Anti igd 11 26c 2a

Manufactured by BioLegend

Anti-IgD (11-26c.2a) is a monoclonal antibody produced by BioLegend that recognizes the IgD molecule. This antibody can be used for the detection and quantification of IgD in various immunological applications.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (1 mentions) Anti vcam1 429, by BD (1 mentions) Anti madcam1 meca367, by BioXCell (1 mentions) Anti cd35 8c12, by BD (1 mentions) Rat igg2a isotype control 2a3, by BioXCell (1 mentions) Fluorescent mounting medium, by Agilent Technologies (1 mentions) Avidin biotin blocking kit, by Vector Laboratories (1 mentions) Anti il 17a, by BioLegend (1 mentions) Anti cd3 17a2, by BioLegend (1 mentions) Eclipse 80i microscope, by Nikon (1 mentions) Anti cd19 6d5, by BioLegend (1 mentions) Anti cd138 281 2, by BioLegend (1 mentions) Anti f4 80 bm8, by BioLegend (1 mentions) Anti b220 ra3 6b2, by BD (1 mentions) Anti igm rmm1, by BioLegend (1 mentions) Zombie aqua, by BioLegend (1 mentions) Fitc dextran, by Merck Group (1 mentions) Np2 bsa, by Biosearch Technologies (1 mentions) Anti gfp antibody, by Abcam (1 mentions) Anti fas jo2, by BD (1 mentions)

Spelling variants of this product

IgD (11-26c.2a; (10 citations) Anti-IgD (5 citations)

7 protocols using anti igd 11 26c 2a

Multiparameter Flow Cytometry Analysis of Murine Immune Cells

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Mouse splenocytes or peripheral blood were hemolyzed with ACK buffer, stained with fluorescently conjugated antibodies analyzed on a BD LSR II flow cytometer. The following antibody clones were used in this study: anti-CD122 (TMB1), anti-CD44 (IM7), anti-CD3 (17A2), anti-CD8 (53-6.7) anti-CD19 (6D5) anti-B220 (RA3-6B2), anti-CD21(7E9), anti-IgM (RMM-1), anti-CD1d (1B1), and anti-IgD (11-26c.2a) from Biolegend.

Mahajan V.S., Demissie E., Mattoo H., Viswanadham V., Varki A., Morris R, & Pillai S. (2016). Striking immune phenotypes in diverse gene-targeted mice are driven by a copy number variant originating from a commercially available C57BL/6 strain. Cell reports, 15(9), 1901-1909.

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Immunohistochemical Staining of Cryosections

Cited 1 time

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Cryosections 7um in thickness were cut and fixed in cold acetone.
Sections were stained with anit-ICAM1 (3E2; BD Biosciences); anti-VCAM1 (429; BD
Biosciences); anti-MADCAM1(MECA367; Bio X Cell); anti-IgD (11-26c.2a;
Biolegend); anti-Vitronectin (347317; R&D); rat IgG2a isotype control
(2A3; Bio X Cell) or anti-CD35 (8C12; BD Biosciences) using described protocols
(36 (link)).

Wang X., Rodda L., Bannard O, & Cyster J.G. (2014). Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4601-4609.

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Immunofluorescence Analysis of Murine Spleen

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Spleens from mice were collected, embedded in Tissue-Tek (4583 BioLab) and snap-frozen on dry ice. Frozen sections (thickness, 5 μm) were fixed in acetone. Before staining, sections were blocked with 5% rat serum for 30 min. Possible biotin- or streptavidin-binding sites were blocked using the Avidin/Biotin Blocking Kit from Vector. Afterward, the sections were stained with various antibodies. The following fluorochrome- or biotin-conjugated anti-mouse antibodies and reagents were used: anti-CD3 (17A2; 1:50), anti-IL-17A (TC11-18H10.1; 1:20), anti-RORγt (B2D; 1:20), anti-IgD (11-26c-2a; 1:50), rat IgG1 isotype-matched control antibody (RTK2071) and rat IgG2a isotype (RTK2758) (all from BioLegend); and anti-CD21/35(8D9; 1:50) (from eBioscience). Sections were mounted in fluorescent mounting medium (Dako) and analyzed with an Eclipse-80i microscope (Nikon). Pictures are presented with pseudocolors (as indicated in figures) by NIS elements software BR3.0 (Nikon).

Pfeifle R., Rothe T., Ipseiz N., Scherer H.U., Culemann S., Harre U., Ackermann J.A., Seefried M., Kleyer A., Uderhardt S., Haugg B., Hueber A.J., Daum P., Heidkamp G.F., Ge C., Böhm S., Lux A., Schuh W., Magorivska I., Nandakumar K.S., Lönnblom E., Becker C., Dudziak D., Wuhrer M., Rombouts Y., Koeleman C.A., Toes R., Winkler T.H., Holmdahl R., Herrmann M., Blüml S., Nimmerjahn F., Schett G, & Krönke G. (2016). Regulation of autoantibody activity by the IL-23–TH17 axis determines the onset of autoimmune disease. Nature immunology, 18(1), 104-113.

