1H and 13C NMR spectra were recorded on a JEOL ECZ500 (500 MHz for 1H NMR) or a JEOL ECZ400 (400 MHz for 1H NMR) spectrometers. Chemical shifts are denoted in δ (ppm) relative to residual solvent peaks as internal standard (DMSO-d6, 1H δ 2.50, 13C δ 39.5). ESI-MS spectra were measured by a Thermo Scientific Exactive mass spectrometer or a SHIMADZU LCMS-2050 spectrometer. Optical rotations were recorded on a JASCO P-1030 polarimeter. High-performance liquid chromatography (HPLC) experiments were performed with a SHIMADZU HPLC system equipped with a LC-20AD intelligent pump. MS/MS analysis was performed with amaZon SL-NPC (Bruker Daltonics) using helium gas with an amplitude value 1.0 V. The chemical structures of enzyme substrates and enzymatic reaction products are analyzed by MS/MS (Supplementary data 1 ) and/or NMR (Supplementary data 2 ). All reagents were used as supplied unless otherwise noted.
Exactive mass spectrometer
Manufactured by Shimadzu
The Exactive mass spectrometer is an analytical instrument designed for accurate mass measurement. It utilizes Orbitrap technology to provide high-resolution and high-mass accuracy for the detection and analysis of a wide range of chemical compounds.
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Lab products found in correlation
P 1030 polarimeter, by Jasco (2 mentions)
Hplc system, by Shimadzu (2 mentions)
Amazon sl npc, by Bruker (1 mentions)
Ecz 500, by JEOL (1 mentions)
Ecz 400, by JEOL (1 mentions)
Silica gel 60n, by Kanto Chemical (1 mentions)
Eca 500 spectrometer, by JEOL (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Lcms 2020 spectrometer, by Shimadzu (1 mentions)
2 protocols using exactive mass spectrometer
1
Spectroscopic Characterization of Compounds
2
General Characterization Methods for Organic Compounds
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General Methods 1 H-and 13 (link) C-NMR spectra were recorded on a JEOL ECA 500 spectrometer (500 MHz for 1 H-NMR). Chemical shifts are denoted in δ (ppm) relative to residual solvent peaks as internal standards (CDCl 3 , 1 H δ 7.26, 13 C δ 77.0). Electrospray ionization (ESI)-MS spectra were recorded on a Thermo Scientific Exactive mass spectrometer or a SHIMADZU LCMS-2020 spectrometer. Specific rotations were recorded on a JASCO P-1030 polarimeter. HPLC experiments were performed with a SHIMADZU HPLC system equipped with an LC-20AD intelligent pump. All reagents were used as supplied unless otherwise stated. Analytical TLC was performed using E. Merck Silica gel 60 F 254 precoated plates. Column chromatography was performed using 40-50 µm Silica Gel 60N (Kanto Chemical Co., Inc., Japan).
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