812), Vβ5 TCR PerCP-eFluor710 (cat#46-5796), CD44 FITC (cat#11-0441), CD4 PE-Cy7 (cat#25-0041), CD11c FITC (cat#11-0114), CD8a APC (cat#17-0081), CD127 PE (cat#12-1271), F4/80 APC (cat#17-4801), Foxp3 APC (cat#17-5773), Granzyme B FITC (cat#11-8898), and KLRG1 FITC (cat#11-5893) were from eBioscience. Anti-PD-1 (Clone J43) (BE0033-2**) and isotype IgG (BE0091) used in animal experiments were purchased from Bio X cell (West Lebanon, NH). GM-CSF (Peprotech), LPS (lipopolysaccharide, SIGMA), and OVA_257-264 peptide (SIINFEKL, AnaSpec) were ordered.

Li C., Li W., Xiao J., Jiao S., Teng F., Xue S., Zhang C., Sheng C., Leng Q., Rudd C.E., Wei B, & Wang H. (2015). ADAP and SKAP55 deficiency suppresses PD-1 expression in CD8+ cytotoxic T lymphocytes for enhanced anti-tumor immunotherapy. EMBO Molecular Medicine, 7(6), 754-769.

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Multiparametric Flow Cytometry Analysis

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The following antibodies were purchased from BD: Ki67 AF700, CD4 Pacific Blue, CD8α PE-CF594, CD3 PE-CF594, CD4 BV650, and CD11a BUV805. The following antibodies were purchased from eBioscience: KLRG-1 FITC, Ly6C (clone HK1.4) PerCP-Cy5.5, CD69 eFluor450, CD69 FITC, CD69 PE-Cy7, T-bet eFluor660, CD45.2 APC-eFluor780, and CD3 APC-eFluor780. The following antibodies were purchased from BioLegend: LFA-1 PerCP-Cy5.5, CD3 Pacific Blue, CD3 BV785, CD11a PE, and CD11a PErCP-Cy5.5. Invitrogen live/dead Aqua stain or Tonbo Ghostdye510 was used to determine viability. PE-conjugated Tgd-057 MHC-I tetramers and PE-conjugated AS-15 MHC-II tetramers were provided by NIH Tetramer Core. All samples were run on an LSRFortessa (BD), Canto (BD), or FACSymphony A3 and analyzed using FlowJo software (Tree Star). Images were obtained using the ImageStream (Amnis) and analyzed using IDEAS software (Amnis). To determine T-bet localization, nuclear and cytoplasmic masking functions were made using DAPI staining; these masks were then applied to T-bet expression.

Pritchard G.H., Phan A.T., Christian D.A., Blain T.J., Fang Q., Johnson J., Roy N.H., Shallberg L., Kedl R.M, & Hunter C.A. (2022). Early T-bet promotes LFA1 upregulation required for CD8+ effector and memory T cell development. The Journal of Experimental Medicine, 220(2), e20191287.

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