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Wl0003b

Manufactured by Wanlei
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Sourced in China

The WL0003b is a laboratory equipment designed for general scientific applications. It features precise temperature control and measurement functionality. The device's core function is to provide a controlled environment for various experimental procedures.

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Lab products found in correlation

Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Anti mmp2, by Wanlei (1 mentions) Anti p akt, by Wanlei (1 mentions) Anti akt, by Wanlei (1 mentions) Anti vimentin, by Wanlei (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Ab92726, by Abcam (1 mentions) Annexin 5, by BD (1 mentions) Ap0684, by ABclonal (1 mentions) Wl02891, by Wanlei (1 mentions) Ac026, by ABclonal (1 mentions) Zb 2308, by ZSGB-BIO (1 mentions) As061, by ABclonal (1 mentions) Ap0140, by ABclonal (1 mentions) Ac011, by ABclonal (1 mentions) Ae003, by ABclonal (1 mentions) Ae020, by ABclonal (1 mentions) Ae012, by ABclonal (1 mentions) Ac005, by ABclonal (1 mentions)

3 protocols using wl0003b

1

Western Blot Analysis of Stem Cell Markers

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Cell samples were lysed in NP40‐containing buffer with PMSF, protease inhibitors, and phosphatase inhibitors. Individual cell lysate samples (30 µg/lane) were separated via SDS‐PAGE on a 12% resolving gel and electro‐transferred to polyvinylidene difluoride membranes (Millipore, Hong Kong). The membranes were blocked with 5% non‐fat dry milk in TBST and incubated overnight at 4°C with primary antibodies, including anti‐human LIPH (1:500, Proteintech, Wuhan, China), anti‐GAPDH (Cell Signaling Technology, Danvers, MA), anti‐CD44 (60224‐1‐Ig), anti‐CD24 (10600‐1‐AP), anti‐Oct4 (11263‐1‐AP), anti‐Sox2 (11064‐1‐AP), anti‐vimentin (WL01960), anti‐MMP2 (WL01579a, WanleiBio, China), anti‐FAK p397 (ab24781, Abcam, UK), anti‐Integrin‐ß3 (WL02735b, WanleiBio), anti‐p‐AKT (WLp001, WanleiBio), anti‐AKT (WL0003b, WanleiBio), anti‐RAS (WL0257, WanleiBio), anti‐CAPN2 (abs137284), anti‐Paxillin (WL00415, WanleiBio), anti‐Vinculin (abs131199), and anti‐Tubulin‐α (2144s, CST, USA). The bound antibodies were detected with horseradish peroxidase (HRP)‐conjugated secondary antibodies (1:10 000). The immunoblotting signals were visualized using enhanced chemiluminescent reagents. The ratios of target proteins to control GAPDH were determined by densitometric analysis using the ImageJ software application (NIH, Bethesda, MD, USA).
Zhang Y., Zhu X., Qiao X., Gu X., Xue J., Han Y., Sun L., Cui M, & Liu C. (2020). LIPH promotes metastasis by enriching stem‐like cells in triple‐negative breast cancer. Journal of Cellular and Molecular Medicine, 24(16), 9125-9134.
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2

Antibody-Based Protein Expression Analysis

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Antibodies for TSG101 (14497-1-AP, 1:1,000), HSP70 (10995-1-AP, 1:1,000), Angiopoietin 1 (23302-1-AP, 1:1,000), Angiopoietin 2 (24613-1-AP, 1:000), PHLPP2 (25244-1-AP, 1:1,000), and p-Akt (66444-1-Ig, 1:3,000) were purchased from Sanying (China). Antibodies for Akt (WL0003b, 1:500), eNOS (WL0992a, 1:500), PTEN (WL01901, 1:500), and CD63 (WL02549, 1:1,000) were purchased from Wanlei (China). Antibodies for GRP94 (D120724, 1:1,000) were purchased from BBI (China). Antibodies for CD9 (ab92726, 1:2,000) were purchased from Abcam (USA). Antibodies for GAPDH (70-ab011-040, 1:2,000) served as a reference protein probe, and HRP-conjugated Goat Anti-Rabbit IgG (70-GAR007, 1:1,000) was purchased from Multisciences (China). Cy3-AffiniPure Goat Anti-Rabbit IgG was purchased from Yeasen (China). Annexin V was purchased from BD Biosciences (USA).
Wang Y., Lu J., Chen L., Bian H., Hu J., Li D., Xia C, & Xu H. (2020). Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC. Molecular Therapy. Nucleic Acids, 20, 421-437.
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3

Immunoblotting Reagents for Protein Analysis

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Monoclonal anti-Actin Beta (ACTB) antibody (AC026, ABclonal Technology, Wuhan, China), monoclonal anti-GFP antibody (AE012, ABclonal Technology), monoclonal anti-RFP antibody (AE020, ABclonal Technology), monoclonal anti-His antibody (AE003, ABclonal Technology), polyclonal anti-phospho-AKT antibodies (AP0140, ABclonal Technology), polyclonal anti-phospho-FOXO antibodies (AP0684, ABclonal Technology), mouse control IgG (AC011, ABclonal Technology), rabbit control IgG (AC005, ABclonal Technology), Horseradish Peroxidase (HRP)-conjugated goat anti-rabbit IgG light chain (AS061, ABclonal Technology), polyclonal anti-AKT antibodies (WL0003b, Wanleibio, Shenyang, China), polyclonal anti-FOXO antibodies (WL02891, Wanleibio), Alkaline phosphatase (AP)-conjugated horse anti-mouse IgG (ZB2310, ZSGB-BIO, Beijing, China), and AP-conjugated goat anti-rabbit IgG (ZB2308, ZSGB-BIO).
Di Y.Q., Zhao Y.M., Jin K.Y, & Zhao X.F. (2021). Subunit P60 of phosphatidylinositol 3-kinase promotes cell proliferation or apoptosis depending on its phosphorylation status. PLoS Genetics, 17(4), e1009514.
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