Clarus 500 sq 8 s

Cellular fatty acid methyl ester (FAME) analysis was performed by GC/MS. Two samples were prepared with approximately 2 mg of bacterial biomass each, harvested from five culture plates. Fatty acid methyl esters were prepared as described by Sasser (2006). GC/MS analyses were carried out as described previously (Dione et al., 2016). In brief, fatty acid methyl esters were separated using an Elite 5‐MS column and monitored by mass spectrometry (MS) (Clarus 500‐SQ 8 S, PerkinElmer, Courtaboeuf, France). A spectral database search was performed using MS Search 2.0 operated with the Standard Reference Database 1A (NIST, Gaithersburg, MD, USA) and the FAMEs mass spectral database (Wiley, Chichester, UK).

GC/MS was used for cellular fatty acid methyl ester (FAME) analysis after preparing two cell samples containing around 15 mg of bacterial biomass per tube and analysis was performed as previously described (Dione et al., 2016). Moreover, Elite 5‐MS column was used for FAME separation and checked by mass spectrometry (Clarus 500‐SQ 8 S, Perkin Elmer, Courtaboeuf, France). Then, the FAME mass spectral database (Wiley, Chichester, UK) and MS Search 2.0 operated with the Standard Reference Database 1A (NIST, Gaithersburg, USA) were used for FAME identification.

Biochemical tests were performed using API ZYM, API 20A, and API 50CH strips (bioMérieux) according to the manufacturer's instructions. The strips were incubated for 4, 24, and 48 hr respectively.
Cellular fatty acid methyl ester (FAME) analysis was performed using Gas Chromatography/Mass Spectrometry (GC/MS). Strain Marseille‐P2341^T was grown on Columbia agar enriched with 5% sheep's blood (bioMérieux). Two samples were then prepared with approximately 50 mg of bacterial biomass per tube harvested from several culture plates. Fatty acid methyl esters were prepared as described by Sasser (Sasser, 2006). GC/MS analyses were carried out as previously described (Dione et al., 2016). In brief, fatty acid methyl esters were separated using an Elite 5‐MS column and monitored by mass spectrometry (Clarus 500—SQ 8 S, Perkin Elmer, Courtaboeuf, France). A spectral database search was performed using MS Search 2.0 operated with the Standard Reference Database 1A (NIST, Gaithersburg, USA) and the FAMEs mass spectral database (Wiley, Chichester, UK).
Antibiotic susceptibility was tested using the disc diffusion method (Le Page et al., 2015). The results were read using Scan 1200 (Interscience, Saint‐Nom‐la‐Bretèche, France).

Biochemical characteristics of the strains were investigated using API ZYM, 20A and 50CH strips (BioMérieux) according to the manufacturer's instructions. A 20‐min‐thermic shock of fresh colonies at 80°C was done in order to test sporulation. Catalase (BioMerieux) activity was determined in 3% hydrogen peroxide solution and oxidase activity was assessed using an oxidase reagent (Becton‐Dickinson).
Cellular fatty acid methyl ester (FAME) analysis was performed by gas chromatography/mass spectrometry (GC/MS). Two samples were prepared with approximately 17 mg of bacterial biomass per tube for strain Marseille‐P2849^T and 5 mg per tube for strain Marseille‐P3277^T. Briefly, fatty acid methyl esters were separated using an Elite 5‐MS column and monitored by mass spectrometry (Clarus 500—SQ 8 S, Perkin Elmer, Courtaboeuf, France) as previously described (Dione et al., 2016). Spectral database search was performed using MS Search 2.0 operated with the Standard Reference Database 1A (NIST, Gaithersburg, USA) and the FAMEs mass spectral database (Wiley, Chichester, UK).
Antibiotic susceptibility was tested using the E test gradient strip method (BioMerieux) to determine the minimal inhibitory concentration (MIC) of each tested antibiotic on blood Colombia agar media (BioMerieux, France).

Cellular fatty acid methyl ester (FAME) analysis was performed by GC/MS for both Marseille-P9602 and LMG 13028^T strain. Fatty acid methyl esters were prepared as described by Sasser⁵² and GC/MS analysis was realized as previously described^{53 (link)}. Briefly, Marseille-P9602 and LMG 13028^T strains were inoculated in 5% sheep blood-enriched Columbia agar and incubated at 28 °C. Fatty acid methyl esters were separated using an Elite 5-MS column and monitored by mass spectrometry (Clarus 500—SQ 8 S, Perkin Elmer, Courtaboeuf, France). Spectral database search was performed using MS Search 2.0 operated with the Standard Reference Database 1A (NIST, Gaithersburg, USA) and the FAMEs mass spectral database (Wiley, Chichester, UK).

Cellular fatty acid methyl ester (FAME) analysis was performed by GC/MS, as previously described [59 (link),60 (link)]. Fatty acid methyl esters were prepared as described by M. Sasser [61 ]. Briefly, fatty acid methyl esters were analyzed by gas chromatography/mass spectrometry (GC/MS). Compounds were separated using an Elite 5-MS column and monitored by mass spectrometry (Clarus 500—SQ 8 S, Perkin Elmer, Courtaboeuf, France). Spectral database search was performed using MS Search 2.0 operated with the Standard Reference Database 1A (NIST, Gaithersburg, MD, USA) and the FAMEs mass spectral database (Wiley, Chichester, UK).