Magnetom skyra 3.0t

Patients underwent a secondary MRI and CT examination 7 d after admission and 7 d before discharge. CT examination was performed with a Siemens raw 16-slice spiral CT system, with an axial scanning routine, a controlled layer thickness of 10 mm, and a remaining layer pitch of 10 mm. MRI examination was performed with a Siemens MAGNETOM Skyra3.0T superconducting MRI, and the data parameters used have been reported previously: Rectangular array parameter of 256 × 384, auxiliary sagittal and coronal T1WI, FLAIR (TE 72 ms, TR 6000 ms), TSE T2WI (TR 4600 ms, TE 103 ms), and DWI (TR 6202 ms, TE 92 ms), seT1WI (TR 500 ms, TE 12 ms), and the brain was scanned 2 to 3 times at 1-mm intervals. After completing the routine examinations, all patients underwent enhanced scans, and after obtaining the image data, these were handed over to the senior attending doctor of our hospital for diagnosis.

The ultrasonographic examination was performed with Voluson E8 and Logiq E9 color ultrasound instruments (GE Healthcare, Chicago, IL, USA) using a convex array probe with a frequency of 2.5 to 5.0 MHz and a linear array probe with a frequency of 6 to 15 MHz. Contrast-enhanced ultrasonography was performed with intravenous injection of SonoVue (Bracco, Milan, Italy) at 0.03 mL/kg, computed tomography (CT) was performed with Optima 64 and Revolution 256-slice spiral CT scanners (GE Healthcare), and magnetic resonance imaging (MRI) was performed with a MAGNETOM Skyra 3.0T magnetic resonance scanner (Siemens Healthineers, Erlangen, Germany).

A standardized CMR examination was performed using a 3.0 T magnetic resonance imaging (MRI) scanner (MAGNETOM Skyra 3.0T with TIM and DOT technology, Siemens, Oakville, ON, Canada) with a phased-array cardiac coil and retrospective electrocardiographic gating, with images obtained on end-expiration breath-holds. A short-axis stack and long-axis views (2- and 4-chamber) were acquired to analyze left ventricular (LV) and RV mass, dimensions, and function. All scans were performed on the same Siemens 3T Skyra. All CMR volumetric endpoints were measured by a single reader blinded to other clinical data, using commercially available software with particular care to exclude papillary muscles and artifact (CVi42 Version 5.1; Circle Imaging, Calgary, AB). Ventricular mass and function were determined via a manual tracing of the endocardial and epicardial borders.¹⁴ (link)

Preoperative MRI was performed on two 3.0-T MRI systems (MAGNETOM Skyra 3.0 T, Siemens Healthineers, Erlangen, Germany). The entire prostate gland and seminal vesicles were imaged on coronal, sagittal, and axial slices using T2WI, DWI, and DCE. T2WI was completed with a fast-recovery fast-spin-echo (FR-FSE) sequence [repetition time (TR)/echo time (TE), 7,120 ms/89 ms; number of excitations, 2; slice thickness, 3 mm; spacing, 1 mm; matrix 324×320]. T1WI was completed with a fast spoiled gradient-echo (FSPGR) sequence (TR/TE, 231 ms/2.5 ms; slice thickness, 5.5 mm; spacing, 1 mm; matrix 204×320). DWI was completed with a readout-segmented echo-planar imaging (RS-EPI)-DWI sequence (TR/TE, 4,670 ms/63 ms; field of view, 182×240 mm; slice thickness, 3 mm; spacing, 1 mm; matrix 88×116) with identical slice locations to those of transverse T2WI, and b values of 50, 1,000, and 1,500 s/mm². The apparent diffusion coefficient (ADC) value was calculated using the workstation with b values of 50 and 1,000 s/mm², and an ADC map was also generated.

Relative NRF2 score calculated based on the cumulative expression of classic NRF2 target genes was used as our NRF2 activity index (Liu et al, 2010 (link); DeNicola et al, 2015 (link)). In this study, the NRF2 score was calculated based on seven classic NRF2 target genes: GCLC, GCLM, NQO1, HMOX-1, Aldoketo Reductase family 1 member C1 (AKR1C1), C2 (AKR1C2) and C3 (AKR1C3) (Tebay et al, 2015 (link); MacLeod et al, 2016 (link)). The expression of each gene was normalised by GAPDH and the results for these seven genes were added together for HCC and AT, respectively, to obtain total relative NRF2 scores. The following clinical data were collected to explore the potential correlation with relative NRF2 score in HCC: serum α-fetoprotein (AFP) levels, longest diameters measured with computed tomography (CT, Discovery CT750 HD, GE Health-Care Biosciences, Pittsburgh, PA, USA) or magnetic resonance imaging (MRI, MAGNETOM Skyra 3.0T, Siemens Healthineers, Erlangen, Germany) in mm, Child–Pugh classification (A/B/C), Barcelona Clinic Liver Cancer (BCLC) classification (0, A, B, C, D), tumour pathological differentiated degrees and plasma biochemical indexes, including alanine transaminase, aspartate transaminase, albumin, prothrombin time, prothrombin activity, prothrombin time activity of pre- and post-operation.