Anti e47 sc 763

Manufactured by Santa Cruz Biotechnology

Anti-E47 (sc-763) is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that targets the E47 protein. The core function of this product is to enable the detection and study of the E47 protein in various research applications.

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Lab products found in correlation

Cyquant cell proliferation assay kit, by Thermo Fisher Scientific (1 mentions) Infrared dye conjugated secondary antibody, by LI COR (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Antibody 9661, by Cell Signaling Technology (1 mentions) Anti tubulin, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Cytiva (1 mentions) Ecl plus detection reagent, by Cytiva (1 mentions) Anti flag f3165, by Merck Group (1 mentions) Anti gapdh sc 32233, by Santa Cruz Biotechnology (1 mentions) Anti gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Anti c myc sc 764, by Santa Cruz Biotechnology (1 mentions) Anti histone h3 ab1791, by Abcam (1 mentions) Hybond, by Cytiva (1 mentions)

2 protocols using anti e47 sc 763

Cell Proliferation and Apoptosis Assays

Cited 1 time

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We analyzed growth by counting cells directly by hemavet and by CyQuant Cell Proliferation Assay kit (Life Technologies, Grand Island, New York). Western blot analysis was done with anti-E47 (sc-763, Santa Cruz Biotechnology), anti-Lmo2 (monoclonal antibody provided by Dr. Ron Levy, Stanford), and anti-tubulin (sc-55529, Santa Cruz Biotechnology) antibodies. FACS analyses were done using anti-CD4 (FITC-conjugated Rat anti-mouse, 553650, BD Pharmingen) and anti-CD8 (PE Rat anti-mouse, 55032, BD Pharmingen). Sorting was done using FITC-conjugated anti-hCD25 (347643, BD Biosciences). Quantification of proteins by Western blotting was done using 680nm and 800nm infrared dye-conjugated secondary antibodies (LI-COR) on the Odyssey machine. Cleaved caspase 3 was analyzed by FACS using antibody 9661 per manufacturer's instructions (Cell Signaling Technology). FACS data was imported into Flojo for further analysis. Bromodeoxyuridine (BrdU) incorporation was analyzed per manufacturer's instructions (BD Biosciences, San Jose, California) as previously described (29 (link)). Intracellular cleaved caspase 3 staining was performed using the BD Cytoperm/ Cytofix kit (BD Biosciences, San Jose, California). Statistical analyses were done using GraphPad Prism 6.0.

Goodings C., Tripathi R., Cleveland S.M., Elliott N., Guo Y., Shyr Y, & Davé U.P. (2014). Enforced expression of E47 has differential effects on Lmo2-induced T-cell leukemias. Leukemia research, 39(1), 100-109.

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Western Blot Protocol for Protein Detection

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Cell extracts or immunoprecipitated proteins were diluted in SDS sample buffer and boiled for 5 minutes. Protein was separated on either 10% or 8% SDS‐Page gels and transferred to PVDF Transfer Membrane Hybond^™ (Amersham Bioscience). Membranes were saturated with 5% non‐fat dry milk in PBS containing 0.1% Tween20 (PBST) for 1 hour at RT. The antibodies (anti ‐c‐MYC sc764, anti‐GFP sc9996, anti‐GST sc9996, anti‐E47 sc763, anti‐GAPDH sc‐32233 were from Santa Cruz Biotechnology; anti‐Flag F3165 was from Millipore; anti STRA8 Ab49405, anti‐SOHLH1 Ab41520 and anti‐HISTONE H3 Ab1791 were from Abcam; anti c‐KIT gently provided by Prof. S. Dolci) were diluted in PBST buffer and added to the PVDF membrane for 1 hour at RT or overnight at 4°C followed by incubation with the appropriate horseradish peroxidase‐conjugated secondary antibodies (Amersham Bioscience) for 45 minutes at RT. The STRA8 and SOHLH1 immunoprecipitated protein were detected after incubation with the peroxidase‐conjugated anti‐rabbit IgG light chain specific (Jackson ImmunoReasearch). All proteins were detected with ECL plus detection reagents (Amersham Bioscience) and visualized by chemiluminescence.

Desimio M.G., Cesari E., Sorrenti M., De Felici M, & Farini D. (2020). Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c‐KIT expression in vitro. Journal of Cellular and Molecular Medicine, 25(1), 383-396.

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