Phosphor 1 κbα ser32

Manufactured by Cell Signaling Technology

Sourced in United States

Phosphor-I-κBα (Ser32) is a lab equipment product that detects the phosphorylation of I-κBα at serine 32. I-κBα is a key regulator of the NF-κB signaling pathway, and phosphorylation of I-κBα at serine 32 is a critical step in the activation of NF-κB.

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Lab products found in correlation

3 protocols using phosphor 1 κbα ser32

Immunoblot Analysis of NF-κB Pathway

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Phospho-IKKα/β (Ser176/180), I-κBα, phosphor-I-κBα (Ser32) and anti-α-tubulin (3873p) were purchased from Cell Signaling Technology (Beverly, MA). Anti-TRAF6 (H-274), anti-p65 (C-20), anti-ub (P4D1) and Sp1 (sc-59-G) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-CHIP antibody was developed in our lab (21 (link)). MG132 was purchased from Millipore (Billerica, MA). Cyclohexanone (CHX) was purchased from Amresco (AMRESCO Inc., OH).

Li S., Shu B., Zhang Y., Li J., Guo J., Wang Y., Ren F., Xiao G., Chang Z, & Chen D. (2014). CHIP Regulates Osteoclast Formation through Promoting TRAF6 Protein Degradation. Arthritis & rheumatology (Hoboken, N.J.), 66(7), 1854-1863.

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Regulation of NF-κB Signaling by CHIP

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pCMV/Myc-TRAF6, TRAF2, and TRAF5 was provided by Dr. Lingqiang Zhang (State Key Laboratory of Proteomics, Beijing, China). pEFNeo/HA-CHIP, pRKIM/Myc-CHIP, and pRKIM/Myc-CHIP (H260Q) were generated in our lab as described previously.^{23 (link)} I-κBα, phosphor-I-κBα (Ser32) and anti-α-tubulin (3873p) were purchased from Cell Signaling Technology (Beverly, MA). Anti-TRAF6 (H-274) and anti-p65 (C-20) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-CHIP antibody was developed in our lab.^{16 (link)} MG132 was purchased from Millipore (Billerica, MA). Cyclohexanone (CHX) was purchased from Amresco (AMRESCO Inc., OH).

Wang T., Li S., Yi D., Zhou G.Q., Chang Z., Ma P.X., Xiao G, & Chen D. (2018). CHIP regulates bone mass by targeting multiple TRAF family members in bone marrow stromal cells. Bone Research, 6, 10.

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Protein signaling pathway analysis

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Primary antibodies: Akt (#9272), phospho-Akt (Thr308) (#4056), FoxO3a (#2497), phospho-FoxO3a (Ser253) (#9466), AMPKα (#2603), phospho-AMPKα (Thr172) (#2535), IκBα (#4814), phosphor-IκBα (Ser32) (#2859), K48-linkage polyubiquitin (#4289), NF-κB p65 (#4764), phospho-NF-κB p65 (Ser563) (#3033), and SIRT1 (#3931), were purchased from Cell Signaling (Danvers, MA, United States). PGC1α (ab54481) was purchased from Abcam (Cambridge, MA, United States). MuRF-1 (sc-398608) and GAPDH (sc-47724) were purchased from Santa Cruz Biotechnology (Dallas, TX, United States). Goat anti-rabbit (#7074) and horse anti-mouse (#7076) HRP conjugated secondary antibodies were purchased from Cell signaling (Danvers, MA, United States).

Liu H.W, & Chang S.J. (2018). Moderate Exercise Suppresses NF-κB Signaling and Activates the SIRT1-AMPK-PGC1α Axis to Attenuate Muscle Loss in Diabetic db/db Mice. Frontiers in Physiology, 9, 636.

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