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Retroviral pmx vector

Manufactured by Cell Biolabs
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The Retroviral pMX vector is a plasmid-based expression system designed for the production of recombinant retroviruses. The vector contains the necessary genetic elements for viral packaging and gene delivery, allowing for the efficient transduction of target cells with the gene of interest.

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Lab products found in correlation

Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PMXs-IRES-Blasticidin Retroviral vector PMXs-miR-GFP/puro retroviral expression vector PMXs-IRES-GFP retroviral vector N-MYC-tagged pMXs-Puro Retroviral Vector PMXs-puro retroviral vector N-MYC-tagged pMX-Puro Retroviral Vector

2 protocols using retroviral pmx vector

1

Transient Expression of mDia2 and ATP Biosensors in Human Fibroblasts

Cited 2 times
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Wild-type or mutant Flag-tagged mDia2 cDNAs (17, 20 (link)) were subcloned into the retroviral pMX vector (Cell Biolabs). pGEM-MIRO1 and cyto-Ruby3-iATPSnFR1.0 (ATP biosensors; ref. 21 (link)) plasmids were purchased from Sino Biological Inc. (#HG15898-G) or Addgene (#102551), respectively. Plasmid vectors and Lipofectamine 2000 (#11668030, Invitrogen) were incubated with Opti-MEM I Reduced Serum Medium (#31985062, Thermo Fisher Scientific) at room temperature for 20 minutes. The mixture was then added to primary human fibroblasts. After 6 hours of incubation, the medium was replaced by a normal culture medium containing 10% FBS. Cells were allowed to recover for 24–48 hours prior to analysis.
Cangkrama M., Liu H., Whipman J., Zubair M., Matsushita M., Di Filippo M., Kopf M., Innocenti M, & Werner S. (2022). A Protumorigenic mDia2–MIRO1 Axis Controls Mitochondrial Positioning and Function in Cancer-Associated Fibroblasts. Cancer Research, 82(20), 3701-3717.
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2

Overexpression of Flag-mDia2 in Fibroblasts

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Wild‐type Flag‐tagged mDia2 cDNA (Isogai et al, 2015a, 2016) was subcloned into the retroviral pMX vector (Cell Biolabs). One microgram pMX Flag‐mDia2 or empty vector and Lipofectamine™ 2000 (#11668030, Invitrogen) were incubated with Opti‐MEM™ I Reduced Serum Medium (#31985062, Thermo Fisher Scientific) at room temperature for 20 min. The mixture was then added to primary human fibroblasts. After 6‐h incubation, medium was replaced by normal culture medium containing 10% FBS. Cells were allowed to recover for 48 h prior to analysis.
Cangkrama M., Wietecha M., Mathis N., Okumura R., Ferrarese L., Al‐Nuaimi D., Antsiferova M., Dummer R., Innocenti M, & Werner S. (2020). A paracrine activin A–mDia2 axis promotes squamous carcinogenesis via fibroblast reprogramming. EMBO Molecular Medicine, 12(4), e11466.
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