Purified anti human il 1β 511601

Manufactured by BioLegend

Purified anti-human IL-1β (511601) is an antibody product that binds to the human interleukin-1 beta (IL-1β) protein. IL-1β is a proinflammatory cytokine that plays a key role in the immune response and inflammatory processes. This product can be used for research purposes, such as in immunoassays or other applications that require a specific antibody targeting human IL-1β.

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Lab products found in correlation

Aminoguanidine hydrochloride 396494, by Merck Group (2 mentions) Mouse monoclonal anti β actin antibody a1978, by Merck Group (1 mentions) Anti β actin a1978 antibody, by Merck Group (1 mentions)

Close spelling variants of this product

Human IL-1β Anti-IL-1β IL-1β

2 protocols using purified anti human il 1β 511601

Optimizing Western Blot Conditions

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Rabbit anti-IDO1 polyclonal antibody (13268-1-AP) was obtained from Proteintech. Mouse anti-iNOS monoclonal antibody (NOS2; sc-7271) was obtained from Santa Cruz Biotechnology. Mouse anti-β-Actin monoclonal antibody (A1978) was obtained from Sigma. Recombinant human IFN-γ and IL-1β were obtained from Peprotech. Aminoguanidine hydrochloride (396494) was obtained from Sigma. Purified anti-human IL-1β (511601) and biotin anti-human IL-1β antibodies (511703) were obtained from BioLegend.

Bando H., Lee Y., Sakaguchi N., Pradipta A., Sakamoto R., Tanaka S., Ma J.S., Sasai M, & Yamamoto M. (2019). Toxoplasma Effector GRA15-Dependent Suppression of IFN-γ-Induced Antiparasitic Response in Human Neurons. Frontiers in Cellular and Infection Microbiology, 9, 140.

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Immunoblotting Assay for Inflammatory Mediators

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Antibodies against IDO1 (13268-1-AP), NLRP1 (12256-1-AP), NLRP3 (19771-1-AP), and caspase-1 (22915-1-AP) were obtained from Proteintech. Antibodies against Irgm1 (LRG47; sc-11075), IL-1R1 (H-8; sc-393998), and iNOS (NOS2; sc-7271) were obtained from Santa Cruz Biotechnology. Anti-IRGM antibody (ABC338) was obtained from Merck Millipore. Anti-β-actin antibody (A1978) was obtained from Sigma. Anti-MyD88 antibody (2127) and anti-ATG16L1 antibody (PM040) were obtained from ProSci and MBL, respectively. Antibodies against GAP45 were described previously (59 (link)). Recombinant human and mouse IFN-γ, IL-1α, and IL-1β were obtained from Peprotech. Aminoguanidine hydrochloride (396494) was obtained from Sigma. Purified anti-human IL-1β (511601) and biotin anti-human IL-1β (511703) were obtained from BioLegend.

Bando H., Lee Y., Sakaguchi N., Pradipta A., Ma J.S., Tanaka S., Cai Y., Liu J., Shen J., Nishikawa Y., Sasai M, & Yamamoto M. (2018). Inducible Nitric Oxide Synthase Is a Key Host Factor for Toxoplasma GRA15-Dependent Disruption of the Gamma Interferon-Induced Antiparasitic Human Response. mBio, 9(5), e01738-18.

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