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3 protocols using 6 hydroxy 2 5 7 8 tetramethyl chroman 2 carboxylic acid trolox

1

Antioxidant Evaluation of Sea Buckthorn and Microbial Cultures

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Folin–Ciocalteu reagent and 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox) were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). 2,2-diphenyl-1-picrylhydrazyl (DPPH) and gallic acid were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). 2,2′–azino-bis-(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ) were purchased from Aladdin Industrial Corporation (Shanghai, China). Ethanol absolute, methanol anhydrous, sodium carbonate, potassium persulfate, glacial acetic acid, sodium acetate, acetic acid, iron trichloride, and hydrochloric acid (HCL) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Sea buckthorn (named “shengqiuhong”, Fructus Hippophae, produced in Tacheng, Xinjiang, China) was provided by Gansu Aikang Sea buckthorn Products Co.,Ltd. (Gansu, China). Lactobacillus paracasei was provided by Xi’an Jushengyuan Biotechnology Co., Ltd.(Xi’an, China). Saccharomyces cerevisiae was provided by Angelyeast Inc.(Hubei, China). Sucrose was from the local market.
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2

Antioxidant Activity Assays of Whey Protein Isolate

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WPI (Item No. 9410) was obtained from Hilmar ingredients Co. Ltd. (CA, U.S.A.). Ethanol (purity >99.9%) was purchased from Tianli Chemical Reagent Co. Ltd. (Tianjin, China). 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were purchased from Yuanye Bio-Technology Co. Ltd. (Shanghai, China). Pyridine was obtained from Aladdin Reagent Co. Ltd. (Shanghai, China). Fluorescein sodium and 5′,5-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich Co. Ltd. (St. Louis, MO, USA). All other chemicals and reagents used in this study were of analytical grade.
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3

Antioxidant Capacity Assay Protocol

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2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH), (±)‐6‐hydroxy‐2,5,7,8‐tetramethyl‐ chroman‐2‐carboxylic acid (Trolox), 2,4,6‐tri‐2‐pyridyl‐1,3,5‐triazine (TPTZ), and foline‐phenol were purchased from Shanghai Yuanye Bio‐Technology Co. Anhydrous ferric chloride and anhydrous sodium acetate were purchased from Sinopharma Chemical Reagent Co. Methanol of HPLC grade was purchased from Beijing Chemicals Co. Other chemicals were obtained from Beijing Solarbio Technology Co.
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