Male wild-type C57/BL mice, 6 to 8 weeks old, were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd (Beijing, China). IL-17a-/- (IL-17 KO) mice in a C57BL/6 background were provided by Dr. Iwakura (University of Tokyo, Tokyo, Japan) as previously described.[17 (link)] The animals were housed in individual ventilated cages (IVC) under standard laboratory conditions with a 12/12h light/dark cycle. The full system was kept in an environmentally controlled room at (22 ± 1)oC and (55 ± 5)% humidity. Clean food and water were provided ad libitum. All mice were maintained in the Beijing Institute of Respiratory Medicine. The following five groups were set up: control, IPA, COPD, COPD+IPA, and IL-17 KO COPD+IPA groups.
C57 bl mice
Manufactured by Charles River Laboratories
Sourced in China
C57/BL mice are an inbred strain of laboratory mice developed by The Jackson Laboratory. They are a widely used model organism in biomedical research, serving as a general-purpose strain for a variety of experimental applications.
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Lab products found in correlation
8 protocols using c57 bl mice
1
Experimental Mouse Model of COPD and IPA
2
Optimizing Stem Cell Therapy for Parkinson's Disease
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Human umbilical cords were obtained from healthy women who had childbirth in our hospital. All samples obtained were provided by these women who agreed and signed consent forms. C57BL mice (6–8 weeks) were ordered from Beijing Vital River Laboratory Animal Technology Co. Collection of samples and handling animals were all approved by the Ethics Committee of Neurology Department of General Hospital of Jinan Military Region. All experiments were reviewed by the Ethics Committee of Neurology Department of General Hospital of Jinan Military Region. Adenovirus expressing green fluorescent protein gene (Ad-GFP) was provided by Beckman Medical Instruments, USA. Recombinant adenovirus carrying FGF-20 gene (Ad-FGF-20) was constructed in our lab.
Consumables such as F12 culture medium, pipettes, and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were all ordered from Sigma (USA). Fetal calf serum was ordered from HyClone Company (USA). All ELISA reagent kits were ordered from IBL (Germany). All antibodies were ordered from Abcam (England). Chromogenic substrate kit was ordered from Shanghai Ruichi Biotechnology Co., Ltd. (Shanghai, China).
Consumables such as F12 culture medium, pipettes, and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were all ordered from Sigma (USA). Fetal calf serum was ordered from HyClone Company (USA). All ELISA reagent kits were ordered from IBL (Germany). All antibodies were ordered from Abcam (England). Chromogenic substrate kit was ordered from Shanghai Ruichi Biotechnology Co., Ltd. (Shanghai, China).
3
Pharmacokinetics and Cardioprotective Effects
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Sprague–Dawley (SD) male rats (200 ± 20 g), male and female C57/BL mice (18 ± 2 g), and male and female Balb/C mice (20 ± 2 g) were all provided by Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). The animals were housed in a specific-pathogen-free (SPF) animal facility of Tianjin University of Traditional Chinese Medicine, under controlled temperature 22 ± 2 °C and humidity 40 ± 5% with a 12-h light/dark cycle, and received standard diet and water. Three different sets of animals were used: (1) SD male rats were used for in vivo pharmacokinetic study; (2) C57/BL mice were used for in vivo assessment of the cardioprotective effect of Rg3-loaded Pluronic F127 micelles at a toxic DOX dosing. (3) Balb/C mice were used for in vivo assessment of tumor weight. The animals were fasted for 12 h before the experiment, but allowed free access to water. The experimental procedures conformed to directive 2010/63/EU of the European Parliament and all animals were handled in accordance with the guidelines of Tianjin University of Traditional Chinese Medicine Animal Research Committee (TCM-LAEC2014005).
4
Intracranial Xenograft Mouse Model for Glioblastoma
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The intracranial xenograft mouse model was generated according to our previous report [33 (link)]. Six-week-old female C57/BL mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and bred in rooms under controlled conditions of temperature, humidity, photoperiods, and air exchange. GL261-Luc cells were cultured for up to three passages and maintained in logarithmic growth phase. Then, the mice were anesthetized (1% pentobarbital, 0.4 mL/100 g mouse body weight, i.p.) and approximately 3 × 105 GL261-Luc cells, resuspended in 5 μL of PBS, were injected stereotactically into the left striatum. Animals were euthanized when they demonstrated signs of morbidity including hunched posture, lethargy, difficulty moving, and weight loss.
5
B16F10 Murine Melanoma Xenograft Model
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B16F10 murine melanoma cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Male C57BL mice at 5–6 weeks of age (approximately 20 g in weight) were purchased from the Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). All of the animals were treated with care according to the guidelines outlined in the Guide for the Care and Use of Laboratory Animals. All procedures were implemented after approval from the Animal Care and Use Committee of Jilin University.
6
Animal Housing and Handling Protocol
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ICR mice (male, 18–22 g), Sprague–Dawley (SD) rats (male, 180–200 g), and C57BL mice (male, 22 ± 3 g) were supplied by Vital River Laboratory Animal Technology Co., Ltd (Beijing, China), and they were housed in a controlled environment (21 ± 2 °C, 12-h light/dark cycle) with free access to food and water during the acclimatization and study periods in an SPF-grade room. The research was conducted in accordance with the institutional guidelines and ethics, and approved by the Laboratories Institutional Animal Care and Use Committee of the Chinese Academy of Medical Sciences and Peking Union Medical College. The research was conducted in accordance with all guidelines and ethics of the Chinese Council on Animal Care.
7
Tumor Growth Kinetics in C57/BL Mice
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Six-week-old male C57/BL mice (Vital River, Beijing, China) received subcutaneous injections of 5 × 105 Hepa1–6 cells. Tumor volumes were measured every 2 days. The following formula was used to calculate the tumor volume: volume = (length × width2)/2.
8
Isolation and Characterization of Stem Cells
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Umbilical cord tissues were obtained from healthy pregnant women admitted to our hospital for delivery. These women signed a written informed consent. C57BL mice (6-8 weeks) were ordered from Beijing Vital River Laboratory Animal Technology Co. (China). Consumables such as F12 culture medium and pipettes were ordered from Sigma (St. Louis, USA). CUR, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), and MPP+ were ordered from Sigma (St. Louis, USA). Fetal calf serum was from HyClone (Waltham, USA). All ELISA kits were from IBL (Germany). All antibodies were purchased from Abcam (UK), BD Biosciences (San Jose, CA), BD (Franklin Lakes, NJ), and BioLegend (Cambridge, UK). Chromogenic kits were ordered from Shanghai to Ruicheng Biotechnology Co. Ltd (Shanghai, China). The SH-SY5Y cell line was obtained from the Shanghai Institute of Cell Biology of the Chinese Academy of Sciences.
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