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Gm00038

Manufactured by Coriell Institute for Medical Research
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GM00038 is a human female skin fibroblast cell line obtained from Coriell Institute for Medical Research. It is a primary cell line that can be used for various cell-based research applications.

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Lab products found in correlation

Hek293 cell, by American Type Culture Collection (2 mentions) Ag11513, by Coriell Institute for Medical Research (2 mentions) Ag11498, by Coriell Institute for Medical Research (2 mentions) Ag03198, by Coriell Institute for Medical Research (2 mentions) Cytotune ips 2.0 sendai reprogramming kit, by Thermo Fisher Scientific (2 mentions) Gm08680, by Coriell Institute for Medical Research (2 mentions) Gm05756, by Coriell Institute for Medical Research (2 mentions) Ag05229, by Coriell Institute for Medical Research (1 mentions) Ag03141, by Coriell Institute for Medical Research (1 mentions) Penicillin streptomycin, by Corning (1 mentions) L glutamine, by Corning (1 mentions) Sw1353, by American Type Culture Collection (1 mentions) Gm09503, by Coriell Institute for Medical Research (1 mentions)

5 protocols using gm00038

1

Cell Culture of HGPS and Control Fibroblasts

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Human fibroblast cells from HGPS patients (AG03198, 10-year-old female; AG03199, 10-year-old female; AG11513, 8-year-old female; AG11498, 14-year-old male), and normal person (GM00038, 9-year-old female N9) were obtained from Coriell Cell Repositories (Camden, NJ, USA) and maintained in Eagle’s minimal essential medium supplemented with 15% fetal bovine serum, and 2 mM glutamine without antibiotics. HEK293 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in liquid medium containing 10% FBS, and 1% penicillin–streptomycin at 37 °C with 5% CO2.
Kang S.M., Yoon M.H., Ahn J., Kim J.E., Kim S.Y., Kang S.Y., Joo J., Park S., Cho J.H., Woo T.G., Oh A.Y., Chung K.J., An S.Y., Hwang T.S., Lee S.Y., Kim J.S., Ha N.C., Song G.Y, & Park B.J. (2021). Progerinin, an optimized progerin-lamin A binding inhibitor, ameliorates premature senescence phenotypes of Hutchinson-Gilford progeria syndrome. Communications Biology, 4, 5.
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2

Fibroblast Cell Lines for Aging Research

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Primary human fibroblast cells from HGPS patients (AG03198, 10-year-old female, HGPS1; AG11513, 8-year-old female, HGPS2; AG11498, 14-year-old male, HGPS3), WRN patients (AG06300, 37-year-old male, WRN1; AG05229, 25-year-old male, WRN2; AG03141, 30-year-old female, WRN3) and unaffected controls (GM00038, 9-year-old female, N9; AG09603, 81-year-old female, N81) were obtained from Coriell Cell Repositories (Camden, NJ, USA) and maintained in Eagle’s Minimal Essential Medium (EMEM) supplemented with 15% fetal bovine serum (FBS) and 2 mM glutamine or in EMEM with 26 mM HEPES without antibiotics. HEK293 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in liquid medium (DMEM) containing 10% FBS and 1% penicillin–streptomycin at 37 °C with 5% CO2. All cell lines were established in our laboratory under study protocols approved by the PNU IRB, in accordance with relevant guidelines and regulations.
Kang S.M., Yoon M.H., Lee S.J., Ahn J., Yi S.A., Nam K.H., Park S., Woo T.G., Cho J.H., Lee J., Ha N.C, & Park B.J. (2021). Human WRN is an intrinsic inhibitor of progerin, abnormal splicing product of lamin A. Scientific Reports, 11, 9122.
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3

Fibroblast Reprogramming to iPSCs

Cited 1 time
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Human fibroblasts from three apparently healthy individuals (GM00038, GM08680, GM05756) were purchased from Coriell. Reprogramming was performed using the CytoTune-iPS 2.0 Sendai reprogramming kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Briefly, fibroblasts were plated at 20,000 cells/cm2 (link) and cultured until they were 30–60% confluent. Cells were transduced with the Sendai reprogramming vectors at an MOI of 5:5:3 (KOS:hc-Myc:hKlf4). Cells were maintained in fibroblast media until passaged onto VN-coated plates on day 7. On day 8, medium was changed to E8 Medium and cells were cultured for additional 20 days. On day 28, colonies were picked using a 25-gauge needle to cut single colonies into 6–8 pieces and transferred onto VN-coated plates containing E8 Medium only or supplemented with Y-27632 or CEPT for 48 h. After 48 h, cells were kept in E8 Medium only. Confluency was assessed 8 days after colony picking using the Celigo Imaging Cytometer (Nexcelom Biosciences).
Chen Y., Tristan C.A., Chen L., Jovanovic V.M., Malley C., Chu P.H., Ryu S., Deng T., Ormanoglu P., Tao D., Fang Y., Slamecka J., Hong H., LeClair C.A., Michael S., Austin C.P., Simeonov A, & Singeç I. (2021). A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells. Nature methods, 18(5), 528-541.
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4

Fibroblast Culturing Protocol for Research

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Primary fibroblasts derived from healthy controls (GM00038 and GM09503) were obtained from Coriell Institute for Medical Research (Camden, NJ). Each SWS-derived fibroblast line was obtained by the referring clinician and grown via a clinical lab service and then sent to us with consent through an approved IRB (Ferreira et al., 2018 (link)). SW1353 was obtained from ATCC.
Fibroblasts and SW1353 were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) containing 1 g/L glucose supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1X Penicillin-Streptomycin (Corning) and 200 mm L-glutamine (Corning). HEK293T cells were cultured in 4.5 g/L glucose DMEM supplemented with the same components as mentioned above.
Xia Z.J., Mahajan S., Paul Daniel E.J., Ng B.G., Saraswat M., Campos A.R., Murad R., He M, & Freeze H.H. (2022). COG4 mutation in Saul-Wilson syndrome selectively affects secretion of proteins involved in chondrogenesis in chondrocyte-like cells. Frontiers in Cell and Developmental Biology, 10, 979096.
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5

Fibroblast Reprogramming to iPSCs

Cited 1 time
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Human fibroblasts from three apparently healthy individuals (GM00038, GM08680, GM05756) were purchased from Coriell. Reprogramming was performed using the CytoTune-iPS 2.0 Sendai reprogramming kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Briefly, fibroblasts were plated at 20,000 cells/cm2 (link) and cultured until they were 30–60% confluent. Cells were transduced with the Sendai reprogramming vectors at an MOI of 5:5:3 (KOS:hc-Myc:hKlf4). Cells were maintained in fibroblast media until passaged onto VN-coated plates on day 7. On day 8, medium was changed to E8 Medium and cells were cultured for additional 20 days. On day 28, colonies were picked using a 25-gauge needle to cut single colonies into 6–8 pieces and transferred onto VN-coated plates containing E8 Medium only or supplemented with Y-27632 or CEPT for 48 h. After 48 h, cells were kept in E8 Medium only. Confluency was assessed 8 days after colony picking using the Celigo Imaging Cytometer (Nexcelom Biosciences).
Chen Y., Tristan C.A., Chen L., Jovanovic V.M., Malley C., Chu P.H., Ryu S., Deng T., Ormanoglu P., Tao D., Fang Y., Slamecka J., Hong H., LeClair C.A., Michael S., Austin C.P., Simeonov A, & Singeç I. (2021). A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells. Nature methods, 18(5), 528-541.
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