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Multiparameter Immune Cell Profiling

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Spleens and bone marrow (BM) from naïve mice were harvested from age- and sex-matched cohorts, and red blood cells were lysed by ammonium chloride lysis. Splenocytes were incubated for 30 minutes on ice with the following antibodies and dyes: viability (Zombie Aqua, Biolegend), anti-B220 (RA3–6B2, BD Biosciences), anti-CD19 (6D5, Biolegend), anti-CD23 (B3B4, Biolegend), anti-CD93 (AA4.1, Biolegend), anti-Ly77 (GL7, Biolegend), anti-IgD (11-26C.2a, Biolegend), anti-IgM (RMM1, Biolegend). and anti-F4/80 (BM8, Biolegend). BM was incubated for 30 minutes on ice with the following antibodies and dyes: anti-B220 (RA3–6B2, BD Biosciences), anti-CD138 (281-2, Biolegend), anti-VpreB (R3, Biolegend), and anti-CD24 (M1/69, Biolegend). FCRL1 expression in different cell types in the bone marrow and spleen was tracked with anti-CD307a (REA566, Miltenyi).

DeLuca J.M., Murphy M.K., Wang X, & Wilson T.J. (2021). FCRL1 regulates BCR-induced ERK activation through GRB2. Journal of immunology (Baltimore, Md. : 1950), 207(11), 2688-2698.

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Murine B Cell Phenotyping and Detection

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Fluorophore-labeled anti-B220 (RA3-6B2) and anti-FAS (Jo2) antibodies were from BD; anti-CD138 (281-2), anti-CD21/35 (CR2/CR1), and anti-IgD (11-26c2a) antibodies were from BioLegend; anti-GL7 (GL7), anti-CD38 (90), and anti-rabbit IgG antibodies were from Invitrogen; rabbit anti-cCasp3 and the matching isotype control antibodies were from Cell Signaling; and anti-GFP antibody was from Abcam. The fixable viability dye, zombie yellow, was from BioLegend. NP₁₈-OVA, NP₃₀-Ficoll, NP₂-BSA, NP-PE, and 4-hydroxy-3-iodo-5-nitrophenylacetyl (NIP)-BSA-biotin were from Biosearch Technologies. NP-APC conjugates were made by allowing NP-Osu (Biosearch Technologies) and APC (BioLegend) to react for 4 h at room temperature in a buffer containing 0.1 M NaHCO₃ and 0.15 M NaCl₂ (pH 8), at a molar ratio of 20:1. Dextran (200 kD, 31398) and Dextran-FITC (2,000 kD, FD2000S) were from Sigma-Aldrich.

Liu X., Zhao Y, & Qi H. (2022). T-independent antigen induces humoral memory through germinal centers. The Journal of Experimental Medicine, 219(3), e20210527.

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Immunofluorescence Staining of Human Tonsils and Mouse Spleen

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For human tonsils, frozen sections of tonsils, 6 μm in thickness, were fixed with cold acetone and stained with anti-CD3 (UCHT1l, BD Biosciences) and anti-Tox2 (LS-C29895, LSBio) followed by Alexa Fluor 568–conjugated anti-mouse IgG1 (A-21124, Molecular Probes) and Alexa Fluor 488–conjugated anti-rabbit (A-11070, Molecular Probes). Last, sections were counterstained for 2 min with 3 μM DAPI (4′,6-diamidino-2-phenylindole). For mice spleen, frozen sections, 6 μm in thickness, were fixed with True-Nuclear buffer (BioLegend) and stained with anti-CD4 (GK 1.5) and anti-Bcl6 (K112-91, BD Biosciences) at 4°C overnight followed by 20 min at room temperature for anti-IgD (11-26c.2a, BioLegend) in Perm/Wash buffer (BioLegend). Sections were mounted with ProLong Glass Antifade Mountant with NucBlue (Invitrogen) for DAPI counterstaining and sealing. Slide images were observed under a Leica SP5 confocal microscope with a PlanApo 20× objective (numerical aperture, 0.7) and a PlanApo 40× objective (numerical aperture, 1.25).

Horiuchi S., Wu H., Liu W.C., Schmitt N., Provot J., Liu Y., Bentebibel S.E., Albrecht R.A., Schotsaert M., Forst C.V., Zhang B, & Ueno H. (2021). Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells. Science Advances, 7(41), eabj1249.

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Immunohistochemical Staining of Mouse Spleen

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Spleens of recipient mice were frozen in OCT compound (Tissue-Tek; Sakura). Sections were cut into 7-μm slices with a microtome at −20 °C and fixed in cold acetone and then blocked with blocking buffer (Protein Block Serum-Free, Dako). Cryosections were incubated with primary antibodies, followed by staining with DyLight 488-conjugated or DyLight 594-conjugated secondary antibodies (The Jackson Laboratories).
The primary antibodies were as follows: anti-IgD (11–26c.2a, BioLegend), anti-MAdCAM-1 (MECA-367, BioLegend), anti-CD4 (GK1.5, eBioscience), and anti-PD-1 (RMP1-30, BioLegend). Rhodamine-conjugated peanut agglutinin (PNA, Vector laboratories) was used for staining of GCs. For the staining of Thy1.1, sections were incubated with biotinylated anti-CD90.1 (OX-7) mAb (AbD Serotec) followed by Streptavidin Alexa 488 (Life Technologies). Images were captured using an Olympus BX 51 fluorescent microscope equipped with an Olympus DP 71 digital camera (Olympus, Tokyo, Japan).

Eri T., Kawahata K., Kanzaki T., Imamura M., Michishita K., Akahira L., Bannai E., Yoshikawa N., Kimura Y., Satoh T., Uematsu S., Tanaka H, & Yamamoto K. (2017). Intestinal microbiota link lymphopenia to murine autoimmunity via PD-1+CXCR5−/dim B-helper T cell induction. Scientific Reports, 7, 46037.

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