Cell proliferation was measured every 24 hours for four days, starting one day after transfection using either CellTiter-FluorTM cell viability assay (Promega) or crystal violet staining followed by dye extraction using methanol and optical density measured at 570 nm [55 ]. Briefly, 5×103 cells/well transfected with STAG1, STAG2 or negative siRNAs were seeded on 96-well plates in a final volume of 100 μl per well. For the CellTiter-FluorTM cell viability assay (Promega), at each time point, 20 μl of the diluted reagent (10 μl of the GF-AFC Substrate in 2 ml of Assay Buffer) was added to each well. After one hour and 30 minutes, fluorescence was measured at 380–400 nmEx/505Em using a Fusion alpha-FP (Perkin Elmer). Each condition was assessed in triplicate and the whole experiment was repeated at least twice. Crystal violet staining was used to visualise the effect of STAG1 and STAG2 KD on CAL-51 cells. Briefly, 70000 cells were seeded on 12-well plates and transfected with the universal negative control, STAG1, STAG2 or STAG1 and STAG2 siRNAs. After five days, cells were fixed with ice-cold 100% methanol and stained with 0.1% crystal violet. After 30 minutes, cells were washed three times with water and dried.
Celltiter fluor cell viability assay
Manufactured by Promega
Sourced in United States
The CellTiter-Fluor Cell Viability Assay is a fluorescence-based cell viability assay. It measures the relative number of viable cells in a sample by detecting a protease activity marker.
Automatically generated - may contain errors
Lab products found in correlation
One glo luciferase assay, by Promega (3 mentions)
Spectramax m5, by Molecular Devices (3 mentions)
Ly2584702, by Selleck Chemicals (2 mentions)
4egi 1, by Selleck Chemicals (2 mentions)
Azd8055, by Selleck Chemicals (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Arvo mx, by PerkinElmer (2 mentions)
Caspase glo 3 7 assay, by Promega (2 mentions)
Infinite m1000, by Tecan (2 mentions)
Fusion alpha fp, by PerkinElmer (1 mentions)
Celltiter glo assay, by Promega (1 mentions)
Prism 8, by GraphPad (1 mentions)
Caspase glo 3 7 assay system, by Promega (1 mentions)
96 well tissue culture treated plates, by Greiner (1 mentions)
Magellan data analysis software, by Tecan (1 mentions)
96 well plate, by Greiner (1 mentions)
Spectramax m2e, by Molecular Devices (1 mentions)
Agilent bravo, by Agilent Technologies (1 mentions)
Celltiter 96 proliferation assay mts, by Promega (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
58 protocols using celltiter fluor cell viability assay
1
Measuring Cell Proliferation with STAG1 and STAG2 KD
2
Caspase Inhibition Enhances Cell Survival
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IBC cell lines, SUM149PT and SUM190PT, were seeded in 96-well plates and treated with and without Salubrinal in the absence or presence of specific, irreversible caspase inhibitors (caspase-2, -3, -6, -8, -9, and -10) from R&D systems. Enhanced cell survival was determined using the CellTiter-fluor cell viability assay from Promega (#G6080) per the manufacturer’s instructions. This assay measures a conserved and constitutive protease activity within live cells using a fluorogenic substrate. Loss of cell membrane integrity inactivates this protease.
3
Cell Viability and Luciferase Assay
4
Quantifying Tumor Cell Viability and Killing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For cell viability measurements, cells were cultured in 96-well plates and metabolic activity was measured using Cell-Titer-Glo assay or CellTiter-Fluor Cell Viability Assay (Promega), as per the manufacturer’s protocol, in GloMax plate reader. Killing assay was performed in 96-well plates. For killing assay, 33F8 cells expressing Luciferase were seeded in 96-well plates, and 33F8 TILs were added to them at varying ratios, in the presence or absence of various Chk1 inhibitors. Luciferase assay was carried out 24 and 48 h posttreatment to assess viability of the tumor cells.
