Serum levels of cytokine TGF-β1 were assessed in the patients and the controls by ELISA set procured from BD Biosciences (Becton and Dickinson, New Jersey, USA). The values of unknown samples were calculated by log-log regression.
Elisa set
Manufactured by BD
Sourced in United States, United Kingdom
The ELISA set is a laboratory equipment used for the Enzyme-Linked Immunosorbent Assay (ELISA) technique. The core function of the ELISA set is to detect and quantify specific proteins, hormones, antibodies, or other biomolecules in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Biotin rat anti mouse ige, by BD (1 mentions)
Synergy ht plate reader, by Agilent Technologies (1 mentions)
Immunospot professional software package version 5, by Cellular Technology (1 mentions)
Immunospot s4, by Cellular Technology (1 mentions)
Human ifn γ elispotpro kit, by Mabtech (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Human anti virus response panel 13 plex, by BioLegend (1 mentions)
10 protocols using elisa set
1
Serum TGF-β1 Levels Assessment
2
Anti-OVA Antibodies and Cytokines Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The anti-OVA IgE, IgG1, and IgG2a antibodies levels in sera obtained before, during, and after nC60-treatment were detected by ELISA (ELISA kits from BD, USA) according to the manufacturer’s protocol. OVA was used for coating the plates. Mouse anti-ovalbumin IgE mAb (AbD Serotec, UK) and biotin rat anti-mouse IgE (BD) were used for detection anti-OVA mouse IgE. These components were used to construct the calibration curve and then to analyze sera.
The spleens were taken after the last allergen application, and levels of IL-4, IL-5, and IL-12 (p40) in supernatants of OVA-stimulated splenocytes were determined by using ELISA [Duo-Set from R&D Systems (UK) and ELISA set from BD (USA)] according to the manufacturer’s protocol.
The spleens were taken after the last allergen application, and levels of IL-4, IL-5, and IL-12 (p40) in supernatants of OVA-stimulated splenocytes were determined by using ELISA [Duo-Set from R&D Systems (UK) and ELISA set from BD (USA)] according to the manufacturer’s protocol.
3
BMDM TNFα Expression Quantification
4
Macrophage Immunostimulation by CBP1S
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to measure the immunostimulating activity of isolated CBP1S, peritoneal macrophages were harvested from 5% thioglycollate medium (TG)-injected BALB/c mice as previously described (Salki et al., 1988 (link)). The collected macrophages were suspended in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) and plated at 2.5 × 105 cells/well onto 96-well culture plates. After 2 h, various concentrations of CBP1S were added to the cells and incubated at 37 °C for 24 h. The levels of interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α were measured in the culture supernatant using the respective sandwich enzyme-linked immunosorbent assay (ELISA) set (BD bioscience, San Diego, CA, USA).
5
Evaluating NK Cell Cytotoxicity in Yac-1 Lymphoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Yac-1 is a Moloney murine leukemia virus-induced lymphoma that does not express major histocompatibility complex (MHC)-I and is sensitive to lysis by NK cells (Kiessling, Klein, & Wigzell, 1975 (link)). Therefore, NK-mediated cytotoxicity was determined in Yac-1 and primary cultured splenocytes from sample-treated animals (Yoon et al., 1998 (link)). Briefly, three BALB/c mice per group were administered CBP1S intravenously (1000 μg/mouse), and their splenocytes were harvested three days after treatment. Single cell suspensions of splenocytes were added to the Yac-1 cells (1 × 104 cells/well) to obtain effector cell to target cell (E/T) ratios of 100:1, 50:1 and 25:1 in round bottomed 96 well plates, and co-cultured for 24 h. We then measured the levels of interferon-gamma (IFN-γ) and granzyme released from activated NK cells using the respective ELISA set (BD bioscience, San Diego, CA, USA).
6
Multiplex Cytokine Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Secreted INF-γ, IL-4, IL-13, IL-17A, IL-22, and IL-10 were quantified in culture supernatants by using the human ProcartaPlex Multiplex Immunoassay (eBiosciences Inc., San Diego, CA, USA). Concentration of each analyte was detected using the MAGPIX instrument (Luminex Corp., Austin, TX, USA) in the facilities of the Scientific and Technological Centres of the University of Barcelona (CCiT-UB, Barcelona, Spain). Data were analyzed with xPONENT® 4.2 software (Luminex Corp.). Secreted TGF-β was measured by an enzyme linked immunosorbent assay (ELISA) set (BD Biosciences, San Jose, CA, USA).
