Enhanced chemiluminescence ecl reagent

Primary antibodies against PPAR-γ (cat. no. sc-7272), C/EBP-α (cat. no. sc-61), phosphorylated (p)-STAT3 (cat. no. sc-8059) and total (T)-STAT3 (cat. no. sc-8019) were purchased from Santa Cruz Biotechnology, Inc. The primary antibody against FAS (cat. no. 9452) was obtained from BD Biosciences. Primary antibodies against p-HSL (S563) (cat. no. 4139) and p-HSL (S565) (cat. no. 4137) were acquired from Cell Signaling Technology, Inc. Primary antibodies against COX-2 (cat. no. 160106) and T-HSL (cat. no. 10006371) were purchased from Cayman Chemical Company. Primary antibody against perilipin A (cat. no. 3948-200) was purchased from BioVision, Inc. The primary antibody of β-actin (A5441), iso-butylmethylxanthine (IBMX), dexamethasone, insulin, Oil Red O solution, Isoproterenol, and Free Glycerol Reagent were obtained from MilliporeSigma. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were purchased from Welgene, Inc. Fetal calf serum (FCS) and trypan blue dye were purchased from Gibco; Thermo Fisher Scientific, Inc. Enhanced chemiluminescence (ECL) reagents were purchased from Advansta Inc.

Regents were obtained as follows: LPS (Escherichia coli 055: B5, L-2880), d-GalN (G0500), STAT3 inhibitor Stattic (S7947) were from Sigma-Aldrich (St. Louis, MO, USA). Abs against cleaved caspase-3, cleaved poly (ADP-ribose) polymerase (PARP), the HRP-conjugated anti-rabbit IgG, and the HRP-conjugated anti-mouse IgG were from Cell Signaling Technology (Ann Arbor, MI, USA). Ab against β-actin was purchased from 4A BIOTECH (Beijing, China). Mouse TNF-α ELISA Kit (EMC 102a) and mouse IL-6 ELISA Kit (EMC004) were from NeoBioscience Technology Company (Shenzhen, China). The test kits for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The Radio Immunoprecipitation Assay (RIPA) Lysis Buffer (P0013B), PMSF (ST506), the caspase-3 Activity Assay Kit (C1116), the caspase-8 Activity Assay Kit (C1152), and the caspase-9 Activity Assay Kit (C1158) were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). The BCA protein assay kit was purchased from Pierce Biotechnology (Rockford, IL, USA). The enhanced chemiluminescence (ECL) reagent was obtained from Advansta (Menlo Park, CA, USA).