Mouse anti chop

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Mouse anti-CHOP is an antibody that recognizes the CHOP protein. CHOP is a transcription factor that is involved in the regulation of cellular stress responses.

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Lab products found in correlation

Anti eif2α, by Santa Cruz Biotechnology (1 mentions) Mouse anti kdel, by Enzo Life Sciences (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Actinomycin d, by Merck Group (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Hrp conjugated goat anti rabbit and goat anti mouse secondary antibodies, by Jackson ImmunoResearch (1 mentions) Mouse anti cd68, by Santa Cruz Biotechnology (1 mentions) Mouse anti cd206, by Santa Cruz Biotechnology (1 mentions) Prolong gold antifade reagent containing dapi, by Cell Signaling Technology (1 mentions) Alexa fluor 488, by Abcam (1 mentions) Mouse anti inos, by Santa Cruz Biotechnology (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions)

Close spelling variants of this product

Mouse monoclonal anti-CHOP Anti-CHOP

3 protocols using mouse anti chop

Protein Quantification and Antibody Detection

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We used mouse anti-CHOP (Santa Cruz Biotechnology sc-7351), anti-eIF2α (Santa Cruz Biotechnology sc-200, sc-11386); rabbit anti-GAPDH (Cell Signaling Technology 2118S), rabbit anti-MYC (Immunology Consultants catalog RMYC-45A-Z), mouse anti-KDEL (Enzo Life Sciences, catalog ADI-SPA-827), mouse anti–α-tubulin (MilliporeSigma, catalog T5168), and rabbit anti-TG and anti-BiP as previously described (13 (link)). HRP-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies were from Jackson ImmunoResearch (catalog 111-035-144 and 115-035-174, respectively). TUN and actinomycin D were from MilliporeSigma.

Morishita Y., Kellogg A.P., Larkin D., Chen W., Vadrevu S., Satin L., Liu M, & Arvan P. (2021). Cell death–associated lipid droplet protein CIDE-A is a noncanonical marker of endoplasmic reticulum stress. JCI Insight, 6(7), e143980.

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Immunofluorescence Assay for Cellular Characterization

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The immunofluorescence assay was performed with modifications, as previously described [45 (link)]. Briefly, cryosections and BMDM were washed with tris-buffered saline (TBS) and fixed in 4% paraformaldehyde (PFA) for 10 min. After washing, the samples were permeabilized with TBST (0.2% Triton X-100 in TBS) for 10 min and washed three times with TBS for 5 min. Samples were blocked using 2% BSA and incubated at 4°C overnight with primary antibodies, including rabbit anti-Nogo-A (Abcam, catalog no. ab62024), mouse anti-CD68 (Santa Cruz Biotechnology, catalog no. ab955), mouse anti-iNOS (Santa Cruz Biotechnology, ab49999), mouse anti-CD206 (Santa Cruz Biotechnology, catalog no. sc-58986), mouse anti-CHOP (Santa Cruz Biotechnology, sc-71136), and mouse anti-calnexin (Novus Biologicals, catalog no. NB300518). After three washes with TBS for 5 min, samples were incubated with secondary antibodies (donkey anti-mouse immunoglobulins (Alexa Fluor 488, Abcam, catalog no. ab150105) and donkey anti-rabbit immunoglobulins (Alexa Fluor 555, Abcam, catalog no. ab150066) for 1 h in the dark. The samples were mounted using ProLong™ Gold Antifade reagent containing DAPI to visualize the nuclei (Cell Signaling Technology, catalog no. 8961s) and were analyzed by confocal microscopy (ZEISS).

Ullah H.M., Elfadl A.K., Park S., Kim Y.D., Chung M.J., Son J.Y., Yun H.H., Park J.M., Yim J.H., Jung S.J., Choi Y.C., Shin J.H., Kim D.S., Park J.K, & Jeong K.S. (2021). Nogo-A Is Critical for Pro-Inflammatory Gene Regulation in Myocytes and Macrophages. Cells, 10(2), 282.

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Western Blot Analysis of Lens Proteins

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Lens tissues were ground and lysed in radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitor cocktails (A32961; Thermo Fisher Scientific). Samples were separated on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred to a polyvinylidene difluoride (PVDF) membrane (162-0177; Bio-Rad Laboratories, Hercules, CA, USA). After blocking with 5% non-fat milk, the membrane was incubated with a primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibody (1:5000) for 1 hour at room temperature. The Immobilon Western Chemiluminescent HRP substrate (MilliporeSigma, Burlington, MA, USA) was used to detect specific antibody binding. These signals were captured using a Syngene G:BOX Chemi XT16 imaging device (Syngene, Cambridge, UK). Primary antibodies included goat anti-JAM-C (1:500, AF1189; R&D Systems), rabbit anti-BiP (1:1000, C50B12; Cell Signaling Technology), mouse anti-CHOP (1:1000, sc-7315; Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-α-tubulin (1:1000, RM2007; Beijing Ray Antibody Biotech, Beijing, China), and mouse anti-β-actin (1:1000, RM2001; RayBiotech).

Li J., Tan X., Sun Q., Li X., Chen R, & Luo L. (2022). Deficiency of Jamc Leads to Congenital Nuclear Cataract and Activates the Unfolded Protein Response in Mouse Lenses. Investigative Ophthalmology & Visual Science, 63(10), 1.

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