LH in the blood serum was detecting using Sheep Luteinizing Hormone (LH) ELISA Kit (CUSABIO, Wuhan, China) with detection reange 2-75 mIU/mL, and intra-assay and inter-assay coe cient of variations (CVs) were both ≤ 15%, according to the instruction. Testosterone and estradiol were detected by using an automated chemiluminescent microparticle immunoassay with Architect 2nd generation testosterone kit and Architect estradiol assay kit on Architect i4000SR system (Abbott Park, USA) as we previously mentioned [13] .
Architect i4000sr system
Manufactured by Abbott
Sourced in United States
The Architect i4000SR System is a fully automated chemistry analyzer designed for high-throughput clinical laboratory testing. It is capable of performing a wide range of clinical chemistry, immunoassay, and specialized diagnostic tests. The system features advanced automation and workflow optimization to deliver efficient and reliable results.
Automatically generated - may contain errors
4 protocols using architect i4000sr system
1
Serum LH, Testosterone, and Estradiol Assay
2
Comprehensive Biomarker Assessment for HBV
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Blood samples were collected at every visit time. Routine blood testing was performed on Siemens ADVIA 2120 Hematology Analyzer. Routine biochemical tests including alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), etc. were measured by automated biochemical technique (Siemens Healthcare Diagnostics, USA). Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech, China) on ABI7500 Real‐Time PCR System (Life Technologies, USA), which has a detection limit of 500 IU/mL. The levels of HBsAg, HBsAb, HBeAg, HBeAb and HBcAb were measured using a commercial chemiluminescent microparticle immunoassay kit with the Architect i4000SR System (Abbott Laboratories, USA).
3
Quantitative Tumor Marker Detection
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A total of 3 mL of venous blood was collected to obtain serum through centrifugation at 404 × g for 10 min, with the purpose of quantitatively detecting multiple tumor markers. According to the manufacturer's instructions, the fluorescence signal was captured using a chemiluminescence immunoassay kit (Abbott, ARCHITECT i4000SR System). The critical values for the markers were as follows: CEA > 5.0 ng/mL, SCCA > 1.5 ng/mL, and CYFRA21-1 > 3.3 ng/mL. Serum samples were analyzed immediately.
4
Routine Biochemical and Serological Analysis of Hepatitis B
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Routine biochemical tests including total protein (TP), albumin (Alb), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil), direct bilirubin (DB), and indirect bilirubin (IDB) were detected by automated biochemical technique (Siemens Healthcare Diagnostics, USA). Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech, China) on ABI 7500 Real‐Time PCR System (Life Technologies, USA), which has a detection limit of 20 IU/mL. HBsAg, HBsAb, HBeAg, HBeAb, and HBcAb were measured using a commercial chemiluminescent microparticle immunoassay kit with the Architect i4000SR System (Abbott Laboratories, USA).
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