ATAD2 expression was analyzed on paraffin-embedded specimens from 129 patients. Tissue sections of 4-μm thickness were deparaffinized in xylene and dehydrated before antigen retrieval for 5 min with an autoclave. Hydrogen peroxide (0.3%) was used to block endogenous peroxidase activity; nonspecific immunoglobulin binding sites were blocked by normal goat serum for 30 min at 37°C. Tissue sections were incubated with anti-ATAD2 (1:200, Sigma) overnight at 4°C. Biotinylated goat anti-rabbit immunoglobulin G was used as a secondary antibody (1:300, Sigma). After washing, the sections were incubated with streptavidin-biotin conjugated with HRP for 30 min, and the peroxidase reaction was developed with 3, 3′-diaminobenzidine tetrahydrochloride (DAB).
Biotinylated goat anti rabbit immunoglobulin g
Manufactured by Merck Group
Biotinylated goat anti-rabbit immunoglobulin G is a laboratory reagent used for the detection and quantification of rabbit immunoglobulin G (IgG) in various immunoassay techniques. It is a secondary antibody conjugated with biotin, which can bind to streptavidin or avidin-based detection systems, enabling the amplification and visualization of the target rabbit IgG.
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Lab products found in correlation
Dextran sulfate sodium (dss), by Merck Group (1 mentions)
3 3 diaminobenzidine (dab), by Merck Group (1 mentions)
Proteinase k, by Merck Group (1 mentions)
1 2 dimethylhydrazine, by Merck Group (1 mentions)
Tnf α, by BioLegend (1 mentions)
Streptavidin peroxidase, by Merck Group (1 mentions)
Bcl2 associated x (bax), by BioLegend (1 mentions)
Caspase 3, by BioLegend (1 mentions)
Raptinal, by Merck Group (1 mentions)
Interleukin 6 (il 6), by BioLegend (1 mentions)
2 protocols using biotinylated goat anti rabbit immunoglobulin g
1
Immunohistochemical Analysis of ATAD2 Expression
2
Colorectal Cancer Pathogenesis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Entire chemicals have been an analytical reagent brand. Raptinal (97% pure by HPLC), Dextran sulfate sodium (DSS), 1,2 dimethylhydrazine (DMH), streptavidin peroxidase, biotinylated goat anti-rabbit immunoglobulin G, proteinase K and 3,3'-diaminobenzidine (DAB) been obtained by Sigma Aldrich Chemical Co.(St. Louis, MO, USA). Rabbit anti-rat Bcl-2, p53, caspase-3, Bax, IL-6, TNF-α, and Ki-67 have been purchased through BioLegend (San Diego, California, USA). The apoptotic kit has been procured by Takara Bio Inc (Kusatsu, Japan).
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