5
Caspase-Mediated Virus Inhibition Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Caspase activity was measured
using a luciferase reporter. Briefly, Vero cells were seeded at a
density of 1 × 104 cells/well in 96-well tissue culture
treated plates (Greiner). The next day, the medium was removed and
the cells were infected with virus that had been pretreated with selected
compounds or controls at 37 °C for 1 h. Plates were incubated
for 3 days. Cell viability was first measured using the CellTiter-Fluor
Cell Viability Assay (Promega), and then caspase activity was measured
using the Caspase-Glo 3/7 Assay System (Promega) according to the
manufacturer’s protocol using a Spectra Max M5 plate reader
(Molecular Devices). All experiments were performed in triplicate.
Additionally, for the dose–response assay, the 50% inhibitory
concentration (IC50) was computed and plotted using Prism
8.4 (GraphPad Software, La Jolla, CA).
using a luciferase reporter. Briefly, Vero cells were seeded at a
density of 1 × 104 cells/well in 96-well tissue culture
treated plates (Greiner). The next day, the medium was removed and
the cells were infected with virus that had been pretreated with selected
compounds or controls at 37 °C for 1 h. Plates were incubated
for 3 days. Cell viability was first measured using the CellTiter-Fluor
Cell Viability Assay (Promega), and then caspase activity was measured
using the Caspase-Glo 3/7 Assay System (Promega) according to the
manufacturer’s protocol using a Spectra Max M5 plate reader
(Molecular Devices). All experiments were performed in triplicate.
Additionally, for the dose–response assay, the 50% inhibitory
concentration (IC50) was computed and plotted using Prism
8.4 (GraphPad Software, La Jolla, CA).
6
Cell Viability Assay for EA and MDSA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The relative number of viable cells in a population was determined using the CellTiter-Fluor™ Cell Viability Assay (G6080, Promega, Madison, WI, USA). On 96 well plates, 1 × 104 cells were seeded and incubated for 24 h in 100 µL medium. For an additional 24 h, the cells were treated with various concentrations of EA and MDSA. After replacing 50 µL medium with 50 µL assay reagent in each well, the cells were incubated for 30 min at 37 °C with 5% CO2. The viability of the cells was determined using the Biotek® multi-mode microplate reader set to Ex/Em=490/505 nm (Agilent, Santa Clara, CA, USA).
7
Cell Viability Assay Protocol
8
Quantifying BeWo Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Relative BeWo cell numbers were assessed using the CellTiter-Fluor Cell Viability Assay (Promega). In 96-well plates (Greiner bio-one), cells were seeded at 10,000 cells/well and treated the following day with drugs as described above. Cell number was measured at 48 hours of culture according to the manufacturer's protocol. The plate was read using the spectrometer and recorded OD values were blanked using the media-only control to account for background fluorescence. Fluorescent measurements obtained with this assay represented an indication of the relative BeWo cell number, such that cell proliferation could be assessed between different drug treatments relative to their respective controls (set as 1).
9
Cell Proliferation Assay of 1-3
10
Cell Viability Assay of GH and Phen Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T98G cells (3000 cells/well) were seeded in 384-well tissue culture treated black-walled, clear-bottom microplates (Corning Life Sciences, Tewksbury, MA, USA). After 24 h, cells were treated with either solvents (PBS or DMSO) control, GH, Phen, or the combination of GH and Phen using an Agilent Bravo automated liquid handling system (Agilent Technologies, Santa Clara, CA, USA). CellTiter-Fluor™ Cell Viability Assay (Promega, Madison, WI, USA, Cat.# G6080) was performed following manufacturer protocol to measure live-cell protease activity in cells treated from day 0 to day 3. Briefly, cells were treated with drugs in 25 µL and, at indicated time points, an equal volume of GF-AFC substrate was added. The cleaved compound generates a fluorescence signal proportional to the number of living cells. After 30 min of incubation at 37 °C, the signal was measured using a Spectramax M2e microplate reader (380–400 nm Ex/505 nm Em) (Molecular Devices, San Jose, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!