C3h hej

All animal experiments were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals (U.S. Department of Health and Human Services, Public Health Services, National Institute of Health, NIH publication no. 86–23, 1985)34 , and approved by the Ethical Committee for Animal Experiments at the University of Tokyo. EGFP-overexpressing C57BL/6 mice (C57BL/6-Tg (CAG-EGFP)) were purchased from Japan SLC Inc. C3H/HeN and C3H/HeJ mice were purchased from CLEA Japan. All mice were allowed access to feed and water ad libitum.

C57BL/6J, C3H/HeJ and C3H/HeN mice were purchased from Clea Japan (Tokyo, Japan). The genetic background of the genetically engineered mice used was C57BL/6J. Il6^−/− (IL-6-KO), Cd19-Cre, Jh^−/− (Jh-KO) and C57BL/6-Ly5.1 mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA); mice with homozygous Cd19 deficiency (Cd19^cre/cre) were used as Cd19^−/− mice. Myd88^−/− (Myd88-KO), Ticam^−/− (Trif-KO) and Tlr4^−/− (TLR4-KO) mice were purchased from Oriental BioService (Kyoto, Japan). Fcα/μR^−/− (Fcα/μR-KO) mice were generated in our laboratory, as previously described, and backcrossed onto the C57BL/6 genetic background for 12 generations. Only female mice between the ages of 8 weeks and 12 weeks were used for the experiments. All experiments were performed in accordance with the guidelines of the animal ethics committee of the University of Tsukuba Animal Research Center.

All animal experiments were approved by the Animal Research Committee of the RIKEN BioResource Center and were performed in accordance with RIKEN guidelines for animal experiments. Three laboratory mouse strains, C57BL/6J, C3H/HeJ, and DBA/2J, were purchased from a commercial breeder (CLEA Japan, Inc., Japan) and maintained in our facility (RIKEN BioResource Center Research Building for Animal Models of Human Disease). All mice were maintained at constant room temperature (23 °C ± 2 °C) and humidity (55 ± 10%) under a light/dark cycle of 12/12 h in a specific pathogen-free (SPF) environment. The pathogens were specified in categories A and B of the International Council for Laboratory Animal Science. Mice were weaned between the ages of 4 and 5 weeks. Mice had ad libitum access to water and conventional chow diet, CA-1 (CLEA Japan, Inc., Japan).