Human cd34 microbeads kit

Manufactured by Miltenyi Biotec

Sourced in United States

The Human CD34 MicroBeads Kit is a laboratory product designed for the magnetic separation and isolation of CD34-positive cells from various biological samples. It contains magnetic beads coated with antibodies specific to the CD34 antigen, a cell surface marker commonly expressed on hematopoietic stem and progenitor cells.

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Lab products found in correlation

Ficoll hystopaque plus, by GE Healthcare (2 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions) Mv4 11, by American Type Culture Collection (1 mentions) Cc100 cytokine cocktail, by STEMCELL (1 mentions) Gm csf, by Thermo Fisher Scientific (1 mentions) Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD34 MicroBead Kit (230 citations) Human CD34 MicroBead Kit (73 citations) CD34 microbeads (53 citations) CD34 MicroBeads kit (14 citations) CD34 MicroBead Kit UltraPure human (14 citations) MicroBead kits (12 citations) Human CD34 MicroBeads (9 citations) MACS human CD34 MicroBead kit (2 citations) Human anti-CD34 MicroBeads Isolation Kit (2 citations)

3 protocols using human cd34 microbeads kit

Culturing AML Cell Lines and Patient Samples

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AML cell lines (KG1A, MV411 and NB4; ATCC) were cultured in IMDM (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (FBS; Atlanta Biologicals, Flowery Branch, GA, USA). AML patient and control samples (peripheral blood or BM) were obtained under a City of Hope-Institutional Review Board—approved protocol with informed consent from patients or healthy individuals (see Supplementary Table 1 for more information). BM CD34+ cells were enriched using human CD34+ microbeads kit (Miltenyi Biotec, Auburn, CA, USA).

Kumar B., Garcia M., Weng L., Jung X., Murakami J.L., Hu X., McDonald T., Lin A., Kumar A.R., DiGiusto D.L., Stein A.S., Pullarkat V.A., Hui S.K., Carlesso N., Kuo Y.H., Bhatia R., Marcucci G, & Chen C.C. (2017). Acute myeloid leukemia transforms the bone marrow niche into a leukemia-permissive microenvironment through exosome secretion. Leukemia, 32(3), 575-587.

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CD34+ Cell Isolation and Differentiation

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Bone marrow (BM)-derived CD34+ were obtained from Calabretta’s lab at Thomas Jefferson University. CD34+ CD33− cells were sorted at the Flow Cytometry facility at Wistar Institute. Fetal liver (FL)-derived CD34+ cells were obtained from Stem Cell and Xenograft Core at University of Pennsylvania and were maintained in StemSpan SFEM medium supplemented with CC100 cytokine cocktail (Stem Cell Technologies). De-identified human cord blood (CB) was obtained from volunteers with informed consent at Helen F. Graham Cancer Center and Research Institute at Christiana Hospital. Mononucleated cells (MNC) were separated with Ficoll-Hystopaque Plus (GE Healthcare). CD34+ cells were then isolated using human CD34 MicroBeads Kit (Miltenyi Biotec) following manufacturer’s instructions. CD34+ were maintained in StemSpan SFEM medium supplemented with 1X CC100 cytokine cocktail. BM- and FL-derived CD34+ were used for CFU assay, BM-, FL- and CB-derived CD34+ were in vitro differentiated and used for RNA-seq and ChIP-seq experiments, respectively. Circulating monocytes were obtained from the Human Immunology Core at University of Pennsylvania.

Barbieri E., Trizzino M., Welsh S.A., Owens T.A., Calabretta B., Carroll M., Sarma K, & Gardini A. (2018). Targeted enhancer activation by a subunit of the Integrator complex. Molecular cell, 71(1), 103-116.e7.

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CD34+ Cells Differentiation into Monocytes

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Deidentified human cord blood was obtained from volunteers with informed consent at Helen F. Graham Cancer Center and Research Institute at Christiana Hospital. The protocol was approved by the ChristianaCare Institutional Review Board. Mononucleated cells were separated with Ficoll-Hystopaque Plus (GE Healthcare). CD34⁺ cells were then isolated using the human CD34 MicroBeads Kit (Miltenyi Biotec) following the manufacturer’s instructions. CD34⁺ cells were differentiated into monocytes in serum-free expansion medium (SFEM) supplemented with stem cell factor (SCF; 100 ng/ml), IL-3 (10 ng/ml), M-SCF (50 ng/ml), and GM-CSF (25 ng/ml; PeproTech).

Trizzino M., Zucco A., Deliard S., Wang F., Barbieri E., Veglia F., Gabrilovich D, & Gardini A. (2021). EGR1 is a gatekeeper of inflammatory enhancers in human macrophages. Science Advances, 7(3), eaaz8836.

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