The Raji cells were plated at 2×105 cells per well in a 6-well plate. After 24–48 h, the Raji cells were treated with or without emodin at concentrations of 6.25, 12.5, 25 and 50 µM for 24 h. At the end of the exposure, the Raji cells were collected and washed twice in PBS, an then suspended in 0.5 ml binding buffer containing 5 µl annexin V-FITC and 10 µl PI, and incubated in the dark at room temperature for 30 min. The samples were then analyzed using a FACScan flow cytometer (BD Biosciences, Hercules, CA, USA), and 1×104 events were collected, recorded on a dot plot, and analyzed using ModFit software version 2.0 (Verity Software House, Inc., Topsham, ME, USA).
Modfit software version 2
Manufactured by Verity Software House
Sourced in United States
ModFit software version 2.0 is a data analysis software designed for the flow cytometry industry. The software's core function is to analyze and model cell population data generated from flow cytometry experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using modfit software version 2
1
Emodin Induces Apoptosis in Raji Cells
2
Cell Cycle Analysis of NSCLC Cells
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Propidium iodide (PI) staining followed by flow cytometric analysis was performed to determine the effects of tnv-1 on the cell cycle of the treated cells. NSCLC cells were plated at 50,000–100,000/well in a 6-well plate. The next day tnv-1 (10 μM) was added. After 72 h, cells were fixed overnight in cold 70% ethanol, incubated with 0.2 mg/mL RNase A (Sigma-Aldrich) for one hour at 37°C and stained with PI (10 μg/mL, Sigma-Aldrich). The PI fluorescence was collected from 20,000 events in FL3-H channel (linear scale) on FACSCalibur Flow Cytometer (Becton Dickinson). The obtained results were analyzed using ModFit software version 2.0 (Verity Software House) and FlowJo software v. 7.2.5 (Tree Star, Inc.).
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