Mouse anti cd3 and anti cd28 antibodies

Piperine, TMA, dexamethasone, and OVA (Grade VI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Inject Alum was obtained from Pierce Biotechnology (Rockford, IL, USA). The T-PER tissue protein extraction reagent was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Recombinant mouse IL-4 and human IL-4 were purchased from BD Biosciences (San Diego, CA, USA). The mouse IL-4 ELISA kit was purchased from BD Biosciences (San Diego, CA, USA). The MagniSort^TM mouse CD4⁺ T cell enrichment kit was obtained from Invitrogen (Carlsbad, CA, USA). Antibodies specific for STAT6, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-rabbit, anti-mouse antibodies were purchased from Santa Cruz (CA, USA). Antibodies for mouse phospho-STAT6 and CCR3 were obtained from Abcam (Cambridge, UK) and human phospho-STAT6 was purchased from Cell Signaling (Danvers, MA, USA). Mouse anti-CD3 and anti-CD28 antibodies were purchased from BioLegend (San Diego, CA, USA). The RNeasy^® Mini Kit was purchased from Qiagen (Valencia, CA, USA).

Imject^TM Alum Adjuvant was obtained from Pierce Biotechnology (Rockford, IL, USA). MitoSOX^TM Red was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Mouse IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and IgE ELISA kits were purchased from BD Biosciences (San Diego, CA, USA). IL-13 ELISA kit was obtained from R&D Systems (Minneapolis, MN, USA). Specific primary antibodies for signal transducer and activator of transcription 6 (STAT6), p21, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Primary antibody for phospho-STAT6 was obtained from Abcam (Cambridge, UK). Antibodies for phospho-STAT1, STAT1, phospho-STAT6, STAT6, phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-JNK, JNK, NRF2, and HO-1 were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-CD3 and anti-CD28 antibodies were purchased from Biolegend (San Diego, CA, USA). SP600125 was purchased from Calbiochem (San Diego, CA, USA). U0126 was obtained from Promega (Madison, WI, USA) and 2′,7′-Dichlorofluorescein-diacetate (DCFH-DA) was purchased from Invitrogen (Carlsbad, CA, USA). Propidium iodide (PI), SB203580, TMA, ovalbumin (OVA) (Grade VI), dexamethasone (Dex), concanavalin A (Con A), N-acetylcysteine (NAC), and MQL were purchased from Sigma-Aldrich (St. Louis, MO, USA).