Tetracycline

Manufactured by Coolaber

Sourced in China

Tetracycline is a broad-spectrum antibiotic used in laboratory settings. It is a yellow, crystalline powder that is soluble in water and alcohol. Tetracycline inhibits the growth of a wide range of bacteria by interfering with their protein synthesis.

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2 protocols using tetracycline

Antibiotic Susceptibility Testing Protocol

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Tetracycline, oxyTetracycline, chlorTetracycline, doxycycline, ethidium bromide (EB), Hoechst33342 stain, cefoperazone, cefazolin, streptomycin, ampicillin, roxithromycin, chloramphenicol, rifampicin, norfloxacin, deoxycholate, sodium cholate, ofloxacin, doxorubicin, daunorubicin, acridine flavin, and quinine were purchased from Coolaber (Beijing, China), and ortho-vanadate was purchased from Sigma-Aldrich (St. Louis, MO, United States). Carbonylcyanide m-chlorophenylhydrazone (CCCP) and reserpine were obtained from MedChemexpress (Monmouth Junction, NJ, United States). Drug-sensitive paper was purchased from Hangzhou Microbial Preparation (Hangzhou, China).

Feng Z., Liu D., Wang L., Wang Y., Zang Z., Liu Z., Song B., Gu L., Fan Z., Yang S., Chen J, & Cui Y. (2020). A Putative Efflux Transporter of the ABC Family, YbhFSR, in Escherichia coli Functions in Tetracycline Efflux and Na+(Li+)/H+ Transport. Frontiers in Microbiology, 11, 556.

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Bacterial Resistance Mechanisms Assay

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Tetracycline, oxyTetracycline, doxycycline, rifampicin, norfloxacin, cefminox, cefazolin, daunorubicin, doxorubicin, streptomycin, roxithromycin, deoxycholate, chloramphenicol, ofloxacin, acridine flavin, sodium cholate, ampicillin, quinine, Hoechst 33342, and ethidium bromide were from Coolaber (China). Ortho-vanadate was from Sigma-Aldrich (USA). Carbonylcyanide m-chlorophenylhydrazone (CCCP) and reserpine were from MedChemexpress (Monmouth Junction, USA). norfloxacin disks were from Hangzhou Microbial Preparation (China). Gel extraction kits were from ThermoScientific. Wizard SV Gel and PCR Clean-UP System were from Promega (Promega, USA). DH5α competent cells were from Tiangen Biochemical Technology Co., Ltd. (China). Competent cell preparation kits were from TaKaRa (Japan).

Feng Z., Liu D., Liu Z., Liang Y., Wang Y., Liu Q., Liu Z., Zang Z, & Cui Y. (2020). Cloning and Functional Characterization of Putative Escherichia coli ABC Multidrug Efflux Transporter YddA. Journal of Microbiology and Biotechnology, 30(7), 982-995.

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