Unless otherwise specified, EBV lytic induction in P3HR-1, Akata and AGSiZ cells were induced with 400 nM 4-Hydroxytamoxifen (4HT) (Sigma Aldrich) for 24h, 15 μg/ml anti-IgG crosslinking (Agilent) for 48h and 5 μg/ml doxycycline (Sigma Aldrich) for 24h, respectively. Inhibition of NLRP3 inflammasome in lytic-induced BILF1-depleted P3HR-1 cells was achieved by the addition of 10 μM MCC950 (InvivoGen) simultaneously with the EBV lytic inducer 4HT for 24h. NLRP3 inflammasome activation was induced by the potassium ionophore, Nigericin (InvivoGen) at 10 μM for 4h.
Anti igg crosslinking
Manufactured by Agilent Technologies
The Anti-IgG crosslinking product is a laboratory equipment designed for the crosslinking of immunoglobulin G (IgG) antibodies. It facilitates the formation of covalent bonds between IgG molecules, which can be useful in various biochemical and immunological applications.
Automatically generated - may contain errors
2 protocols using anti igg crosslinking
1
EBV Lytic Induction and NLRP3 Inflammasome Modulation
2
EBV Lytic Induction and NLRP3 Inflammasome Modulation
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