Healthy-hMSCs or CKD-hMSCs and THP-1 cells were co-cultured in Millicell Cell Culture Plates (Millipore, Billerica, MA, USA), in which culture media are in indirect contact with cells. TH-1 cells were seeded in the lower compartments, and then exposed to P-cresol for 24 h, and then healthy-hMSCs, CKD-hMSCs, TUDCA-treated CKD-hMSCs (TUDCA+CKD-hMSC), TUDCA-pretreated CKD-hMSCs treated with si-PRNP (si-PRNP+CKD-hMSC), or TUDCA-pretreated CKD-hMSCs treated with si-Scr (si-Scr+CKD-hMSC) were seeded onto the transwell membrane inserts for incubation for 48 h. They were incubated in a humidified atmosphere containing 5% of CO2 at 37 °C. TH-1 cell proliferation, expression of PrPC, MitoSOX, and complex Ⅰ & Ⅳ activities were assessed as described above for the proliferation assay, ELISAs, and FACS analysis.
Millicell cell culture plates
Manufactured by Merck Group
Sourced in United States
Millicell Cell Culture Plates are a line of laboratory equipment designed for cell culture applications. They provide a controlled and standardized environment for the growth and maintenance of cells. The plates are made of high-quality materials and are available in various sizes and well configurations to accommodate different experimental needs.
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Lab products found in correlation
3 protocols using millicell cell culture plates
1
Co-culture of hMSCs and THP-1 Cells
2
Co-culture of SH-SY5Y and hMSCs
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SH-SY5Y cells and hMSCs were co-cultured in Millicell Cell Culture Plates (Millipore, Billerica, MA, USA) in a humidified 5% CO2 incubator at 37 °C. SH-SY5Y cells were seeded in the lower compartments, followed by exposure to P-cresol for 24 h. MSCs were then seeded onto the transwell membrane inserts and the plates were incubated for a further 24 h, following which the SH-SY5Y cells were assessed by several assays.
3
Coculture Analysis of CD34+ Cells
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Coculture analysis was performed in 12-well Millicell Cell Culture Plates (0.4 µm pore size; Millipore, Billerica, MA, USA) using Stem Span serum-free medium (StemCell Technologies), supplemented with 50 ng/mL VEGF, 20 ng/mL IL-6 (R&D Systems), 100 ng/mL SCF (Kirin), 20 ng/mL thrombopoietin (TPO; Wako), 100 ng/mL Flt-3 ligand (Wako), and 1% penicillin-streptomycin (WELGENE). Human CD34+ cells isolated from HUCB were seeded in the lower compartment of the transwell, and the transwell membrane inserts were seeded with human CD34− cells isolated from HUCB or left unseeded. To measure the EPC-CFU potential of CD34+ cells after coculture, the transwell inserts were removed after 7 d. The expansion of CD34+ cell was determined by cell counting.
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