Cell extracts were prepared using RIPA buffer (20mM Tris, pH 7.4, 137 mM NaCl, 1% NP-40, 0.25% Deoxycholate, 0.1% SDS, 1mM EDTA and 1% protease inhibitor cocktail). Protein concentration was determined by BCA kit (Pierce; Rockford, IL) and 100 µg protein was analyzed by Western blotting as described previously69 (link). Anti-p53 (sc-98, Santa Cruz) was used at 1:1000 (2 µg/mL) dilution and anti-Bcl-2 (sc-492, Santa Cruz) and anti-β-actin (Sigma) were used at 1:1000 dilutions. SPDEF antibodies (gift from Dr. Jeffery Whitsett, Children’s Hospital, University of Cincinnati, OH) were used at 1:5000 dilution. Proteins were detected using ECL and visualized by chemiluminescence (Perkin Elmer, Waltham, MA) using the FujiFilm Image Reader LAS-4000 (Valhalla, NY). The uncropped scans of the most important Western blots are included as a supplementary figure in the Supplementary Information.
Anti p53 sc 98
Manufactured by Santa Cruz Biotechnology
Anti-p53 (sc-98) is a lab equipment product from Santa Cruz Biotechnology. It is an antibody that targets the p53 protein, which is a tumor suppressor involved in cell cycle regulation and apoptosis.
Automatically generated - may contain errors
2 protocols using anti p53 sc 98
1
Western Blot Analysis of Protein Expression
2
Western Blot Analysis of Protein Expression
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Cell extracts were prepared using RIPA buffer (20mM Tris, pH 7.4, 137 mM NaCl, 1% NP-40, 0.25% Deoxycholate, 0.1% SDS, 1mM EDTA and 1% protease inhibitor cocktail). Protein concentration was determined by BCA kit (Pierce; Rockford, IL) and 100 µg protein was analyzed by Western blotting as described previously69 (link). Anti-p53 (sc-98, Santa Cruz) was used at 1:1000 (2 µg/mL) dilution and anti-Bcl-2 (sc-492, Santa Cruz) and anti-β-actin (Sigma) were used at 1:1000 dilutions. SPDEF antibodies (gift from Dr. Jeffery Whitsett, Children’s Hospital, University of Cincinnati, OH) were used at 1:5000 dilution. Proteins were detected using ECL and visualized by chemiluminescence (Perkin Elmer, Waltham, MA) using the FujiFilm Image Reader LAS-4000 (Valhalla, NY). The uncropped scans of the most important Western blots are included as a supplementary figure in the Supplementary Information.
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