Four-arm poly(ethylene glycol) (4-arm PEG) (Mn = 40,000) and linear PEG (Mn = 6000) were provided by NOF Co., Ltd. (Tokyo, Japan) and purified by precipitations in hexane. 2-Hydroxy-4′-(2-hydroxyehoxy)-2-methylpropiophenone (irgacure 2959) and linear PEG (Mn = 3350 and 10,000) were purchased from Sigma-Aldrich Co., LLC. (St. Louis, MO, USA). Tetrahydrofuran (THF) ultradehydrated, diisopropylamine, titanium(III) chloride solution (20%), iron(III) chloride hexahydrate, and phosphoryl chloride were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan) and used as received. Acryloyl chloride was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and used as received. Vanadium(III) chloride hexahydrate was purchased from Thermo Fisher Scientific Chemicals Inc. (Waltham, MA, USA) and used as received.
Iron 3 chloride hexahydrate
Manufactured by Fujifilm
Sourced in Japan
Iron (III) chloride hexahydrate is a chemical compound commonly used in laboratory settings. It is an inorganic salt with the chemical formula FeCl3·6H2O. The compound appears as reddish-brown crystals or a crystalline solid. It is soluble in water and other polar solvents.
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Lab products found in correlation
Sodium hydroxide (naoh), by Fujifilm (3 mentions)
Acryloyl chloride, by Tokyo Chemical Industry (1 mentions)
Phosphoryl chloride, by Fujifilm (1 mentions)
Linear peg, by Merck Group (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
Sodium dihydrogen phosphate dihydrate, by Fujifilm (1 mentions)
Calcium dichloride, by Fujifilm (1 mentions)
Magnesium dichloride, by Fujifilm (1 mentions)
Zinc chloride, by Fujifilm (1 mentions)
Decane, by Fujifilm (1 mentions)
Ofloxacin, by Fujifilm (1 mentions)
1 octanol, by Fujifilm (1 mentions)
2 morpholinoethanesulfonic acid, by Dojindo Laboratories (1 mentions)
Norfloxacin, by Fujifilm (1 mentions)
8 m naoh, by Fujifilm (1 mentions)
Anti β actin monoclonal antibody, by Fujifilm (1 mentions)
Mitotracker, by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Celltracker green cmfda, by Thermo Fisher Scientific (1 mentions)
7 protocols using iron 3 chloride hexahydrate
1
Synthesis and Characterization of Multifunctional PEG Hydrogels
2
Synthesis of Iron-Based Nanomaterials
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Iron (III) chloride hexahydrate (FeCl3·6H2O), fumaric acid (C4H4O4) and sodium hydroxide (NaOH) were purchased from Fujifilm Wako, Japan. Ovalbumin (OVA) was purchased from Sigma-Aldrich.
3
Characterization of Fluoroquinolone Derivatives
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Norfloxacin (NFLX), ofloxacin (OFLX), tosufloxacin tosylate monohydrate (TFLX), calcium dichloride, magnesium dichloride, iron(II) dichloride tetrahydrate, iron(III) chloride hexahydrate, aluminum(III) chloride hexahydrate, zinc chloride, lanthanum chloride heptahydrate, 1-octanol, sodium dihydrogen phosphate dihydrate, sodium chloride, sodium hydroxide, decane, 8 M NaOH, and 0.1 vol% trifluoroacetic acid-acetonitrile were purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). Ciprofloxacin hydrochloride monohydrate (CPFX), levofloxacin (LVFX), enoxacin sesquihydrate (ENX), fleroxacin (FLRX), and prulifloxacin (PUFX) were purchased from TCI (Tokyo, Japan). Gatifloxacin (GFLX) and sparfloxacin (SPFX) were purchased from Funakoshi Co., Ltd (Tokyo, Japan). Moxifloxacin (MFLX) was purchased from Combi-Blocks Inc. (San Diego, USA). 2-Morpholinoethanesulfonic acid (MES) was purchased from Dojindo laboratories (Tokyo, Japan). 0.1 vol% trifluoroacetic acid-distilled water was purchased from Kanto chemical co., inc (Tokyo, Japan). Soybean lecithin was provided by Tsuji Oil Mills co., Ltd (Mie, Japan) (SLP-White, phosphatidylcholine (24-32 %), phosphatidylethanolamine (20-28 %), phosphatidylinositol (12-20 %), phosphatidic acid (8-15 %), and lysophosphatidylcholines (1-5 %) (based on the product information provided by the manufacturer)).