7
IFNγ Secretion Assay of TILs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IFNγ secretion of TILs was measured by ELISPOT using Human IFNγ ELISpotPRO kit (MABTECH, Catalog number 3420–2APW-10) following the manufacturer’s instruction. Briefly, 5 × 104 resting TILs were co-cultured with 2 × 104 peptide-pulsed C1R A02:01 cells at 37°C for 20 hours in a 96-well plate. Spots were captured and analyzed by an automated ELISPOT reader, ImmunoSPOT S4 (Cellular Technology Ltd, Shaker Heights, OH), and analyzed with the ImmunoSpot Professional Software package, Version 5.1 (Cellular Technology Ltd).
The amount of secreted IFNγ, IL-2, and TNFα in the supernatant of T cells were quantified with ELISA set (BD Biosciences, Catalog number 555142, 555190, 555212). Briefly, 5 × 104 resting KIAA1429D1358E TCR-T cells were co-cultured with 2 × 104 peptide-pulsed C1R A02:01 cells at 37°C for 20 hours in a 96-well plate. The supernatant was collected, and the concentration of each protein was measured according to the manufacturer’s instruction.
The amount of secreted IFNγ, IL-2, and TNFα in the supernatant of T cells were quantified with ELISA set (BD Biosciences, Catalog number 555142, 555190, 555212). Briefly, 5 × 104 resting KIAA1429D1358E TCR-T cells were co-cultured with 2 × 104 peptide-pulsed C1R A02:01 cells at 37°C for 20 hours in a 96-well plate. The supernatant was collected, and the concentration of each protein was measured according to the manufacturer’s instruction.
8
Splenocyte Cytokine Production Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The spleens were taken after the last allergen application aseptically. Splenocytes (5×106 cells/well) were plated in 12-well plates and incubated for 72 h in RPMI 1640 medium (GIBCO, USA) containing 10% FBS (HyClone, USA), gentamicin (800 μg/mL), 50 μM β-ME at 37°C in a 5% CO2 humidified incubator. Splenocytes were stimulated or non-stimulated with OVA (4 μg/mL). The levels of IL-4, IL-5, IL-17, IFN-γ, and IL-12 (p40) in supernatants of OVA-stimulated or non-stimulated splenocytes were determined by ELISA (Duo-Set, R&D Systems, UK; ELISA set, BD, USA) according to the manufacturer’s protocol.
9
Complement System Activation Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to measure C3a, C5a, and C5b-9 concentrations, commercial ELISA kits from BD Bioscience were used and measured in the InflaRx Laboratory (Jena, Germany). C3a and C5a ELISAs are “ready-to-use” kits and are characterized by BD Bioscience. These ELISAs were performed according to the manufacturers' instructions. The C5b-9 ELISA was validated by the InflaRx Laboratory based on the ELISA set (including coating and detection antibody and C5b-9 standard) provided by BD Bioscience. The C3a, C5a, and C5b-9 ELISAs were used for human biological samples with the reference values in activated plasma from healthy humans of 530 ng/mL for C3a, 15 ng/mL for C5a, and 74 ng/mL for C5b-9.
10
Early LPS-induced Signaling in THP-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the assessments of early LPS-stimulated cellular response, 3×105 THP-1 cells per well were plated in a 48-well format and treated with 100 ng/mL of Lipopolysaccharides (LPS) derived from Escherichia coli O111:B4 (Sigma, L3012) for 0, 10, and 20 minutes. The cells were subjected to immunoblotting analysis to reveal the phosphorylation states of IκBα and NF-κB p65 subunit. For measuring the LPS-induced production of proinflammatory cytokine, 1.5×105 THP-1 cells per well were plated in a 96-well format using U-bottom plate and after stimulation with 100 ng/mL LPS for 6 hrs, the supernatants were collected for cytokine determinations using ELISA Set (BD Biosciences) or Human Anti-Virus Response Panel (13-plex) (Biolegend, 740390) following the manufacturers’ instructions, and the cells for mRNA measurements using RT-qPCR.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!