Anti vegfr2 clone 55b11

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-VEGFR2 (clone 55B11) is a monoclonal antibody product from Cell Signaling Technology. It is designed to detect vascular endothelial growth factor receptor 2 (VEGFR2).

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Lab products found in correlation

Anti active caspase 3, by Cell Signaling Technology (1 mentions) Anti vegfr 1, by Santa Cruz Biotechnology (1 mentions) Anti actin, by Merck Group (1 mentions) Anti phospho vegfr1 tyr1213, by Merck Group (1 mentions) Anti actin, by Cell Signaling Technology (1 mentions) Anti tubulin, by Santa Cruz Biotechnology (1 mentions) Streptavidin horse radish peroxidase conjugate, by Merck Group (1 mentions)

2 protocols using anti vegfr2 clone 55b11

Immunoblotting Assay for VEGFR1 and VEGFR2

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Immunoblotting experiments were performed as previously described.^{21 (link)} The antibodies used were: anti-sVEGFR1-i13 polyclonal antibody, generated against a peptide mapping in the unique C-terminus,^{14 (link)} anti-VEGFR2 (clone 55B11), anti-actin and anti-active caspase-3 from Cell signalling. Anti-tubulin was from Santa Cruz, anti-actin from Sigma-Aldrich, anti-phospho-VEGFR1-Tyr1213 from Millipore and anti-phospho-VEGFR2-Tyr1214 from Invitrogen. Antibody against total β1 integrin (serum 227) was kindly provided by Dr Albiges-Rizo (Institute For Advanced Biosciences, Grenoble). The other antibodies used for Proximity Ligation Assay and immunohistochemistry were: mouse anti-β1 integrin (4B7R) and anti-VEGFR2 (4B4) antibodies from ThermoFisher Scientific, rabbit anti-VEGFR2 from Sigma-Aldrich and anti-VEGFR1 (Santa Cruz).

Abou Faycal C., Brambilla E., Agorreta J., Lepeltier N., Jacquet T., Lemaître N., Emadali A., Lucas A., Lacal P.M., Montuenga L., Pio R., Gazzeri S, & Eymin B. (2018). The sVEGFR1-i13 splice variant regulates a β1 integrin/VEGFR autocrine loop involved in the progression and the response to anti-angiogenic therapies of squamous cell lung carcinoma. British Journal of Cancer, 118(12), 1596-1608.

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Monoclonal Antibody Production and Characterization

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The FSHR-highly specific monoclonal antibody 323 was produced in ascites and purified as described [25 (link)]. Biotinylated goat anti-mouse IgG (Fc specific) and streptavidin-horseradish peroxidase conjugate were purchased from Sigma-Aldrich, Saint Quentin Fallavier, France. Mouse anti-human CD34 (clone QBEnd 10) and mouse anti-human podoplanin (clone D2-40) monoclonal antibodies were purchased from Dako A/S, Glostrup, Denmark. Rabbit monoclonal anti-HER2/neu (clone 4B5), rabbit monoclonal anti-ER (sp1), and rabbit monoclonal anti-PR (IE2) were from Ventana Medical Systems Inc., Oro Valley, AZ, USA. Rabbit monoclonal antibody anti VEGFR2 (clone 55B11) was from Cell Signaling Technology, Danvers, MA, USA.

Planeix F., Siraj M.A., Bidard F.C., Robin B., Pichon C., Sastre-Garau X., Antoine M, & Ghinea N. (2015). Endothelial follicle-stimulating hormone receptor expression in invasive breast cancer and vascular remodeling at tumor periphery. Journal of Experimental & Clinical Cancer Research : CR, 34(1), 12.

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