Recombinant rat IL-1β was obtained from Peprotech (Rocky Hill, NJ, USA). EPZ005687 was obtained from MedChemExpress (1396772-26-1). Antibodies against EZH2 and Sox-9 were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against H3K27me3 and normal rabbit IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against β-actin, aggrecan, Collagen II, ADAMTS5 and MMP13 were purchased from Abcam (Cambridge, MA, USA).
Epz005687
Manufactured by MedChemExpress
EPZ005687 is a small molecule compound that inhibits the enzymatic activity of EZH2, a histone methyltransferase that plays a role in epigenetic regulation. This compound can be used as a research tool to study the biological functions of EZH2 and its involvement in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Mmp13, by Abcam (1 mentions)
Recombinant rat il 1β, by Thermo Fisher Scientific (1 mentions)
Adamts5, by Abcam (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti ezh2, by Abcam (1 mentions)
Irdye 680 goat anti mouse secondary antibody, by LI COR (1 mentions)
Anti sfrp1, by Abcam (1 mentions)
Recombinant human and mouse il 1β, by R&D Systems (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Anti h3k27me3, by Merck Group (1 mentions)
2 protocols using epz005687
1
Recombinant Rat IL-1β and EZH2 Pathway
2
Investigating EZH2 and Wnt Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco’s Modified Eagle’s Medium: Nutrient Mixture F-12 (DMEM/F-12) Media was obtained from Hyclone (Utah, USA). Recombinant human and mouse IL-1β were obtained from R&D Systems (Minneapolis, MN, USA). Penicillin, streptomycin and fetal bovine serum (FBS) were obtained from Gibco BRL (Grand Island, NY, USA). EPZ005687 was obtained from MedChemExpress _(1396772-26-1). The following antibodies were used in this study: anti-EZH2 from Abcam (Cell Signaling Technology); anti-SOX9 and anti-β-catenin from Cell Signaling Technology (Danvers, MA, USA); anti-H3K27me3 (Millipore, CA, US); normal rabbit IgG (Santa Cruz, Heidelberg, Germany); anti-SFRP-1 (Abcam, Cambridge, UK); Alexa-Fluor-488- and Alexa-Fluor-545- conjugated secondary antibodies from Molecular Probes (Eugene, OR, USA); and goat anti-rabbit IRDye 800CW and goat anti-mouse IRDye 680 secondary antibodies from LI-COR Biosciences (Lincoln, NE, USA).
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