4
Synthesis and Characterization of HMRhoNox-M
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HMRhoNox-M was prepared according to the reported procedure [24] (link). We purchased iron (III) chloride hexahydrate (Wako, Osaka, Japan), LysoTracker (Molecular Probes, Eugene, OR), MitoTracker (Molecular Probes), CellTracker Green CMFDA (Molecular Probes), Hoechst 33342 (Molecular Probes), Cellmatrix Type I-P collagen (Nitta Gelatin, Inc., Osaka, Japan), anti-human transferrin receptor antibody (Zymed, 13-6800; San Francisco, CA), anti-ferroportin 1 antibody (Novus Biologicals, NBP1-21502; Littleton, CO), anti-CD10 monoclonal antibody (Novocastra, NCL-L-CD10-270; Newcastle, UK), anti β-actin monoclonal antibody (Wako, 011-24554; Tokyo, Japan), Stealth siRNAs for human transferrin receptor 1 (TfR1) (HSS110674, HSS110676 and HSS186305) and a non-targeting siRNA (HSS12935-112) as a negative control (Invitrogen, Carlsbad, CA). We prepared ferric nitrilotriacetate (Fe(III)-NTA) solution by mixing 300 mM ferric nitrate and 600 mM nitrilotriacetate, followed by pH adjustment to 7.4 with sodium hydrogen bicarbonate as described [28] (link). The other chemicals were of analytical grade.
5
Synthesis of Iron Oxide Nanoparticles
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All materials (analytic grade) were used as received without further purification.
Iron(iii ) chloride hexahydrate (FeCl3·6H2O), ethanol, sodium acetate, hexylamine, acetonitrile, methanol, N,N-dimethylacetamide (DMAc), benzene, toluene, THF, and [Emim+][NTf2−] were purchased from Wako Pure Chemicals.
N-[3-(Trimethoxysilyl)propyl]aniline, methyl methacrylate (MMA) 1,8-bis(dimethylamino)naphthalene, trioctylamine (TOA), copper(i ) bromide, 2,2′-bipyridine, and trimethylamine were purchased from Sigma-Aldrich Co. LLC.
Iron(
N-[3-(Trimethoxysilyl)propyl]aniline, methyl methacrylate (MMA) 1,8-bis(dimethylamino)naphthalene, trioctylamine (TOA), copper(
6
Synthesis of α-NixFe1-xOOH Nanoparticles
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For preparing the α-NixFe1−xOOH NPs, iron (III) chloride hexahydrate (Wako, Japan, 095-00875) and nickel (II) chloride (Wako, Japan, 141-01062) were dissolved in 30 mL of pure water under magnetic stirring. The concentration and molar ratio of nickel are presented in Table 9 . Once the mixture solution was thoroughly homogenized, 3 mol L−1 sodium hydroxide (Wako, Japan, 194-02135) aqueous solution was added until the pH reached 13.
The mixture solution underwent ultrasonication for 20 min after vigorous stirring. Furthermore, the mixture solution was stirred for an additional 20 min. The obtained mixture was then transferred into a Teflon-lined stainless-steel autoclave and maintained at 80 °C for a hydrothermal reaction period of 24 h. After naturally cooling to RT, the solid products were washed with pure water and ethanol to remove the neutral electrolyte.
The solid products were dried at 60 °C for 12 h and allowed to cool naturally. These samples were, respectively, abbreviated as Ni0, Ni5, Ni10, Ni15, Ni20, Ni30, Ni40, and Ni50.
The mixture solution underwent ultrasonication for 20 min after vigorous stirring. Furthermore, the mixture solution was stirred for an additional 20 min. The obtained mixture was then transferred into a Teflon-lined stainless-steel autoclave and maintained at 80 °C for a hydrothermal reaction period of 24 h. After naturally cooling to RT, the solid products were washed with pure water and ethanol to remove the neutral electrolyte.
The solid products were dried at 60 °C for 12 h and allowed to cool naturally. These samples were, respectively, abbreviated as Ni0, Ni5, Ni10, Ni15, Ni20, Ni30, Ni40, and Ni50.
7
Removal of Iron (III) Stains on Cotton
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The model stain was prepared using cotton canequim (Laundry Science Association in Japan) as a model fabric sample, and iron (III) chloride hexahydrate (Fujifilm Wako Chemicals, Assay＞99％) as the material. The components of cleaning liquid were oxalic acid (Fujifilm Wako Chemicals., Assay＞98％) , hydrochloric acid (Fujifilm Wako Chemicals., Assay 35-37％) , and citric acid (Fujifilm Wako Chemicals) as acidic agents; and sodium hydrosulfite (Fujifilm Wako Chemicals) , thiourea dioxide (Fujifilm Wako Chemicals) and L (＋) -ascorbic acid (Fujifilm Wako Chemicals., Assay＞99.6％) as reducing agents. Ethylenediaminetetraacetic acid disodium salt (EDTA, Fujifilm Wako Chemicals) , diethylenetriaminepentaacetic acid (DTPA, Tokyo Chemical Industry, Assay＞98％) , nitrilotriacetic acid (NTA, Fujifilm Wako Chemicals) , tetrasodium 3-hydroxy- were used for the pH adjustment of the cleaning liquid.
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