Ultrafast scanner
The UltraFast Scanner is a high-performance laboratory equipment designed for rapid data acquisition. It features advanced scanning technology capable of capturing images and data at exceptional speeds. The core function of the UltraFast Scanner is to provide efficient and accurate data collection for various scientific and research applications.
Lab products found in correlation
22 protocols using ultrafast scanner
Immunohistochemical Detection of Amyloid-β
Immunostaining and Quantification in Rodents
Immunohistochemical Analysis of CCL21 and CCR7
Automating PD-L1 Assessment in Cancer
Immunohistochemical Analysis of Mantle Cell Lymphoma
The list of antibodies used in the immunohistochemical analysis.
Antibody | Clone | Company |
---|---|---|
BCL2 | 124 | DAKO |
BCL6 | PG-B6p | DAKO |
BSAP (PAX-5) | DAK-Pax5 | DAKO |
CD3 | Polyclonal rabbit | DAKO |
CD5 | 4C7 | DAKO |
CD10 | 56C6 | DAKO |
CD20 | L26 | DAKO |
CD38 | SP149 | Cell Marque |
Cyclin D1 | EP12 | DAKO |
MYC | Y69 | VENTANA |
Ki-67 | MIB-1 | DAKO |
MUM-1 | MUM1p | DAKO |
SOX-11 | MRQ-58 | Cell Marque |
TdT | EP266 | DAKO |
The H&E slides were scanned using UltraFast Scanner (Philips IntelliSite Solution, USA) with DigiPath™ Professional Production Software (Xerox, Norwalk, CT, USA) and representative areas of each case were selected and microtomed into 8 µm thick sections and mounted onto 1-mm-thick calcium fluoride (CaF2) windows (Crystran, UK).
Digitization Errors in Whole Slide Imaging
Quantification of Lung Metastatic Burden
Protein Expression and Immunostaining
Paraffin-embedded tumor sections were deparaffinized by successive incubation in xylene, 95% ethanol, 90% ethanol, and 70% ethanol, followed by PBS. Epitopes were unmasked by steaming in DAKO antigen retrieval solution for 45 min and then rinsed twice in PBS. The sections were blocked using DAKO blocking solution, and then immunostained overnight at 4 °C using primary antibodies (MYC, 1:150, Epitomics). Sections were then washed with PBS and incubated with biotinylated anti-rabbit IgG (1:300, Vector Labs) for 30 min at room temperature, then with the ABC kit (Vector Labs) for 30 min at room temperature. Sections were developed using 3,3′-diaminobenzidine (DAB, Vector Labs), counterstained with hematoxylin, and mounted with Permount. Images were obtained on a Philips Ultrafast Scanner.
HER2 Immunohistochemistry and Digital Image Analysis
HER2 DIA scores were categorized into four categories with the following cutoff values: (1) 0: connectivity = 0; (2) 1+: 0
Breast Cancer Immunohistochemistry Quantification
Annotations were marked at region level and cell level to develop ML models for region segmentation and cell detection and classification. Region annotations included tumor, ductal carcinoma in situ (DCIS), and normal regions while cell annotation included Ki67‐positive tumor, Ki67‐negative tumor, positive nontumor, and negative nontumor cells. In total, we collected annotations for around 600 mm2 of tumor area, 140 mm2 of normal area, 380 mm2 of DCIS area, and 9,433, 26,308, 2,424, 19,223 of the four cell types, respectively.
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