Anti mouse cd86 gl1

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-mouse CD86 (GL1) is a monoclonal antibody that recognizes the mouse CD86 antigen. CD86 is a costimulatory molecule expressed on antigen-presenting cells and plays a key role in T cell activation.

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Lab products found in correlation

Anti mouse cd19 1d3, by BD (1 mentions) Alexa 647 conjugated streptavidin, by Thermo Fisher Scientific (1 mentions) Facs canto 1 flow cytometer, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Diva software, by BD (1 mentions) L tryptophan, by Merck Group (1 mentions) Anti β actin, by Abmart (1 mentions) Ifn γ and il 4, by Thermo Fisher Scientific (1 mentions) L alanine, by Merck Group (1 mentions) Polybrene, by Merck Group (1 mentions)

Close spelling variants of this product

CD86 (GL1, (9 citations) Anti-CD86 (32 citations)

2 protocols using anti mouse cd86 gl1

Mouse Spleen B Cell Profiling

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Mouse spleen B cells overnight cultured in medium or in presence of LPS (100 ng-1 μg/ml, Sigma-Aldrich) were incubated with anti-mouse CD86 (GL1, eBioscience) and anti-mouse CD19 (1D3, BD Bioscience) antibodies and analyzed by FACS canto II (Becton Dickinson, Franklin Lakes, NJ, USA).
IL-1R8 cell surface staining on human cells was performed with biotinylated goat anti-human IL-1R8/SIGIRR (R&D Systems), followed by Alexa-647 conjugated streptavidin (Molecular Probes, Invitrogen), and analyzed with FACS Canto I flow cytometer (BD Bioscience). Diva software (BD Pharmingen) and Flow-jo (Tree Star) were used for data acquisition and analysis, respectively.

Riva F., Ponzoni M., Supino D., Bertilaccio M.T., Polentarutti N., Massara M., Pasqualini F., Carriero R., Innocenzi A., Anselmo A., Veliz-Rodriguez T., Simonetti G., Anders H.J., Caligaris-Cappio F., Mantovani A., Muzio M, & Garlanda C. (2019). IL-1R8 Deficiency Drives Autoimmunity-Associated Lymphoma Development. Cancer immunology research, 7(6), 874-885.

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Evaluating Immunomodulatory Mechanisms

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L-tryptophan, L-alanine, LPS (Escherichia coli 055:B5), 1-methyl-L-tryptophan (L-1-MT), 1-methyl-D-tryptophan (D-1-MT), diphenylene iodonium (DPI), HPLC-grade methanol (MeOH), and polybrene were obtained from Sigma–Aldrich (St. Louis, MO). IFN-γ and IL-4 were from PeproTech. All primers were synthesized by Sangon Biotech. Anti-IL-10Rα blocking antibody was from R&D Systems (Minneapolis, MN, USA). Mouse anti-IL4I1 monoclonal antibody was generated by AbMart (www.ab-mart.com.cn). Anti-β-actin and Glyceraldehyde 3-phosphate dehydrogenase (GADPH) monoclonal antibodies were also from AbMart. Rabbit monoclonal anti-Myc epitope-tagged antibody, and phospho-STAT6 (Tyr641), phospho-STAT3 (Tyr705), total STAT-3, and total STAT-6 monoclonal antibodies were from Cell Signaling Technologies (Danvers, MA, USA). Anti-mouse CD11b (M1/70), anti-mouse Ly-6G (1A8), anti-mouse F4/80 (BM8), anti-mouse MHC Class II (M5/114.15.2), anti-mouse CD80 (16-10A1), and anti-mouse CD86 (GL1) antibodies were from eBioscience. Ovalbumin (OVA)_323–339 peptide was from Chinese Peptide Co.

Yue Y., Huang W., Liang J., Guo J., Ji J., Yao Y., Zheng M., Cai Z., Lu L, & Wang J. (2015). IL4I1 Is a Novel Regulator of M2 Macrophage Polarization That Can Inhibit T Cell Activation via L-Tryptophan and Arginine Depletion and IL-10 Production. PLoS ONE, 10(11), e0142979.

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