To assess effect of short-term drug pretreatment on 2D/3D growth, cells are plated in six-well plates and allowed to recover for 24 h. At this point, drug-containing media was added to each well. After 24 h of treatment, cells were then were plated for mammospheres or 2D proliferation assays. 2D proliferation was determined after 3 days by viable cell counts, and assessed as a percentage of the DMSO control. All treatments were carried out in triplicate, and repeated a minimum of three times. The error bars represent the standard error of the mean of the three replicates. The small molecule drugs that are used to treat the cells for various experiments are: TSA (Selleck) Doxirubicin (Selleck), Paclitaxel (Selleck), 5-Fluorouracil (Sigma), SAHA (Selleck), GSK126 (Cayman Chem), MS275 (Selleck), MGCD0103 (Selleck), MC1568 (Selleck), MC1575 (Gift of Dr. Antonello Mai), Apicidin (Sigma), Droxinostat (Selleck). All drugs are prepared in DMSO, which was used as vehicle control in each assay.
Mc1575
Manufactured by Merck Group
Sourced in United States
The MC1575 is a laboratory centrifuge designed for general-purpose sample separation and preparation. It features a compact, benchtop design and can accommodate sample volumes up to 1.5 liters. The centrifuge is capable of reaching a maximum speed of 4,000 rpm, enabling efficient separation of materials with varying densities.
Automatically generated - may contain errors
Lab products found in correlation
Apicidin, by Merck Group (2 mentions)
Paclitaxel, by Selleck Chemicals (2 mentions)
Mgcd0103, by Selleck Chemicals (2 mentions)
Ms 275, by Selleck Chemicals (2 mentions)
Doxirubicin, by Selleck Chemicals (2 mentions)
Mc1568, by Selleck Chemicals (2 mentions)
Droxinostat, by Selleck Chemicals (2 mentions)
Gsk126, by Cayman Chemical (2 mentions)
5 fluorouracil, by Merck Group (2 mentions)
2 protocols using mc1575
1
Evaluating Short-Term Drug Effects on Cell Growth
2
Evaluating Drug Effects on 2D/3D Cell Growth
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To assess effect of short-term drug pretreatment on 2D/3D growth, cells are plated in six-well plates and allowed to recover for 24 h. At this point, drug-containing media was added to each well. After 24 h of treatment, cells were then were plated for mammospheres or 2D proliferation assays. 2D proliferation was determined after 3 days by viable cell counts, and assessed as a percentage of the dimethyl sulfoxide control. All treatments were carried out in triplicate, and repeated a minimum of three times. The error bars represent the standard error of the mean of the three replicates. The small molecule drugs that are used to treat the cells for various experiments are: TSA (Selleck, Boston, MA, USA) Doxirubicin (Selleck), Paclitaxel (Selleck), 5-Fluorouracil (Sigma), SAHA (Selleck), GSK126 (Cayman Chem), MS275 (Selleck), MGCD0103 (Selleck), MC1568 (Selleck), MC1575 (Gift of Dr. Antonello Mai), Apicidin (Sigma), Droxinostat (Selleck). All drugs are prepared in dimethyl sulfoxide, which was used as vehicle control in each assay.
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